Аннотация

The screening of 8 probiotic strains (Propionibaсterium, Lactobacillus and Bifidobacterium) characterized by high cholesterol-lowering activity was carried out in the scientific research laboratory of East Siberia University of Technology and Management (Ulan-Ude, Russia). In order to research biotechnological potential of probiotic microorganisms and to conduct statistical analysis we used a mix of standard and modern physicochemical, biochemical and microbiological methods. The strains Lactobacillus helveticus 35-1, Propionibacterium shermanii AC-2503 and Bifidobacterium longum DK-100 were found out to have the highest cholesterol-lowering properties. It can be seen that cocultivation of Lactobacillus helveticus 35-1 and Propionibacterium shermanii AC-2503 strains shows high population density, cholesterol-metabolizing, antimutagenic, adhesive properties and exopolysaccharide biosynthesis. It indicates stable symbiotic bonds between cultures, which served as the basis for making a combined ferment. The analysis of biotechnological potential of Lactobacillus helveticus 35-1 and Propionibacterium shermanii АС-2503 strains and the study of organoleptic, physicochemical, probiotic properties and cholesterol metabolizing activity of the combined ferment have allowed to establish an optimum culture ratio in the ferment - 5 : 95. Optimum cultivation conditions of the continuous culture Lactobacillus helveticus 35-1 and the combined ferment were selected, process parameter of biologically active supplement and frozen ferments of direct loading were substantiated.

Ключевые слова

bifidobacteria, propionic bacteria, lactobacteria, cholesterol-lowering activity, exopolysaccharide, bacterial concentrate

СПИСОК ЛИТЕРАТУРЫ

1. Bokeriya L.A. and Oganova G.M. Vse o kholesterine: natsional'nyy doklad [Everything about cholesterol: a national report]. Moscow: NTsSSKh named after A. N. Bakulev RAMN Publ., 2010. 180 p.
2. Kuznetsov M.R. Diagnostika i lechenie lipidnogo distress-sindroma pri obliteriruyushchem ateroskleroze. Diss. dokt. med. nauk [Diagnosis and treatment of lipid distress syndrome with obliterating atherosclerosis. Dr. med. sci. diss.]. Moscow, 2000.
3. Bairaktari E.T., Tzallas C.S., Tsimihodimos V.K., Liberopoulos E.N., Miltiadous G.A., and Elisaf M.S. Comparison of the efficacy of atorvastatin and micronized fenofibrate in the treatment of mixed hyperlipidemia. J. Cardiovasc Risk, 1999, no. 6(2), pp. 113-136.
4. Ellerbe P., Myers G.L., Cooper G.R., et al. A comparison of results for cholesterol in human serum obtained by the Reference method and by the Definitive method of the National Reference System for cholesterol. Journal of Clin Chem., 1990, vol. 36. рр. 370-375.
5. Heaton K.W. The role of the large intestine in cholesterol gallstone formation. Bile acids in hepatobiliary diseases. Dordrecht: Kluwer Publ., 2000, рр. 192-199.
6. Shenderov B.A. Meditsinskaya mikrobnaya ekologiya i funktsional'noe pitanie. Tom I, II i III. [Medical microbial ecology and functional nutrition. Vols I-III]. Moscow: Grant Publ., 1998. 704 p.
7. Semenova E.V., Vorob'eva L.I., Netrusov A.I., Egorov N.S., and Yudina T.G. Metabolizm mikroorganizmov [The metabolism of microorganisms]. Moscow: MGU Publ., 1986. 256 p.
8. Haberer P., Du Toit M., Disk M.T., Ahrens F., and Holzapfel W.H. Effect of potentially probiotic lactobacillionfaecal enzyme activity in mini pigs on a high-fat high-cholesterol diet. A preliminary in vivo trial. Int. J. Food Microbiol, 2003, vol. 87, рр. 287-291.
9. Kumar R., Grover S., and Batish. Hypocholesterolaemic effect of dietary inclusion of two putative probiotic bile salt hydrolase-producing Lactobacillus plantarum strains in Sprague-Dawley rats. Br. J. Nutr., 2011, vol. 105, pp. 561-573.
10. Liu J.-R., Wang V.-Y., Chem V.-J., Chen H.-L., Yueh P.-Y., and Lin C.-W. Hypocholesterolaemic effects of milk-kefir and soy-amilk-kefir in cholesterol-fed hamsters. Br. J. Nutr., 2006, vol. 95, pp. 939-946.
11. Shenderov B.A. Funktsional'noe pitanie i ego rol' v profilaktike metabolicheskogo sindroma [Functional food and its role in prevention of metabolic syndrome]. Moscow: DeLi print Publ., 2008. 319 p.
12. Lion M.T. and Shah N.P. Bile salt deconjugation ability, bile salt hydrolase activity and cholesterol co-precipitation ability of lactobacilli strains. Int. dairy J., 2005, vol. 15, pp. 391-398.
13. Al-Sheraji S.H., Ismail A., Manap M.Y., Mustafa S., Yusof R.M., and Hassan F.A. Hypocholesterolaemic effects of yoghurt containing Bifidobacterium preudocatenulatum G4 or Bifidobacterium longum BB536. Food Chem., 2012, vol. 135, pp. 356-361.
14. Shenderov B.A. Probiotics and functional food. General and favorite issues. Food ingredients: raw materials and additives, 2005, no. 2, pp. 23-26. (In Russian).
15. Khamagaeva I.S. Effect of sodium nitrite on the stability and antimutagenic activity of probiotic microorganisms. Storage and processing of farm products, 2011, no. 10, pp. 60-63. (InRussian).
16. Huang Y., Wang X., Wang J., We F., Sui Y., Yang L., and Wang Z. Lactobacillus plantarum strains as potential probiotic cultures with cholesterol-lowering activity. Journal of Dairy Science, 2013, vol. 96, no. 5, pp. 2746-2753.
17. Chiu C.-H., Lu T.-Y., Tseng Y.-Y., and Pan T.-M. The effects of Lactobacillus-fermented milk on lipid metabolism in hamsters fed on high-cholesterol diet. Appl. Miccrobiol. Biotechnol., 2006, vol. 71, pp. 238-245.
18. Fortina M.G., Ricci G., Mora D., Parini C., and Manachini P.L. Specific identification of Lactobacillushelveticus by PCR with pepC, pepN and htrA targeted primers. FEMS Microbiol. Lett., 2001, no. 198, рр. 85-89.
19. Khamagaeva I.S., Kachanina L.M., and Tumurova S.M. Biotekhnologiya zakvasok propionovokislykh bakteriy [Biotechnology of starters of propionic acid bacteria]. Ulan-Ude: VSGTU Publ., 2006. 172 p.
20. Balyabina M.D., Slepysheva V.V., and Kozlov A.V. Methods for cholesterol determination. Hepatology, 2004, vol. 6, no. 6, pp. 21-39. (In Russian).
21. Kaplan L.A. and Pesce A.J. Clinical Chemistry: theory, analysis, and correlation. Mosby/Elsevier, 2002. 1476 р.
22. Sale F.O., Marchesini S., Fishman P.H. and Berra B. A sensitive enzymatic assay for determination of cholesterol in lipid extracts. Anal. Biochem., 1984, vol. 142, рр. 347-350.
23. Pirog T.P., Grinberg T.A., and MalashenkoYu.R. Protective function of exopolysaccharides synthesized by bacteria Acetobacter sp. Microbiology, 1997, vol. 66, no. 3, pp. 335-340. (In Russian).
24. Vorob'eva L.I. Anti-mutagenic properties of bacteria. Applied Biochemistry and Microbiology, 2002, vol. 38, no. 2, pp. 115-127. (InRussian).
25. Brilis V.I., Brilene T.A., Lentsner Kh.P., and Lentsner A.A. Methods of studying the process of adhesive micro-organisms. Laboratory, 1986, no. 4, pp. 210-212. (In Russian).
26. Lisitskaya N.V., Nikolaev I.V., Tor'kova A.A., Popov V.O., andKoroleva O.V. Analysis of the functional properties of biologically active substances on models cells (review). Applied Biochemistry and Microbiology, 2012, vol. 48, no. 6, pp. 581-598. (In Russian).
27. Vorob'eva L.I. Stressors, stress and survival of bacteria (review). Applied Biochemistry and Microbiology, 2004, vol. 40, no. 3, pp. 261-269. (In Russian).
28. Fooks L.J., Fuller R., and Gibson G.R. Prebiotics, probiotics and human gut microbiology. Inter. Dairy J., 2002, vol. 9, no. 1, рр. 53-61.
29. Alhaj O.A., Kanekanian A.D, Peters A.C., and Tatham A.S. Hypocholesterolaemic effect of Bifidobacterium animalis subsp. lactis (Bb 12) and trypsin casein hydrolysate. Food Chem., 2010, vol. 135, pp. 356-361.
30. Nikolaev Yu.A. Extracellular factors of bacteria to adapt to adverse environmental conditions. Applied Biochemistry and Microbiology, 2004, vol. 40, no. 4, pp. 387-397. (In Russian).
31. Ganbarov Kh.B. and Dzhafarov M.M. Antibacterial activity of Lactobacillus genus Lactobacillus. Dairy Industry, 2006, no. 8, p. 56. (In Russian).
32. Golod N.A., Loiko N.G., Mulyukin A.L., Neimagov A.L., Vorob'eva L.I., Suzina N.E., Shanenko E.F., Gal'chenko V.F., and El'-Registan G.I. Adaptation of lactic acid bacteria to adverse growth conditions. Microbiology, 2009, vol. 78, no. 3, pp. 317-335. (In Russian).
33. Bukharin O.V., Gintsburg A.L., Romanova Yu.M., and El'-Registan G.I. Mekhanizmy vyzhivaemosti bakteriy [Mechanisms of bacterial survival]. Moscow: Meditsina Publ., 2005. 268 p.
34. Magdanova L.A. and Golyasnaya N.V. Heterogeneity of how to adapt the properties of the bacterial population. Microbiology, 2013, vol. 82, no. 1, pp. 3-13. (In Russian).
35. Vorob'eva L.I., Khodzhaev E.Yu., Novikova T.M., and Chudinov E.N. Anti-stress effect cross extracellular metabolites of bacteria, archaea and yeast (review). Applied Biochemistry and Microbiology, 2013, vol. 49, no. 4, pp. 333-344. (In Russian).