ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Chemical composition of indigenous raw meats

Abstract
Using non-traditional raw meats is a promising direction of the meat industry. They include venison and meat from yak, elk, red deer, rabbits, ostriches, upland game, and musk-ox. The study aimed to compare the biochemical and physicochemical parameters of the meat from reindeer, elk, and musk-ox living in the Yenisei North. We found that these meats had a high biological value. First, we studied the composition of amino acids, fatty acids, vitamins, and minerals in the meat from reindeer of various sex and age groups. We found that the difference between the contents of unsaturated and saturated fatty acids and their ratio in adult and young reindeer males was in favour of young males, while these parameters did not differ in the meat from reindeer females of various age groups. Nevertheless, reindeer females had a higher content of saturated acids compared to reindeer male meat. Further, we studied the composition of amino acids, fatty acids, vitamins, and minerals in Taimyr elk and musk-ox meats. According to the results, the contents of essential amino acids in Taimyr elk and musk-ox meats were 60% and 55%, respectively. In both cases, the dominant amino acids were leucine, isoleucine, lysine, and valine. The study revealed the benefits of using indigenous meats in sausage production. Finally, we showed the influence of starter cultures on the quality of dry sausages and developed formulations of venison-based sausages.
Keywords
Meat industry , ungulate meat , protein quality index , amino acids , vitamins , macroelements , microelements , indigenous animals
INTRODUCTION

Numerous studies have shown that food production in Russia does not meet the physiological needs of Russian people, especially those in the Extreme North. The northern population, including children, has a severely unbalanced diet, which has been confirmed by extensive epidemiological studies and recent monitoring of nutritional status. Such food patterns result in an increased occurrence of nutritional diseases, lower efficiency, and reduced life expectancy, leading to unjustified social and economic losses. According to the WHO, nutrition accounts for 70% of people’s health and physical development [1].

Using local raw materials and creating an indigenous metabolic type of nutrition can be the fastest and most effective way to improve the diet and eliminate micronutrient deficiencies [2–5].

Enriching foods with functional ingredients is one of the promising directions in this area. Such ingredients can regulate the amount of essential substances in the human body [6, 7].

Of no less importance is the production of combined meat products from indigenous animals with the addition of plant materials with high consumer appeal [8–11].

STUDY OBJECTS AND METHODS

The objects of the study were meat samples derived from ungulates of various sex and age groups. Nutritional value indicators were determined as follows:

– moisture mass fraction: according to State Standard 23042-86;
– protein mass fraction: using the Kjeldahl method for nitrogen determination, State Standard 25011-81;
– mineral composition: by mass spectrometry;
– amino acid composition: using Hitachi L-8800 and Hitachi-835 amino acid analysers;
– fat content: using a Soxhlet apparatus, State Standard 23042-86;
– fatty acid composition: by gas-liquid chromatography; and
– vitamin content: by infrared spectroscopy.

Coliform bacteria were assayed according to State Standard R 52816-2007; Salmonella – according to State Standard R 50480-93. The microstructural indicators of meat were obtained using a Stereo Discovery V8 microscope. The 192 × magnified images were taken using a computer video system with Zeiss lenses.

RESULTS AND DISCUSSION

The first stage of the study focused on the amino acid, vitamin, and mineral composition of raw meats. The second stage aimed to develop a sausage technology.

Table 1 shows the amino acid composition of reindeer meat.

Table 1. Amino acid composition of reindeer meat by age and sex group

A high content of essential amino acids in reindeer meat makes it a balanced type of raw meat.

In the body, tryptophan is converted into biologically active compounds containing an indole ring (tryptamine, serotonin, and adrenochrome) and a pyridine ring (nicotinic acid, or vitamin PP). Tryptophan is used in treatment of heart disease to control body weight, suppress appetite, alleviate migraine attacks, and reduce the harmful effects of nicotine. Phenylalanine is oxidized to tyrosine. These amino acids are substrates for the synthesis of thyroxine, adrenaline, and noradrenaline. Phenylalanine is involved in the synthesis of collagen and connective tissue. It improves memory, attention, and circulation and contributes to the formation of insulin. Sulphur-containing amino acids are vital biologically active compounds. They are substrates for the synthesis of glutathione, insulin, lipoic acid, vitamin B1, and a number of enzymes.

The fatty acid, vitamin, and mineral compositions of reindeer meat are presented in Tables 2–4.

Table 2. Fatty acid composition of reindeer meat by age and sex group
Table 3. Vitamin content in reindeer meat by age and sex group
Table 4. Mineral content in reindeer meat by age and sex group

According Table 2, the meat of females had a higher content of saturated fatty acids than that of males. Unsaturated fatty acids were dominated by oleic acid, accounting for 65.3%.

The comparison of age and sex groups showed that the meat of female calves was richer in vitamins compared to male calves. The adult meats had a higher vitamin content compared to the young meats.

We noted that the content of macro- and microelements in reindeer meat increased with the age of the animals.

Elk meat is rich in phosphorus, potassium, and sodium. Phosphorus is part of organic compounds in phospholipids, nucleotides, and phosphoproteins. It is involved in the metabolism and maintains the acid-base balance in the body. Potassium and sodium are elements of the reticuloendothelial system present in the hydrated layer of bone tissue crystals. They play an important role in maintaining the osmotic pressure of the blood. Elk meat is also rich in iron, a vital part of haemoglobin. It promotes oxygen transfer from the lungs to tissues and performs a catalytic function, participating in redox reactions. In addition, elk meat has a rather high content of zinc and copper participating in many biochemical processes.

Tables 5–8 show the contents of amino acids, vitamins, and minerals, as well as the fatty acid composition of Taimyr elk meat.

Table 5. Amino acid composition of Taimyr elk meat
Table 6. Fatty acid composition of Taimyr elk meat
Table 7. Vitamin content in Taimyr elk meat
Table 8. AMineral content in Taimyr elk meat

We found that Taimyr elk meat had all essential amino acids in the amounts close to the standard. Arginine is a vital component of muscle tissue metabolism. It maintains the optimal nitrogen balance in the body, slows down tumour growth, and stimulates the immune system. The biochemical analysis of elk meat indicated its high biological value.

As seen from Table 6, the ratio of unsaturated to saturated fatty acids in elk meat was 1.58:1, which is indicative of the quality of intermuscular fat. Among unsaturated fatty acids, oleic acid was prevalent in all the samples, accounting for 84.9% of all unsaturated acids.

The results of the vitamin content analysis revealed that among group B vitamins, the highest concentrations were of pantothenic acid and cyanocobalamin.

The mineral composition of elk meat included a variety of minerals that are known to form chelate, ionic, and other biologically active complexes with functional properties. Elk meat contained 85.85% protein, 1.63% fat, and 4.84% ash elements. The calorie content in 100 g of meat was 358.07 kcal (1500 kJ).

We also determined the composition of amino acids, fatty acids, vitamins, and minerals in musk-ox meat. The results are presented in Tables 9–12.

Table 9. Amino acid composition of musk-ox meat
Table 10. Fatty acid composition of musk-ox meat
Table 11. Vitamin content in musk-ox
Table 12. Mineral content in musk-ox meat

According to the results, the content of essential amino acids in musk-ox meat was slightly higher than that of non-essential acids. Essential acids accounted for 54%.

Glutamine, alanine, and arginine prevailed among non-essential amino acids.

Tyrosine is a substrate for the synthesis of thyroxine, adrenaline, and noradrenalin. Methionine is a methyl group donor for the formation of many compounds, such as adrenaline, creatine, anserine, choline, and cysteine. In the body, cysteine turns into cysteamine, which mitigates the damaging effects of ionising radiation. Many enzymes owe their biological activity in the body to cysteine present in the molecule of sulfhydryl groups.

We calculated that the ratio of unsaturated to saturated fatty acids in musk-ox meat was about 1.66 : 1.

As seen from Table 10, oleic acid was prevalent in the meat, accounting for 85% of total unsaturated acids.

Musk-ox meat had quite a high content of fat-soluble vitamins, especially vitamin E. It was richer in vitamin B12 (125 μg/kg) than venison (27–29 μg/kg) or elk meat (30 μg/kg).

The analysis showed that musk-ox meat, in the same way as reindeer meat, had a high content of mineral elements such as phosphorus, potassium, and sodium. Of trace elements, musk-ox meat was rich in iron, which is known to be part of many protein and enzyme systems, involved in immunobiological processes. In addition, musk-ox meat had a relatively high content of zinc, which stimulates the immune system and protects the body from infections.

One of the criteria for evaluating a product’s nutritional value is the protein quality index (PQI) – a ratio of tryptophan to oxyproline. This index determines the ratio of muscle and connective tissue proteins. The PQI for ungulate meats is shown in Fig. 1.

Fig. 1. Protein quality index of meats, g/100 g of product.

As can be seen in Fig. 1, elk meat had the highest PQI compared to the other samples. It was probably due to nutrition patterns of this animal species.

At the next stage, we used the obtained data on the indigenous meats to develop a sausage technology.

Starter cultures were used in the production of dry smoked and cured sausages. Lactic acid bacteria, which are contained in starter cultures, ferment sugar and form lactic acid. The pH of the product decreases to the required level within 24–48 h, creating optimal conditions for sausage firming, reducing microbiological contamination, and rapid uniform drying.

The quality evaluation of the products included the following indicators: microbiological, sensory (appearance, slice colour, aroma, taste, texture, and overall evaluation), and physicochemical (pH, moisture content). We found that introducing a concentrate of starter cultures into coarsely cut meat during salting improves the sensory, colour, physicochemical, structural and mechanical, microstructural, and microbiological parameters of the products, increasing their biological activity.

The ripening of venison products is based on lactic acid bacteria that gradually become dominant and inhibit the development of undesirable microflora. The introduction of starter cultures doubled or even tripled the level of lactic acid microflora in the minced meat. In addition, using starter cultures eliminates the need for cooking meat at high temperatures, thus maintaining the quality of raw meat protein.

The microstructural analysis showed that starter cultures intensified the process of muscle tissue fermentation and, consequently, the structuring of the product. Thus, bacterial preparations can accelerate and stabilise the structural changes of the minced meat.

The pH value gradually decreased from 5.8 to 5.0–5.1 during the process. The moisture content decreased from 59.79 to 30%. Salt increased by 2.55–2.60%, remaining within the maximum permissible value. Smoked sausages were dried to 32–39% and cured sausages to 25–37%.

The decrease in moisture during the production of dry smoked sausages was accompanied by a significant reduction of the bacterial content in minced meat (from 2690 to 140–150 microbial cells in 1 g of product).

Table 13 gives a few examples of venison product formulations.

Table 13. Formulation of dry smoked and cured venison sausages
CONCLUSION

We studied a number of ungulate meats, namely reindeer, elk, and musk-ox meats. According to the results, all the meats contained a whole complex of biologically active substances, including essential unsaturated fatty acids, amino acids, vitamins, and minerals. In addition, the studied raw meats had a balanced combination of vital micronutrients. Therefore, we concluded that the meat of reindeer, elk, and muskox can be used to replenish their deficiency in the diet of people living in the Extreme North.

We also developed the formulation of dry smoked and cured venison sausages with desirable characteristics, including shelf life and sensory attributes.

REFERENCES
  1. Mediko-ehkologicheskiy monitoring sostoyaniya zdorovʹya naseleniya v zone promyshlennogo osvoeniya Severa [Medical and environmental monitoring of public health in the Northern industrial development zone] [Internet]. [cited 2019 Jun 20]. Available from: https://www.s-vfu.ru/universitet/rukovodstvo-i-struktura/instituty/mi/nauchnayarabota/ nir/monit/.
  2. Susʹ IV, Mittelʹshteyn TM, Antonova EN. Olenina-dopolnitelʹnyy istochnik syrʹya dlya myasnoy promyshlennosti [Venison is an additional source of raw materials for the meat industry]. All about the meat. 2012;(3):5–9. (In Russ.).
  3. Gorbunova NA, Nasonova VV. Nonconventional sources of meat raw materials of an animal origin. All about the meat. 2015;(5):46–51. (In Russ.).
  4. Kayzer AA, Shelepov VG. Tekhnologicheskaya skhema zagotovki i otsenki kachestva myasa i subproduktov kopytnykh zhivotnykh Eniseyskogo Severa [The process of procurement and quality assessment of meat and byproducts from ungulates in the Yenisei North]. Norilʹsk: Scientific Research Institute of Agriculture of the Extreme North, the Russian Academy of Agricultural Sciences; 2013. 110 p. (In Russ.).
  5. Yuzhakov AA. Kachestvennye pokazateli myasa severnykh oleney Arkticheskoy zony. Rossiya v novykh realiyakh mirovogo prodovolʹstvennogo rynka [Quality indicators of reindeer meat in the Arctic zone. Russia in the new realities of the world food market]. St. Petersburg-Pushkin: North-West Centre for Interdisciplinary Studies of Food Security Problems; 2016. 153–157 pp. (In Russ.).
  6. Kayshev VG, Seryogin SN. Sostoyanie i perspektivy razvitiya rynka funktsionalʹnykh produktov pitaniya [The state and development prospects of the functional food market]. Meat Industry. 2017;179(11):24–27. (In Russ.).
  7. Meleshchenya AV, Gordynets SA, Savelʹeva TA, Kaltovich IV. Osnovnye printsipy sozdaniya myasnykh produktov funktsionalʹnogo naznacheniya dlya pitaniya uchashcheysya molodezhi i lyudey, zanimayushchikhsya umstvennym trudom: monografiya [Basic principles of creating functional meat products for students and people in intellectual jobs]. Minsk: Institute of Meat and Dairy Industry; 2017. 159 p. (In Russ.).
  8. Utrilla MC, Soriano A, García Ruiz A. Determination of the optimal fat amount in dry-ripened venison sausage. Italian Journal of Food Science. 2015;27(4):409–415.
  9. Karwowska M, Dolatowski ZJ. Effect of acid whey and freeze-dried cranberries on lipid oxidation and fatty acid composition of nitrite-/nitrate-free fermented sausage made from deer meat. Asian-Australasian Journal of Animal Sciences. 2017;30(1):85–93. DOI: https://doi.org/10.5713/ajas.16.0023.
  10. Bogdan EG, Turshuk EG. Research of components used for developing recipes with meat of domesticated reindeer. Vestnik of MSTU. 2015;18(1):69–73. (In Russ.).
  11. Serba EM, Rimareva LV, Sokolova EN, Borshcheva YuA, Kurbatova EI, Volkova GS, et al. Biotechnological foundations of directed conversion of agricultural raw materials and secondary bioresources for obtaining food ingredients, functional food and feed. Moscow: BIBLIO-GLOBUS; 2017. 178 p. (In Russ.).
  12. Robbek NS, Barashkova AI, Reshetnikov AD, Rumyantseva TD, Savvin RG. The role of venison in nutrition of the North natives. Agrarian Bulletin of the Urals. 2015;139(9):25–31. (In Russ.).
  13. Krishtafovich VI, Poznyakovskiy VM, Goncharenko OA, Krishtafovich DV. ovarovedenie i ehkspertiza myasnykh i myasosoderzhashchikh produktov [Research and examination of meats and meat products]. St. Petersburg: Lan’; 2017. 432 p. (In Russ.).
How to quote?
Chemical composition of indigenous raw meats. Foods and Raw Materials, 2019, vol. 7, no. 2, pp. 412-418
DOI
http://doi.org/10.21603/2308-4057-2019-2-412-418
Publisher
Kemerovo State University
htpps://kemsu.ru
ISSN
2308-4057 (Print) /
2310-9599 (Online)
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