ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Indigenous yeast with cellulose-degrading activity in napa cabbage (Brassica pekinensis L.) waste: Characterisation and species identification

Аннотация
Napa cabbage waste contains an organic component, cellulose, which can be utilised as an ingredient for cellulose-degrading enzyme production with the help of indigenous yeast. The aim of the research was to identify and characterise potential indigenous yeast isolated from napa cabbage waste, which has cellulose-degrading activity. Indigenous yeast were isolated and characterised using the RapID Yeast Plus System, then turbidity was used to determine the yeast total population. Indigenous yeast was grown at napa cabbage waste at 27, 37, and 40°C for three days, and cellulose-degrading activity was determined by the Dinitrosalicylic Acid (DNS) method. The potential yeast isolate with the highest cellulose-degrading activity was identified by a sequence analysis of the rRNA gene internal transcribed spacer (ITS) region with using primers ITS1 (5′-TCCGTAGGTGAACCTGCGG-3′) and ITS4 (5′- TCCTCCGCTTATTGATATGC-3′). The results were compared to the GenBank database using the Basic Local Alignment Search Tools/BLAST algorithm. Three species of indigenous yeast were isolated from napa cabbage waste (S2, S6, and S8). S8, incubated at 37ºC for three days, demonstrated the highest cellulose-degrading enzyme activity (1.188 U/mL), with the average activity of 0.684U/mL. Species identification results indicated that the S8 isolate had a 100% similarity to Pichia fermentans UniFGPF2 (KT029805.1).
Ключевые слова
Pichia fermentans , temperature , cellulase enzyme , internal transcribed spacer
СПИСОК ЛИТЕРАТУРЫ
  1. Directorate General of Horticulture Statistik Produksi Hortikultura Tahun 2014. Kementerian pertanian: 2015. 315 p. (In Indonesian).
  2. Rahmah A, Izzati M, Parman S. The Effect of Liquid Organic Fertilizer Based on Basic Sawi Putih (Brassica chinensis L.) Waste on Growth of Sweet Corn Plant. Buletin Anatomi dan Fisiologi, 2014;22(1):65–71. DOI: https://doi.org/10.14710/baf.v22i1.7810.
  3. Saha BC. Lignocellulose Biodegradation and Applications in Biotechnology. In: Saha BC, Hayashi K, editors. Lignocellulose Biodegradation. American Chemical Society; 2004. pp. 2–34. DOI: https://doi.org/10.1021/bk-2004-0889.ch001.
  4. Beltran G, Rozes N, Mas A, Guillamon JM. Effect of low-temperature fermentation on yeast nitrogen metabolism. World Journal of Microbiology and Biotechnology. 2007;23(6):809–815. DOI: https://doi.org/10.1007/s11274-006-9302-6.
  5. Kanti A, Sudiana IM. Aktivitas CMC-ase khamir Candida sp. Yang diisolasi dari tanah kebun biologi wamena, Papua [CMC-ase Activity of Yeast Candida sp., Isolated from Soil of Wamena Biological Gardens, Papua]. Berita Biologi [Biology News]. 2003;6(5):655–660. (In Indonesian).
  6. Utama GL, Kurnani TBA, Sunardi, Balia RL. The Isolation and Identification of Stress Tolerance Ethanol-fermenting Yeasts from Mozzarella Cheese Whey. International Journal on Advanced Science, Engineering and Information Technology. 2016;6(2):252–257. DOI: http://doi.org/10.18517/ijaseit.6.2.752.
  7. Balia RL, Kurnani TBA, Utama GL. Selection of Mozzarella Cheese Whey Native Yeasts with Ethanol and Glucose Tolerance Ability. International Journal on Advanced Science, Engineering and Information Technology.2018;8(4):1091–1097. DOI:http://doi.org/10.18517/ijaseit.8.4.5869.
  8. Utama GL, Sidabutar FEE, Felina I, Wira DW, Balia RL. The utilization of fruit and vegetable wastes for bioethanol production with the inoculation of indigenous yeasts consortium. Bulgarian Journal of Agricultural Science. 2019;25(2):264–270.
  9. Diana L, Lasmini T. Isolasi dan Identifikasi Khamir Selulolitik Dari Tanah Rizosfer Anggrek Puser Bumi (Pecteilis susannae L.) di Hutan Wonosadi Gunung Kidul DIY [Isolation and Identification of Cellulolytic Yeast from Soil Rizosphere Puser Earth Orchid (Pecteilis susannae L.) in Wonosadi Forest Gunung Kidul DIY]. Biogenesis: Jurnal Ilmiah Biologi [Scientific Journal of Biology]. 2016;4(1):21–28. (In Indonesian). DOI: https://doi.org/10.24252/bio.v4i1.1116.
  10. Gupta P, Samant K, Sahu A. Isolation of Cellulose-Degrading Bacteria and Determination of Their Cellulolytic Potential. International Journal of Microbiology. 2012;2012. DOI: http://doi.org/10.1155/2012/578925.
  11. Sulman S, Rehman A. Isolation and Characterization of Cellulose Degrading Candida tropicalis W2 from Environmental Samples. Pakistan Journal of Zoology. 2013;45(3):809–816.
  12. Asliha I.N, Alami NH. Karakterisasi Khamir dari Pulau Poteran Madura [Characterization of yeast from Poteran Madura Island]. Jurnal Sains dan Seni ITS [Journal of Science and Art in ITS]. 2014;3(2):E49–E52. (In Indonesian). DOI: https://doi.org/10.12962/j23373520.v3i2.6869.
  13. Lopez S, Mateo JJ, Maicas S. Characterisation of Hanseniaspora Isolates with Potential Aroma-enhancing Properties in Muscat Wines. South African Journal of Enology and Viticulture. 2014;35(2):292–303.
  14. Mateo JJ, Peris L, Ibanez C, Maicas S. Characterization of glycolytic activities from non-Saccharomyces yeasts isolated from Bobal musts. Journal of Industrial Microbiology & Biotechnology. 2011;38(2):347–354. DOI: https://doi.org/10.1007/s10295-010-0780-z.
  15. Latouche GN, Daniel HM, Lee OC, Mitchell TG, Sorrell TC, Meyer W. Comparison of use of phenotypic and genotypic characteristics for identification of species of the anamorph genus Candida and related teleomorph yeast species. Journal of Clinical Microbiology. 1997;35(12):3171–3180.
  16. Shuler ML. Kargi F. Bioprocess Engineering: Basic Concepts, 2nd ed. Prentice Hall; 2001. 576 p.
  17. Nguong DLS, Jun LY, Yatim NI, Nathan S, Murad AMA, Mahadi NM, et al. Characterising Yeast Isolates from Malaysia towards the Development of Alternative Heterologous Protein Expression Systems. Sains Malaysiana. 2011;40(4):323–329.
  18. Ganzle MG, Ehmann M, Hammes WP. Modeling of growth of Lactobacillus sanfranciscensis and Candida milleri in response to process parameters of sourdough fermentation. Applied and Environmental Microbiology. 1998;64(7):2616–2623.
  19. Fennema OR. Food Chemistry, Third Edition. Taylor & Francis; 1996. 1067 p.
  20. Samaranayake YH, Samaranayake LP. Candida krusei: biology, epidemiology, pathogenicity and clinical manifestations of an emerging pathogen. Journal of Medical Microbiology. 1994;41(5):295–310. DOI: https://doi.org/10.1099/00222615-41-5-295.
  21. López S, Mateo JJ, Maicas S. Screening of Hanseniaspora Strains for the Production of Enzymes with Potential Interest for Winemaking. Fermentation. 2015;2(1). DOI: https://doi.org/10.3390/fermentation2010001.
  22. Lin Y, Zhang W, Li CJ, Sakakibara K, Tanaka S, Kong HN. Factors affecting ethanol fermentation using Saccharomyces cerevisiae BY4742. Biomass & Bioenergy. 2012;47:395–401. DOI: https://doi.org/10.1016/j.biombioe.2012.09.019.
  23. Putri F. Studies on Isolation, Identification and Characterization of Alcohol Fermentative Yeasts Indigenous to Vegetable and Fruit Waste in Indonesia. Japan: Mie University; 2018.
  24. Birmeta G, Bakeeva A, Passoth V. Yeasts and bacteria associated with kocho, an Ethiopian fermented food produced from enset (Ensete ventricosum). Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology. 2019;112(4):651–659. DOI: https://doi.org/10.1007/s10482-018-1192-8.
  25. Vervaeke S, Vandamme K, Boone E, De Laere E, Swinne D, Surmont I. A case of Candida lambica fungemia misidentified as Candida krusei in an intravenous drug abuser. Medical Mycology. 2008;46(8):853–856. DOI: https://doi.org/10.1080/13693780802342552.
  26. Harding MW, Butler N, Dmytriw W, Rajput S, Burke DA, Howard RJ. Characterization of Microorganisms from Fresh Produce in Alberta, Canada Reveals Novel Food-spoilage Fungi. Research Journal of Microbiology. 2017;12(1):20–32. DOI: http://doi.org/10.3923/jm.2017.20.32.
  27. Vontrobova E, Kubizniakova P, Fiala J, Sochor J, Matoulkova D. Autochthonous yeasts as one of the tools to produce wines by original technologies. Kvasny Prumysl. 2019;65(1):38–45. DOI: https://doi.org/10.18832/kp2019.65.38.
  28. Bengoa AA, Iraporda C, Garrote GL, Abraham AG. Kefir micro-organisms: their role in grain assembly and health properties of fermented milk. Journal of Applied Microbiology. 2019;126(3):686–700. DOI: https://doi.org/10.1111/jam. 14107.
  29. Miao YJ, Xiong GT, Li RY, Wu ZF, Zhang X, Weng PF. Transcriptome profiling of Issatchenkia orientalis under ethanol stress. Amb Express. 2018;8. DOI: https://doi.org/10.1186/s13568-018-0568-5.
Как цитировать?
Indigenous yeast with cellulose-degrading activity in napa cabbage (Brassica pekinensis L.) waste: Characterisation and species identification. Foods and Raw Materials, 2019, vol. 7, no. 2, pp. 321-328
DOI
http://doi.org/10.21603/2308-4057-2019-2-321-328
Издатель
Кемеровский государственный университет
htpps://kemsu.ru
ISSN
2308-4057 (Print) /
2310-9599 (Online)
О журнале