ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Pig adipose tissue of two different breeds and locations: morphology and Raman studies

Аннотация
According to the recent data, there are 4–5-local pig breeds left in Russia by now. Livni is among them. This breed is characterized by high fat content. Back fat has been analyzed earlier. We aimed to assess fat morphometrics from other localizations in pigs.
Sacral, axillary, and perirenal fat samples from 6-month-old Duroc and Livni pig breeds were analyzed using morphological and Raman-based techniques.
Livni adipocytes were characterized by dense packing with a polyhedron-like structure. In Duroc fat, they were more rounded (spherical). A “two-phase” cell disperse was identified in all samples. Fat cells in Livni pigs were bigger than those in the Duroc breed: 70–102%; 15–18 and 26% for sacral, axillary, and perirenal locations. Differences in the intensity of the Raman signal between the samples were found: in the samples of subcutaneous adipose tissue, more intense peaks were observed, which are responsible for unsaturation; the samples of Livni axillary fat were characterized by greater unsaturation than sacral fat.
Livni and Duroc adipocytes differ from each other in form and size and the difference depends on location. Pork fat from local breeds is expected to have potentially more health protecting (for animals) and health promoting (for consumers) properties.
Ключевые слова
Fat, pigs, Duroc, Livni, histology, adipocytes, morphometry, Raman spectroscopy
Вклад авторов
The authors were equally involved in writing the manuscript and are equally responsible for plagiarism.
ФИНАНСИРОВАНИЕ
This work was financially supported by the Russian Science Foundation (RSF) (project No. 21-76-20032).
СПИСОК ЛИТЕРАТУРЫ
  1. Giuffra E, Kijas JMH, Amarger V, Carlborg O, Jeon J-T, Andersson L. The origin of the domestic pig: Independent domestication and subsequent introgression. Genetics. 2000;154(4):1785–1791. https://doi.org/10.1093/genetics/154.4.1785
  2. Mikhailova OA. Tendencies of the world swine breeding development. Bulletin of Agrarian Science. 2018;70(1):36–45. (In Russ.). https://doi.org/10.15217/issn2587-666X.2018.1.36
  3. Traspov A, Deng W, Kostyunina O, Ji J, Shatokhin K, Lugovoy S, et al. Population structure and genome characterization of local pig breeds in Russia, Belorussia, Kazakhstan and Ukraine. Genetics Selection Evolution. 2016;48(1). https://doi.org/10.1186/s12711-016-0196-y
  4. Mikhailov NV, Baranikov AI, Svinarev IYu. Pig breeding. Pork production technology. Rostov-on-Don: Izdatelʹstvo Yug; 2009. 417 p. (In Russ.).
  5. Genetics made in Russia. The import of purebred breeding pigs has decreased by 3.7 times over five years [Internet]. [cited 2022 Aug 8]. Available from: https://www.agroinvestor.ru/technologies/article/30688-genetika-made-in-russia
  6. Kharzinova VR, Zinovieva NA. The pattern of genetic diversity of different breeds of pigs based on microsatellite analysis. Vavilov Journal of Genetics And Breeding. 2020;24(7):747–754. (In Russ.). https://doi.org/10.18699/VJ20.669
  7. Quan J, Gao C, Cai Y, Ge Q, Jiao T, Zhao S. Population genetics assessment model reveals priority protection of genetic resources in native pig breeds in China. Global Ecology and Conservation. 2020;21. https://doi.org/10.1016/j.gecco.2019.e00829
  8. Gan M, Shen L, Fan Y, Guo Z, Liu B, Chen L, et al. Altitude adaptability and meat quality in Tibetan pigs: A reference for local pork processing and genetic improvement. Animals. 2019;9(12). https://doi.org/10.3390/ani9121080
  9. Pugliese C, Sirtori F. Quality of meat and meat products produced from southern European pig breeds. Meat Science. 2012;90(3):511–518. https://doi.org/10.1016/j.meatsci.2011.09.019
  10. Brossard L, Nieto R, Charneca R, Araujo JP, Pugliese C, Radović Č, et al. Modelling nutritional requirements of growing pigs from local breeds using InraPorc. Animals. 2019;9(4). https://doi.org/10.3390/ani9040169
  11. Pavlova SV, Kozlova NA, Shchavlikova TN. Breeding base of pig breeding in Russia at the beginning of 2021. Efficient Animal Husbandry. 2021;171(5):28–31. (In Russ.).
  12. Directions of productivity [Internet]. [cited 2022 August 08]. Available from: https://www.websadovod.ru/pig/03.htm
  13. Bekenev VA, Arishin AA, Mager SN, Bolshakova IV, Tretyakova NL, Kashtanova EV, et al. Lipid profile of pig tissues contrasting in meat production. Natural Products Journal. 2021;11(1):108–118. https://doi.org/10.2174/2210315509666191203124902
  14. Poklukar K, Čandek-Potokar M, Lukač NB, Tomažin U, Škrlep M. Lipid deposition and metabolism in local and modern pig breeds: A review. Animals. 2020;10(3). https://doi.org/10.3390/ani10030424
  15. Monziols M, Bonneau M, Davenel A, Kouba M. Comparison of the lipid content and fatty acid composition of intermuscular and subcutaneous adipose tissues in pig carcasses. Meat Science. 2007;76(1):54–60. https://doi.org/10.1016/j.meatsci.2006.10.013
  16. Sturm G, Susenbeth A, Ehrensvärd U, Gmelin M, Loeffler K. The adaptation of the morphometric parameters of fat cell size for the purposes of animal science research. 2. Cellularity of subcutaneous adipose tissue of different swine breeds influenced by graduated feed levels and in relation to metabolic data and parameters of body fat degeneration. Berliner und Munchener tierarztliche Wochenschrift. 1990;103(4):112–117.
  17. Chernukha IM, Fedulova LV, Kotenkova EA. White, beige and brown adipose tissue: structure, function, specific features and possibility formation and divergence in pigs. Foods and Raw Materials. 2022;10(1):10–18. https://doi.org/10.21603/2308-4057-2022-1-10-18
  18. Hauser N, Mourot J, De Clercq L, Genart C, Remacle C. The cellularity of developing adipose tissues in Pietrain and Meishan pigs. Reproduction. Nutrition. Development. 1997;37(6):617–625. https://doi.org/10.1051/rnd:19970601
  19. Nakajima I, Oe M, Ojima K, Muroya S, Shibata M, Chikuni K. Cellularity of developing subcutaneous adipose tissue in Landrace and Meishan pigs: Adipocyte size differences between two breeds. Animal Science Journal. 2011;82(1):144–149. https://doi.org/10.1111/j.1740-0929.2010.00810.x
  20. Vincent A, Louveau I, Gondret F, Lebret B, Damon M. Mitochondrial function, fatty acid metabolism, and immune system are relevant features of pig adipose tissue development. Physiological Genomics. 2012;44(22):1116–1124. https://doi.org/10.1152/physiolgenomics.00098.2012
  21. Ayuso D, González A, Peña F, Hernández-García FI, Izquierdo M. Effect of fattening period length on intramuscular and subcutaneous fatty acid profiles in Iberian pigs finished in the Montanera sustainable system. Sustainability. 2020;12(19). https://doi.org/10.3390/su12197937
  22. Beattie JR, Bell SEJ, Borgaard C, Fearon A, Moss BW. Prediction of adipose tissue composition using Raman spectroscopy: Average properties and individual fatty acids. Lipids. 2006;41(3):287–294. https://doi.org/10.1007/s11745-006-5099-1
  23. Romeis B. Mikroskopische technik. München: Urban u. Schwarzenberg; 1989. 697 p.
  24. Velotto S, Vitale C, Crasto A. Muscle fibre types, fat deposition and fatty acid profile of Casertana versus Large White pig. Animal Science Papers and Reports. 2012;30(1):35–44.
  25. Berhe DT, Eskildsen CE, Lametsch R, Hviid MS, van den Berg F, Engelsen SB. Prediction of total fatty acid parameters and individual fatty acids in pork backfat using Raman spectroscopy and chemometrics: Understanding the cage of covariance between highly correlated fat parameters. Meat Science. 2016;111:18–26. https://doi.org/10.1016/j.meatsci.2015.08.009
  26. Louveau I, Perruchot M-H, Bonnet M, Gondret F. Invited review: Pre- and postnatal adipose tissue development in farm animals: From stem cells to adipocyte physiology. Animal. 2016;10(11):1839–1847. https://doi.org/10.1017/S1751731116000872
  27. Zhao J, Tao C, Chen C, Wang Y, Liu T. Formation of thermogenic adipocytes: What we have learned from pigs. Fundamental Research. 2021;1(4):495–502. https://doi.org/10.1016/j.fmre.2021.05.004
  28. Abbas O, Fernández Pierna JA, Codony R, von Holst C, Baeten V. Assessment of the discrimination of animal fat by FT-Raman spectroscopy. Journal of Molecular Structure. 2009;924–936:294–300. https://doi.org/10.1016/j.molstruc.2009.01.027
  29. Saleem M, Amin A, Irfan M. Raman spectroscopy based characterization of cow, goat and buffalo fats. Journal of Food Science and Technology. 2021;58(1):234–243. https://doi.org/10.1007/s13197-020-04535-x
  30. Czamara K, Majzner K, Pacia MZ, Kochan K, Kaczor A, Baranska M. Raman spectroscopy of lipids: A review. Journal of Raman Spectroscopy. 2014;46(1):4–20. https://doi.org/10.1002/jrs.4607
  31. De Gelder J, De Gussem K, Vandenabeele P, Moens L. Reference database of Raman spectra of biological molecules. Journal of Raman Spectroscopy. 2007;38(9):1133–1147. https://doi.org/10.1002/jrs.1734
  32. Afseth NK, Dankel K, Andersen PV, Difford GF, Horn SS, Sonesson A, et al. Raman and Near Infrared Spectroscopy for quantification of fatty acids in muscle tissue – A salmon case study. Foods. 2022;11(7). https://doi.org/10.3390/foods11070962
  33. Determining pork fat quality as measured by three methods with an industry standard marketing plan for pigs fed 20% DDGS [Internet]. [cited 2022 August 08]. Available from: https://porkcheckoff.org/wp-content/uploads/2021/02/12-045-WIEGAND-UofMO.pdf
  34. Lee J-Y, Park J-H, Mun H, Shim W-B, Lim S-H, Kim M-G. Quantitative analysis of lard in animal fat mixture using visible Raman spectroscopy. Food Chemistry. 2018;254:109–114. https://doi.org/10.1016/j.foodchem.2018.01.185
  35. Bee G, Gebert S, Messikommer R. Effect of dietary energy supply and fat source on the fatty acid pattern of adipose and lean tissues and lipogenesis in the pig. Journal of Animal Science. 2002;80(6):1564–1574. https://doi.org/10.2527/2002.8061564x
  36. Pchelkina VA, Chernukha IM, Fedulova LV, Ilyin NA. Raman spectroscopic techniques for meat analysis: A review. Theory and Practice of Meat Processing. 2022;7(2):97–111. https://doi.org/10.21323/2414-438X-2022-7-2-97-111
Как цитировать?
Pchelkina VA, Chernukha IM, Nikitina MA, Ilin NA. Pig adipose tissue of two different breeds and locations: morphology and Raman studies. Foods and Raw Materials. 2023;11(1):1–9. https://doi.org/10.21603/2308-4057-2023-1-547
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