Abstract

Metastable electrochemically-activated water solutions possess unique properties that make it possible to modify food emulsions. This comparative analysis featured the stability of model oil-in-water emulsions with anolyte or catholyte as a dispersion medium, as well as the physical and morphometric profile of the emulsion system. The research involved emulsions based on anolyte and catholyte. They consisted of refined sunflower oil, emulsifier (lecithin), and stabilizers, which were represented by sodium alginate, sodium carboxymethylcellulose, pectins, and agar. The study also covered such parameters as aggregative stability, viscosity, morphometry, oil particle size, and zeta potential. Anolyte and catholyte affected the process of separation in the model emulsions. The samples stabilized with alginate and sodium carboxymethylcellulose proved to be the most stable emulsions while agar triggered gelation. The effect of substituting tap water with metastable electrolyzed water solutions depended on the oil proportion in the emulsion. Catholyte destabilized the samples with 20% of oil and liquified gel in the samples stabilized with agar. Anolyte was more aggressive in destabilizing emulsions with 30% of oil. The effective viscosity of these emulsions correlated with the stable phase fraction. The anolytebased samples had low effective viscosity. The opposite results for emulsions with different oil fractions may have been caused by interface changes, i.e., surface tension, adsorption, coalescence, etc. In the emulsions with 46% of oil and animal origin emulsifier, neither anolyte nor catholyte had any significant effect on the aggregative stability of the system. The revealed patterns can be used to control the properties of emulsion products with oil phase ≤ 30%, e.g., low-fat mayonnaises, sauces, emulsion drinks, etc. Metastable electrolyzed water solutions may provide a reagent-free control of properties and patterns of finished or semi-finished foods and biological raw materials.

Keywords

Electrolyzed water, anolyte, catholyte, oil-in-water emulsion, aggregative stability, scanning electron microscopy

FUNDING

The research was supported by Russian Science Foundation (RSF) , grant No. 20-16-00019 (https://rscf.ru/en/ project/20-16-00019).

REFERENCES

1. Henry M, Chambron J. Physico-chemical, biological and therapeutic characteristics of electrolyzed reduced alkaline water (ERAW). Water. 2013;5(4):2094–2115. https://doi.org/10.3390/w5042094
2. Bakhir VM, Pogorelov AG. Universal electrochemical technology for environmental protection. International Journal of Pharmaceutical Research and Allied Sciences. 2018;7(1):41–57.
3. Ignatov I, Gluhchev G, Karadzhov S, Yaneva I, Valcheva N, Dinkov G et al. Dynamic nano clusters of water on waters catholyte and anolyte: Electrolysis with nano membranes. Physical Science International Journal. 2020;24(1):46–54. https://doi.org/10.9734/psij/2020/v24i130173
4. Ignatov I, Gluhchev G, Neshev N, Mehandjiev D. Structuring of water clusters depending on the energy of hydrogen bonds in electrochemically activated waters Anolyte and Catholyte. Bulgarian Chemical Communications. 2021;53(2):234–239.
5. Chen B-K, Wang C-K. Electrolyzed water and its pharmacological activities: A mini-review. Molecules. 2022;27(4):1222. https://doi.org/10.3390/molecules27041222
6. Rebezov M, Saeed K, Khaliq A, Rahman SJU, Sameed N, Semenova A, et al. Application of electrolyzed water in the food industry: A review. Applied Sciences. 2022;12(13):6639. https://doi.org/10.3390/app12136639
7. Li Y, Yang G, Yu S, Kang Z, Mo J, Han B, et al. In-situ investigation and modeling of electrochemical reactions with simultaneous oxygen and hydrogen microbubble evolutions in water electrolysis. International Journal of Hydrogen Energy. 2019;44(52):28283–28293. https://doi.org/10.1016/j.ijhydene.2019.09.044
8. Sipahi H, Reis R, Dinc O, Kavaz T, Dimoglo A, Aydın A. In vitro biocompatibility study approaches to evaluate the safety profile of electrolyzed water for skin and eye. Human and Experimental Toxicology. 2019;38(11):1314–1326. https://doi.org/10.1177/0960327119862333
9. Yurkevich AB. Dependence of physicochemical parameters of anolyte and catholyte on the concentration of initial aqueous solutions of sodium chloride. Pharmacy Bulletin. 2002;4:66–72. (In Russ.).
10. Iram A, Wang X, Demirci A. Electrolyzed oxidizing water and its applications as sanitation and cleaning agent. Food Engineering Reviews. 2021;13:411–427. https://doi.org/10.1007/s12393-021-09278-9
11. Pinton MB, dos Santos BA, Lorenzo JM, Cichoski AJ, Boeira CP, Campagnol PCB. Green technologies as a strategy to reduce NaCl and phosphate in meat products: An overview. Current Opinion in Food Science. 2021;40:1–5. https://doi.org/10.1016/j.cofs.2020.03.011
12. Sena Vaz Leães Y, Basso Pinton M, Terezinha de Aguiar Rosa C, Robalo SS, Wagner R, de Menezes CR, et al. Ultrasound and basic electrolyzed water: A green approach to reduce the technological defects caused by NaCl reduction in meat emulsions. Ultrasonics Sonochemistry. 2020;61:104830. https://doi.org/10.1016/j.ultsonch.2019.104830
13. Sena Vaz Leães Y, Silva JS, Robalo SS, Basso Pinton M, dos Santos SP, Wagner R, et al. Combined effect of ultrasound and basic electrolyzed water on the microbiological and oxidative profile of low-sodium mortadellas. International Journal of Food Microbiology. 2021;353:109310. https://doi.org/10.1016/j.ijfoodmicro.2021.109310
14. Li Y, Zeng Q-H, Liu G, Peng Z, Wang Y, Zhu Y, et al. Effects of ultrasound-assisted basic electrolyzed water (BEW) extraction on structural and functional properties of Antarctic krill (Euphausia superba) proteins. Ultrasonics Sonochemistry. 2021;71:105364. https://doi.org/10.1016/j.ultsonch.2020.105364
15. Hu W, Li P, Guo D, Zhang B, Tao D, Li J, et al. Effect of solution pulsed plasma process on the degradation and physicochemical properties of pectin. Food Hydrocolloids. 2023;136:108236. https://doi.org/10.1016/j.foodhyd.2022.108236
16. Zhang L, Lin W-F, Zhang Y, Tang C-H. New insights into the NaCl impact on emulsifying properties of globular proteins. Food Hydrocolloids. 2022;124:107342. https://doi.org/10.1016/j.foodhyd.2021.107342
17. Han F, Shen Q, Zheng W, Zuo J, Zhu X, Li J, et al. The conformational changes of bovine serum albumin at the air/water interface: HDX-MS and interfacial rheology analysis. Foods. 2023;12(8):1601. https://doi.org/10.3390/foods12081601
18. Banerjee A, De R, Das B. Hydrodynamic and conformational characterization of aqueous sodium alginate solutions with varying salinity. Carbohydrate Polymers. 2022;277:118855. https://doi.org/10.1016/j.carbpol.2021.118855
19. Król Ż, Malik M, Marycz K, Jarmoluk A. Characteristic of gelatine, carrageenan and sodium alginate hydrosols treated by direct electric current. Polymers. 2016;8(8):275. https://doi.org/10.3390/polym8080275
20. Yang Z, Yu S, Chen H, Guo X, Zhou J, Meng H. Effect of electrochemistry modification on the macromolecular, structural, and rheological characteristics of citrus peel pectin. Food Hydrocolloids. 2023;136:108246. https://doi.org/10.1016/j.foodhyd.2022.108246
21. Antony P, Bhat SS, Tallur PN, Naik VM. Effect of activity coefficient of polyvalent ionic salt solution on demulsification of soy lecithin based oil-in-water emulsion. Asian Journal of Chemical Sciences. 2019;6(1):1–11. https://doi.org/10.9734/ajocs/2019/v6i118988
22. Ito H, Kabayma S, Goto K. Effects of electrolyzed hydrogen water ingestion during endurance exercise in a heated environment on body fluid balance and exercise performance. Temperature. 2020;7(3):290–299. https://doi.org/10.1080/23328940.2020.1742056
23. Nagamatsu Y, Nagamatsu H, Ikeda H, Shimizu H. Microbicidal effect and storage stability of neutral HOCl-containing aqueous gels with different thickening/gelling agents. Dental Materials Journal 2021;40(6):1309–1319. https://doi.org/10.4012/dmj.2020-454
24. Bölek S, Tosya F, Dinç Ö. Effects of different types of electrolyzed waters on rheological characteristics of dough and quality properties of bread. Food Science and Technology International. 2023. https://doi.org/10.1177/10820132231170288
25. Gorbacheva MV, Tarasov VE, Kalmanovich SA, Sapozhnikova AI. Electrochemical activation as a fat rendering technology. Foods and Raw Materials. 2021;9(1):32–42. https://doi.org/10.21603/2308-4057-2021-1-32-42
26. Frolova YuV, Sobolev RV, Sarkisyan VA, Kochetkova AA. Effect of polysaccharide compounds on the stability of oil-in-water emulsions during storage. Food Processing: Techniques and Technology. 2022;52(1):32–45. (In Russ.). https://doi.org/10.21603/2074-9414-2022-1-32-45
27. Bredikhin SA, Martekha AN, Andreev VN, Kaverina YuE, Korotkiy IA. Rheological properties of mayonnaise with non-traditional ingredients. Food Processing: Techniques and Technology. 2022;52(4):739–749. (In Russ.). https://doi.org/10.21603/2074-9414-2022-4-2402
28. Abdullah, Liu L, Javed HU, Xiao J. Engineering emulsion gels as functional colloids emphasizing food applications: A review. Frontiers in Nutrition. 2022;9. https://doi.org/10.3389/fnut.2022.890188
29. Pandya YH, Bakshi M, Sharma A. Agar-agar extraction, structural properties and applications: A review. The Pharma Innovation Journal 2022;11(6):1151–1157.
30. Pogorelov AG, Ipatova LG, Pogorelova MA, Kuznetsov AL, Suvorov OA. Properties of serum albumin in electrolyzed water. Foods and Raw Materials. 2022;10(1):117–126. https://doi.org/10.21603/2308-4057-2022-1-117-126
31. Pogorelov AG, Ipatova LG, Panait AI, Pogorelova MA, Gulin AA, Pogorelova VN. Spectrometry of plant polysaccharides in electrochemically activated water. Modern Trends in Biological Physics and Chemistry. 2021;6(3):511–515. (In Russ.).
32. Kornena EP, Kalmanovich SA, Martovshchuk EV, Tereshchuk LV, Martovshchuk VI, Poznyakovskiy VM. Examining oils, fats, and their products. Quality and safety. Novosibirsk: Sibirskoye universitetskoye izdatel’stvo, 2017. 384 p.