Аннотация

Tea fungus (Medusomyces gisevii L.) is a natural symbiotic consortium of yeast-like fungi and bacteria. Scientific literature provides a lot of information about the consortium, but it is largely fragmentary. We aimed to review and systematize the information on the research topic.
We studied scientific publications, conference proceedings, intellectual property, regulatory documents, and Internet resources on the M. gisevii consortium using Scopus, Web of Science, e.LIBRARY.RU, and Google Academy. The methods applied included registration, grouping, classification, comparative analysis, and generalization.
We described the origin and composition of tea fungus, specifying the microorganisms that make up its symbiotic community depending on the place of origin. Then, we reviewed the stages of fermentation and cultivation conditions in various nutrient media and presented the composition of the culture liquid. Finally, we analyzed the antimicrobial effect of M. gisevii on a number of microorganisms and delineated some practical uses of the fungus.
The data presented in this article can be used to analyze or develop new methods for the cultivation and application of M. gisevii. We specified some possibilities for using not only the culture liquid but also the fruit body of the fungus in various industries.

Ключевые слова

Kombucha, Medusomyces gisevii, composition, cultivation, application

ВВЕДЕНИЕ

Kombucha, a fermented tea drink, is reported to have been first mentioned as early as 220 BC [1]. Originating in Manchuria (China), it was brought to Japan in 414 AD, and then to Eastern Europe and America. Kombucha appeared in Russia and Ukraine during the Russo-Japanese War of 1904–1905 [2]. During the Second World War, the drink was brought to Germany, and in the 1950s, to France, as well as North Africa, where it became quite popular. In the postwar years, its popularity reached its peak in Italy and Switzerland, where the drink was found as beneficial as yogurt [3].

Literature offers a variety of names for the kombucha drink based on Medusomyces gisevii L., including “Manchurian mushroom”, “Japanese mushroom”, “Japanese sponge”, “sea mushroom” or simply “mushroom”, “kvass” or “tea kvass”, “fango”, “kombuha”, “Indian tea mushroom”, “miraculous mushroom”, “tea fungus”, “kam-boo-ha”, “Scoby”, “Hongo”, and many others [1, 4, 5]. The book Kombucha by Gunter W. Frank boasts as many as 86 synonyms for the drink’s name.

Historically, the kombucha culture liquid has long been used in traditional medicine not only as a refreshing drink, but also to heal various diseases.

Today, kombucha is sold in retail stores, and the M. gisevii culture can be purchased online [1, 6].

According to State Standard STB 1818-2007 of the Republic of Belarus, “Functional Food Products. Terms and Definitions”, a functional food product is a product that is intended for systematic use as part of a diet in all age groups of a healthy population to reduce the risk of developing diseases associated with nutrition, as well as to maintain and improve health due to the presence of physiologically functional ingredients.

Functional food products can be divided into several groups (Fig. 1).

According to Fig. 1, a drink based on the M. gisevii microbiological community can be classified as a functional product.

M. gisevii (medusomycete) is a symbiotic community of yeast-like fungi and bacteria that forms a thick, leathery, amorphous layered film of tea fungus on the surface of fermentable nutrient solutions (tea extract, juice, etc.) [7].

It is quite common to cultivate tea fugus at home and use its culture liquid, or fermentation product, as a drink (kombucha). This drink is widely known as a valuable preventative and medicinal remedy for various diseases. Its popularity is fueled by recommendations given in popular science articles and publications on traditional medicine. However, scientific literature also reports its negative impact on the body [1, 8]. Therefore, we found it important to study the chemical composition of the M. gisevii fermented culture liquid and to systematize the materials available on it.

ОБЪЕКТЫ И МЕТОДЫ ИССЛЕДОВАНИЯ

Our study was carried out at the Department of Biotechnology, the Faculty of Technology of Organic Substances, Belarusian State Technological University. We studied scientific articles, conference proceedings, intellectual property, regulatory documents, and Internet resources over a period from 1989 to 2022. They were selected from the bibliographic databases of Scopus, Web of Science, eLIBRARY.RU, and Google Academy by using Medusomyces gisevii L. and kombucha as keywords. We analyzed the data by employing such methods as registration, grouping, classification, as well as comparative analysis and generalization.

РЕЗУЛЬТАТЫ И ИХ ОБСУЖДЕНИЕ

Composition. T he fi rst s cientific i nformation a bout the Medusomyces gisevii L. microflora appeared in the articles of Gustav Lindau (1913). Its species composition is diverse and dependent on the conditions, place, and time of cultivation. The M. gisevii symbiont is composed of culture liquid, zooglea, mesoglea, and sediment [7, 9, 10].

The zooglea of M. gisevii is a complex structural formation of bacterial cellulose in which various microorganisms are immobilized. It is based on the colonies of acetic acid bacteria Gluconacetobacterim, Acetobacter, Lactococcus, Lactobacillus, and Clostridium, as well as yeasts Saccharomyces, Bretanomyces, Torulopsis, Zygosaccharomyces, Schizosaccharomyces, Candida, and others [4, 7, 9–11].

Amarasinghe et al. identified the following components in the zooglea: Acetobacter xylinum, Acanthodica xylinoides, Acetobacter aceti, Acetobacter pausterianus, Bacterium gluconicum, Kloeckera spp., Schizosaccharomyces pombe, Saccharomyces ludwigii, Saccharomyces cerevisiae, Torulaspora spp., Zygosaccharomyces bailii, and Pichia spp.

In the study by Savary et al., the zooglea was composed of Dekkera bruxellensis, Hanseniaspora uvarum, Acetobacter okinawensis, and Liquorilactobacillus nagelii [13]. The patent by Chekasina et al. described the following consortium: Saccharomyces mandshuricus, Hausemaspora sp., Torulopsis globosa, Torulopsis sp., S. ludwigii, Saccharomyces lactis, A. xylinum, A. aceti, Gluconobacter subaxydans, and other microorganisms [14].

Bayramaliyeva et al. isolated thirteen strains of yeast from the M. gisevii fruit body and culture liquid, as well as characterized their morphological and cytological features [15].

Thus, the M. gisevii symbiosis can have a very diverse composition.

Quorum sensing is a mechanism that establishes a balance between the microorganisms of a symbiont [9]. It enables a symbiotic community to form, depending on the combination of certain factors. In addition, the symbiotic community contains about 1–10% of so-called “persister” microorganisms. These are cells which are at rest and represent a protective and adaptive life form.

The color of the zooglea is determined by the tea extract or other coloring components of the nutrient medium. The zooglea is kept on the surface of the culture liquid due to carbon dioxide and partly due to the edge adhesion to the vessel, with a film growing at rest without layers. When the film gets damaged, it forms a new layer on the surface of the old one, resulting in layers. The synthesis of bacterial cellulose begins after the symbiont is placed in the nutrient medium and proceeds through several stages: 1) consolidation of bacteria with associates forming on the surface of the culture liquid; 2) synthesis of cellulose microfibrils and the formation of multilayer structures of bacterial cellulose on the surface of the medium; 3) their colonization by microorganisms; and 4) the activity of symbiont microorganisms. Bacterial cellulose forms throughout the entire cultivation period, although it depends on many factors (substrate, extract, inoculum, temperature, pH, etc.).

Therefore, the metabolic activity of M. gisevii’s enzymatic systems depends on the activity of microorganisms in the culture liquid that are immobilized in bacterial cellulose. Bacterial cellulose is synthesized by the acetic acid bacteria Gluconacetobacter xylinus with the help of monosaccharides (glucose or its phosphorylated forms) [9, 16]. It is formed by Gram-negative bacteria of the genera Komagataeibacter (Gluconacetobacter), Agrobacterium, Achromobacter, Enterobacter, Rhizobium, Pseudomonas, Salmonella, Azotobacter, and Alcaligenes, as well as by Gram-positive bacteria Sarcina ventriculi and Rhodococcus. The best known producer of bacterial cellulose is the acetic bacterium Komagataeibacter xylinus (G. xylinus, A. xylinum, Acelobacter aceti ssp. xylinum, Acetobacter xylinus).

The inner part of bacterial cellulose is a multilayer structure of microfibrils, in which nutrient substrates, enzymes, microorganisms and their metabolic products move due to diffusion.

Mesoglea is made of filamentous formations of bacterial cellulose containing symbiont microorganisms. It is most actively formed in extracts of black and green teas at low positive temperatures (< 10°C) [9].

The sediment of the culture medium is usually localized at the bottom of the vessel as a dense brown mass [9, 10]. It consists of resins, tannins, tea dust, yeasts, and bacteria that stop growing in the presence of 4–5% of sugars. This is due to the accumulation of microbial products (e.g., organic acids) in the culture liquid.

The fungal biomass contains crude protein (45–53%), carbohydrates (37–43%), including polysaccharides (23–26%), lipids (8–11%), nucleic acids (7–9%), minerals (8–10%), vitamins (C, B₁, PP – 0.11–0.54%), amino acids (28–33%), and microelements (2.1–3.7%). The microelements (mg/kg) include potassium (14025), sodium (5790), magnesium (1379), iron (1017), copper (31.7), zinc (70.5), manganese (342), and chromium (10.5) [17].

The amino acids in the biomass protein (%) include lysine (2.01), histidine (1.75), arginine (2.05), aspartic acid (3.54), trionine (1.41), serine (1.39), glutamic acid (4.07), proline (1.97), alanine (1.97), cystine (0.21), valine (1.73), methionine (0.45), isoleucine (1.57), leucine (2.23), tyrosine (1.23), and phenylalanine (1.93) [17].

Fermentation process. Kombucha is produced by three types of fermentation: lactic acid, alcoholic, and acetic [4, 7]. During lactic acid fermentation, glucose is decomposed to lactic acid under the action of lactic acid bacteria. Alcoholic fermentation leads to the decomposition of glucose to ethyl alcohol by yeast, with carbon dioxide emitting during the process. Acetic fermentation converts ethyl alcohol into acetic acid and water by oxygen and acetic bacteria [18].

Fermentation also produces several intermediate substances, including phosphoric acid. It plays an important role in the formation of phosphoric acid esters, which are converted into free pyruvic acid.

In addition, fermentation leads to an increase in polyphenols and flavonoids in the culture liquid. Thearubigin turns into theaflavin and the culture liquid changes its color from dark to light during the cultivation of the fungus [19, 20].

Catechins present in tea can be converted by symbiont microorganisms to simpler components, which increases the antioxidant power of the culture liquid [19–21].

Cultivation conditions. Since the M. gisevii microorganisms can be found on the surface of plants, they can be grown on artificial nutrient media, using extracts of various types of tea and other plants [9]. Their normal growth requires a nutrient medium that satisfies the needs of the symbiotic community. Such a medium may include leaves of black and green tea, rooibos (Aspalathus linearis), fireweed (Epilobium), lemon balm (Melissa officinalis), common oak (Quercus robur), common blueberry (Vaccinium myrtillus), fragrant callisia (Callisia fragrans), herniaria (Herniaria), acacia (Acacia), gray myrobalan (Phyllanthus emblica), Bengal quince (Aegle marmelos), woolly erva (Aerva lanata), cassia (Cassia auriculata), common barley (Hordeum vulgare), mint (Mentha), common thyme (Thymus vulgaris), nettle (Urtica), savory (Satureja), turmeric (Curcuma xanthorrhiza), and other plants [1, 19, 22–26].

The main substrates of M. gisevii are carbohydrates represented mainly by mono- and oligosaccharides (glucose, fructose, galactose, mannitol, xylose, sucrose, maltose, etc.), alcohols, organic acids, and other substances [27]. Galactose is less preferable as a nutrient substrate, while sucrose and lactose are better assimilated. The use of lactose results in minimal formation of bacterial cellulose. Glucose promotes the synthesis of bacterial cellulose and gluconic acid, especially at a concentration of 10–20 g/L. Replacing glucose with maltose leads to a 10-fold decrease in bacterial cellulose [9].

Voon et al. analyzed tea fungus cultivation with white refined sugar, coconut sugar, and sugar molasses [28]. They reported a significantly larger fungal biomass when using refined sugar. The culture liquid based on sugar molasses contained the largest amount of organic acids, while the culture liquid based on coconut sugar showed better antioxidant activity and a higher phenol content.

During cultivation, yeast oxidizes carbohydrates to ethyl alcohol and carbon dioxide, and bacteria complete the oxidation of ethanol to acetic acid. The acid accumulates during bacterial cultivation and affects the pH value. During symbiont cultivation, the nutrient medium is saturated with ethanol and acetic acid, thus protecting the symbiotic community from contamination by foreign microflora [9].

When microorganisms actively consume the nutrient substrate, the culture medium becomes acidified. This process depends on the temperature and the presence of various additives in the medium, such as alcohols (ethanol and glycerol). Glycerol in low concentrations serves as a source of carbon for the synthesis of bacterial cellulose, but in high concentrations (> 20%) it inhibits this process [9]. Ethanol (1.0–1.5%) can accelerate the synthesis of bacterial cellulose and be used as an alternative carbon source [29].

In addition to the above alcohols, ethylene glycol, propanol, butanol, methanol, and mannitol are used to stimulate the biosynthesis of bacterial cellulose. These additives can increase the yield of bacterial cellulose by 13.4–56.0%. However, carbohydrates at concentrations of 5–10% are preferable for production purposes. Also, food industry wastes such as molasses and distillery waste are recommended as cheap substrates [9]. Organic acids (lactic, pyruvic, malic, acetic, succinic, and citric) added to the main substrate increase the yield of bacterial cellulose by 1.4–1.9 times [30]. Specialized media have proven effective for tea fungus cultivation, which, in addition to carbohydrates, contain salts ((NH₄)₂SO₄, KH₂PO₄, MgSO₄, FeSO₄, CaCl₂, NaMoO₄, ZnSO₄, MnSO₄, CuSO₄, etc.), vitamins (PP, B₁, B₂, B₃, B₆, H), and aminobenzoic acid [9].

Gladysheva et al. produced bacterial cellulose by bioconversion of M. gisevii on a synthetic nutrient medium containing sucrose, black tea extract, starch hydrolysate, and enzymatic miscanthus hydrolysate [31, 32]. Although this medium is not optimal for the biosynthesis of bacterial cellulose, IR spectroscopy established that the bacterial cellulose obtained was a chemically pure compound containing only cellulose. This indicated a high adaptive potential of the symbiotic culture of M. gisevii.

According to Skiba et al., the highest yield of bacterial cellulose (7.5–8.0%) was provided at an inoculum amount of 10–20% vol., although all the amounts under study produced bacterial cellulose samples with the same three-dimensional microfibrillar structure [33]. The authors found that the inoculum amounts and the duration of biosynthesis affected the degree of polymerization. Thus, the process of biosynthesis can be controlled to synthesize bacterial cellulose with a given degree of polymerization. 

Pribilsky et al. pointed to the importance of water pretreatment for fungus cultivation. In their study, filtered water was disinfected and treated with natural minerals (flint, carnelian, opal chalcedony, quartz) [34]. The minerals were placed in water for the entire fermentation process to promote fungal growth and inhibit foreign microorganisms.

The patent by Skripitsyna described a method for obtaining drinks from fermented vegetable extracts with spices and salt [6]. For this, vegetable juice was fermented with the culture liquid of M. gisevii.

Another method of cultivating fungus to obtain a soft drink was presented by Ogarkov et al. [35]. The authors mixed sugar, tea fungus concentrate, water-soluble melanin, and a water-alcohol solution of lemon balm with water in the presence of carbon dioxide and kept the mixture at 7–10°C.

Fungus zooglea can also be cultivated anaerobically, resulting in a drink with maximum biological activity [36, 37]. However, this method has two disadvantages, namely a decrease in dry matter and an increase in the cultivation time up to 150 days [36].

In addition, Skripitsyna and Zajtsev described methods for preparing beverages by successively fermenting sugar-containing products (water with sugar, jam, or honey) with yeast cultures (baking, wine, etc.) and cultivating tea fungus [6, 38].

Rogozhin et al. studied the effect of low temperatures on the cultivation of M. gisevii [10]. It is a fact that low temperatures inhibit the body’s metabolism. A short-term effect of low temperatures usually leads to a higher metabolic and functional activity than under normal conditions. However, their prolonged action, especially with temperatures below 4°С, can cause death in some living organisms. In another study, Rogozhin et al. cultivated M. gisevii in a black tea extract [39]. Low temperatures completely suppressed the activity of symbiont microorganisms, which manifested in stable pH values and electrical conductivity for 30–240 days. However, raising the temperatures increased the symbiont’s productivity, as shown by changes in the above indicators.

In the same study [39], M. gisevii was cultivated in coffee extracts at 8°C. During the first 30 days, symbiont microorganisms were at rest and then, during the following 60–240 days, their metabolic activity was increased by lower pH values and higher electrical conductivity of the culture liquid. This indicated that the coffee extract contained components that could activate microorganisms even at low temperatures. However, prolonged exposure to –20°C had a negative effect on the viability of M. gisevii, showing individual cryoprotective properties of black tea and coffee extracts. Furthermore, negative temperatures had different effects on the ability of M. gisevii to synthesize bacterial cellulose in black tea and coffee extracts. In the black tea extract, the symbiont’s enzymatic systems were less active, while in the coffee extract, they exhibited high activity, manifesting in a 1.84–3.92-fold increase in the zooglea mass [39].

Tea fungus should not be stored at low temperatures since it can lead to a so-called “malolactic transformation”. This means that malic acid, which is beneficial for the body, is converted into lactic acid, whose excess can cause muscle pain and fatigue. However, the study by Jayabalan et al. showed that heat treatment was not appropriate either for preserving tea fungus [40].

Marchenko and Sotnikov calculated the productivity of the fungus film during its cultivation [41]. The acid formation rate of the culture liquid was 0.03–0.08 ΔK/h, where K was the acidity of the fermented drink taken as the volume (cm³) of 0.1 mol/L of sodium hydroxide solution used to neutralize 100 cm³ of a culture liquid sample. Therefore, various methods are proposed to stimulate fungal growth, e.g., 8–10 g of dead bees (a source of chitin) per 1 liter of nutrient medium [15, 42]. This additive accelerates the production of bacterial cellulose, increases fungal biomass, and improves the taste of the resulting product.

A culture with hydrophilic properties immobilized on a carrier with a rough surface (chopped twigs, wood chips) can be used to reduce the cultivation time, increase productivity, and improve the quality of kombucha [41]. There is also a method for preparing a non-alcoholic beverage with a pear flavor based on tea fungus and fruit waste used as a substrate [43].

Composition of the Medusomyces gisevii culture liquid. During the symbiont cultivation, the culture liquid accumulates a large number of various components, including nutrient substrate residues and products of microbial activity moving due to diffusion. The culture liquid contains organic (acetic, gluconic, citric, oxalic, lactic, kojic, tartaric, pyruvic, L-lactic, D-sugar, usnic, malonic, malic, and succinic) acids, inorganic (phosphoric) acids, proteins, lipids (sterols, phosphatides, fatty acids), carbohydrates, vitamins (C, B, PP), pigments (chlorophyll, xanthophyll), enzymes (catalase, lipase, protease and carboxylase, amylase, tryptic enzymes), nucleic acids, nitrogenous bases, chitin, caffeine, amino acids, purine bases, polyphenols, ethanol, as well as various elements (zinc, copper, iron, manganese, nickel, cobalt) and even a natural antibiotic, jellyfish [4, 7, 17, 21, 23, 25, 26, 42, 44, 45].

During the cultivation of M. gisevii, the nutrient medium is saturated with ethanol and acetic acid, creating favorable conditions for natural protection of the symbiotic community from contamination by foreign microflora. However, at the initial stage of cultivation, the emerging bacterial cellulose can be damaged by various types of mold due to a high content of carbohydrates and tea extracts in the medium [9]. Therefore, it is recommended to add a small amount of the fermented culture liquid during the initial period of symbiont growth.

Ivanov et al. found glucuronic acid (0.037–1.390%) in the first 5 days of fungal growth, which was not detected later [17]. During 20 days of growth, the culture liquid (100 cm³) contained citric and malic acids (4 mg) and volatile acids (12 mg). The content of ethyl alcohol ranged from 0.15 to 0.7%. However, alcohol was not detected on the 30th day of growth. In addition, the culture liquid contained vitamins C and B, tannins (0.08%) and purine bases, a large number of resinous and fat-like substances insoluble in water and alcohol, proteins and nucleoproteins (5.24%), and a number of enzymes (amylase and catalase).

Antimicrobial properties of the culture liquid. M. gisevii has antimicrobial activity due to the presence of antibacterial substances in its culture liquid, which have both bacteriostatic and bactericidal properties. The culture liquid increases the size and volume of bacterial cells, which leads to changes in their shape, vacuolization, and the appearance of granular inclusions. Further, it decreases the intensity of redox processes in microbial cells, reduces their virulence, and increases immunogenicity [46, 47]. In the study [48] associated the antimicrobial activity of M. gisevii with the action of acetic acid (the main product of fermentation) on microorganisms. Many researchers [5, 25, 45, 46, 48–53] established the antibacterial efficacy of the M. gisevii culture liquid against various microorganisms (Table 1).

Noteworthily, not only acetic acid and large proteins, but also other molecular structures can be active components of the M. gisevii culture liquid [45].

Thus, although numerous studies have shown the antimicrobial effect of the M. gisevii culture liquid against a number of pathogenic microorganisms, they have not fully explained the exact mechanism of this effect.

Practical use. Depending on the nutrient substrate, the M. gisevii culture liquid can be used as a soft drink to prevent a wide range of diseases, such as hypertension, atherosclerosis, sleep disorders, liver problems, gastrointestinal disorders, and others [1, 2, 4, 5, 9, 12, 55–60]. Kombucha stimulates the endocrine and immunocompetent systems, limits atherosclerotic plaques, corrects body weight, increases the body’s resistance to carcinogenic factors, has a sedative effect, prevents and alleviates headaches, reduces alcohol dependence, as well as has antitumorous and probiotic effects in combination with ginger [1, 61, 62]. There is a formulation of a fermented beverage based not only on tea fungus but also on birch chaga fungus [37].

Bondareva et al. developed a biologically active substance with a prebiotic effect based on M. gysevii [57, 63]. This culture liquid is also used as a plant base for medicines with a pronounced healing and antiinflammatory effect [2, 64, 65]. The systematic intake of the fungal cultural liquid can improve the well-being of elderly people with severe symptoms of atherosclerosis and have a beneficial effect on intestinal atony and gastrointestinal diseases. The culture liquid can also reduce blood cholesterol levels due to a high content of gluconic acid. However, quite a few publications [8, 26] point to its negative effects on human health.

M. gisevii can be used as a biosorbent to remove heavy metals through the ion exchange mechanism [66, 67]. The symbiont is used to produce acetic acid on an industrial scale for the food industry, as well as to produce starter cultures for fermented milk products [10, 68]. Kombucha enriched with sea grapes (Caulerpa racemosa) has been proposed as a functional drink to combat obesity [69].

Bacterial cellulose is widely used in the production of pulp, paper, and paints, as well as in the fine chemical industry and electronics [10, 11, 32, 70]. Fine powders obtained from bacterial cellulose are used in the food industry as thickeners and gelling agents [9, 10]. Unlike plant cellulose, bacterial cellulose is pure and free of lignin, hemicellulose, and other impurities [53]. Therefore, a biofilm can serve as a matrix for immobilizing various inorganic compounds (ions of silver, selenium, magnesium, cobalt, manganese, etc.) and biogenic molecules (peptides, amino acids, proteins, enzymes, vitamins, hormones, antibiotics, etc.). Depending on the immobilized components, the film can then be used in medicine and/or pharmaceutics, e.g., to treat burns and ulcers, as well as postoperative, purulent, and traumatic wounds [16, 56, 59, 71]. Biofilms with immobilized bioactive substances can also be applied in cosmetology to restore skin elasticity. The Siberian Federal University in cooperation with the Institute of Biophysics (Siberian Branch of the Russian Academy of Sciences) use bacterial cellulose as a substrate for growing various tissue engineering structures. Since bacterial cellulose is non-toxic and non-allergic, as well as has a high absorption capacity, it can be used as an adsorbent to stimulate digestion.

In bakery, the M. gisevii culture liquid is added to the dough to activate its maturation and increase the calorie content of the finished product [10, 72, 73].

Tea fungus can be used as a feed additive for animals. For example, in Tatarstan, a preparation containing M. gisevii biomass and/or culture liquid is used for feeding birds [17]. Dried biomass can also be applied for the same purpose [13].

The fruit body of tea fungus is used to create ecofriendly clothing and to produce valuable bacterial cellulose by recovering hydrocarbon waste [74].

The Department of Biotechnology at the Belarusian State Technological University (Minsk, Republic of Belarus) has been developing a preparation based on the fruit body of tea fungus to stimulate plant growth.

ВЫВОДЫ

Based on the above, we can conclude that the Medusomyces gisevii L. culture liquid is an excellent prophylactic against cardiovascular and gastrointestinal diseases. It can help treat atherosclerosis, acute tonsillitis, arterial hypertension, and other diseases. However, there is still a lack of research into the composition and properties of the culture liquid, as well as methods for its cultivation. Therefore, new works are published each year that open up new possibilities for the use of M. gisevii. We analyzed the prospects for using not only the culture liquid but also the fruit body of M. gisevii in the food, pharmaceutical, and other industries. In addition, the materials collected from numerous studies can be used to create an optimal technology for its cultivation.

Вклад авторов

O.S. Ermakova collected information and E.A. Flyurik processed it.

КОНФЛИКТ ИНТЕРЕСОВ

The authors declare that they have no conflict of interest.

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