Аннотация
Physical and psychological stress is part of modern life, which means that consumers need alimentary support to restore their functional reserves. This research aimed to develop a new functional snack bar with reliable adaptogenic properties based on secondary raw materials of dwarf cedar (Pinus pumila (Pall.) Regel).The main objects of the study were pine nut cake and microstrobiles – waste products of pine nut oil production and pollen harvesting. Additional components included dried sea buckthorn cake, flaxseed cake, and date paste. The nutritional and bioactive profiles were determined by standardized methods. The adaptogenic properties were studied on azathioprine immunosuppression in laboratory mice (Open Field and Porsolt Forced Swim Tests). The cytomorphological examination relied on standard methods.
The snack bar developed had a high content of dietary fiber, essential amino acids, polyunsaturated fatty acids, vitamins, minerals, and polyphenolics. When introduced into the diet of mice, it levelled the negative impact of azathioprine and restored the initial indicators of general physical activity, exploratory behavior, endurance, anxiety, and relative weight of the liver. It also helped the laboratory animals to recover the healthy morphofunctional state and weight of the major immune organs, i. e., thymus and spleen. The fact that the mice regained their nonspecific resistance during immunosuppression was apparently associated
with the antioxidant activity of the bioactive components in the experimental snack bar.
In this research, by-products of pine nut processing and pollen harvesting yielded a new functional product with reliable adaptogenic properties. Natural phytocomponents in mass-market products can facilitate consumers’ adaptability to adverse environment by improving resistance to various stresses.
Ключевые слова
Pine nut cake, Pinus pumila, microstrobiles, functional product, snack bar, adaptogenic properties, histomorphology, azathioprine, immunosuppression, miceСписок литературы
- Arapova OI. Adaptation phenomenon. Theoretical review. Psychology. Psychophysiology. 2023;16(3):111–122. (In Russ.) https://elibrary.ru/EYPQSA
- Lavin KM, Coen PM, Baptista LC, Bell MB, Drummer D, et al. State of knowledge on molecular adaptations to exercise in humans: Historical perspectives and future directions. Comprehensive Physiology. 2022;12(2):3193–3279. https://doi.org/10.1002/j.2040-4603.2022.tb00211.x
- Devyatov AA, Fedorova TN, Berezhnoy DS, Stvolinskii SL, Tutelyan VA. Mechanisms of neuroprotective action of hesperetin and carnosine in focal ischemia of the brain in rats. Bulletin of Experimental Biology and Medicine. 2020;169:242–245. https://doi.org/10.1007/s10517-020-04859-w
- Ryabokoneva LA, Sergeeva IYU, Anshukov AV, Permyakova LV. Phytoadaptogens as Functional Ingredients for Food Systems (Review). Agro-industrial complex of Russia. 2024;31(1):105–118. (In Russ.) https://doi.org/10.55934/2587-8824-2024-31-1-105-118
- Sokurenko MS, Solovieva NL, Bessonov VV, Mazo VK. Polyphenolic compounds of the stilbenoid class: Classification, representatives, content in plant raw materials, structural features, use in the food industry and pharmacy. Problems of Nutrition. 2019;88(1):17–25. (In Russ.) https://doi.org/10.24411/0042-8833-2019-10002
- Mathesius U. Flavonoid functions in plants and their interactions with other organisms. Plants. 2018;7(2):30. https://doi.org/10.3390/plants7020030
- Bobrysheva TN, Anisimov GS, Zolotoreva MS, Bobryshev DV, Budkevich RO, et al. Polyphenols as promising biologically active compounds. Problems of Nutrition. 2023;92(1):92–107. (In Russ.) https://doi.org/10.33029/0042-8833-2023-92-1-92-107
- Kornyakova VV, Badtieva VA, Balandin MYU. Exploiting dietary supplements with antioxidant properties for enhancing physical efficiency at the state of physical fatigue in sports. Problems of Nutrition. 2020;89(3):86–96. (In Russ.) https://elibrary.ru/OTZQZK
- Nikolic MV, Jakovljevic VL, Bradic JV, Tomovic MT, Petrovic BP, et al. Korean and Siberian pine: Review of chemical composition and pharmacological profile. Acta Poloniae Pharmaceutica. 2022;79(6):785–797. https://doi.org/10.32383/APPDR/161040
- Vasilyeva AG, Chirikova NK. Phenolic compounds of the needles of Pinus Pumila (Pall.) Regel growing in Yakutia. Arctic and Subarctic Natural Resources. 2021;26(3):136–143. (In Russ.) https://doi.org/10.31242/2618-9712-2021-26-3-136-143
- Rogachev AD, Salakhutdinov NF. Chemical composition of Pinus sibirica (Pinaceae). Chemistry & Biodiversity. 2015;12(1):1–53. https://doi.org/10.1002/cbdv.201300195
- Babich OO, Milent'eva IS, Ivanova SA, Pavsky VA, Kashirskikh EV, et al. The potential of pine nut as a component of sport nutrition. Foods and Raw Materials. 2017;5(2):170–177. https://doi.org/10.21603/2308-4057-2017-2-170-177
- Giro TM, Moiseeva NE, Mokretsov IV, Sukhov MA. designing a recipe for dry-cured sausages from the meat of fished animals. Bulletin of the South Ural State University Series Food and Biotechnology. 2025;13(2):52–63. (In Russ.) https://doi.org/10.14529/food250206
- Shpatov AV, Popov SA, Salnikova OI, Kukina TP, Shmidt EN, et al. Composition and bioactivity of lipophilic metabolites from needles and twigs of Korean and Siberian pines (Pinus koraiensis Siebold & Zucc. and Pinus sibirica Du Tour). Chemistry and Biodiversity. 2017;14(2):e1600203. https://doi.org/10.1002/cbdv.201600203
- Li H, Wang Z, Xu Y, Sun G. Pine polyphenols from Pinus koraiensis prevent injuries induced by gamma radiation in mice. PeerJ. 2016;4:e1870. https://doi.org/10.7717/peerj.1870
- Huang Y, Zhu X, Zhu Y, Wang Z. Pinus koraiensis polyphenols: Structural identification, in vitro antioxidant activity, immune function and inhibition of cancer cell proliferation. Food & Function. 2021;12(9):4176–4198. https://doi.org/10.1039/d0fo03347b
- Takala R, Ramji DP, Choy E. The beneficial effects of pine nuts and its major fatty acid, pinolenic acid, on inflammation and metabolic perturbations in inflammatory disorders. International Journal of Molecular Sciences. 2023;24(2):1171. https://doi.org/10.3390/ijms24021171
- Ramos PAB, Pereira C, Gomes AP, Neto RT, Almeida A, et al. Chemical characterisation, antioxidant and antibacterial activities of Pinus pinaster Ait. and Pinus pinea L. bark polar extracts: Prospecting forestry by-products as renewable sources of bioactive compounds. Applied Sciences. 2022;12(2):784. https://doi.org/10.3390/app12020784
- Peng X, Feng C, Wang X, Gu H, Li J, et al. Chemical composition and antioxidant activity of essential oils from barks of Pinus pumila using microwave-assisted hydrodistillation after screw extrusion treatment. Industrial Crops and Products. 2021;166:113489. https://doi.org/10.1016/j.indcrop.2021.113489
- Dziedziński M, Kobus-Cisowska J, Stachowiak B. Pinus species as prospective reserves of bioactive compounds with potential use in functional food–current state of knowledge. Plants. 2021;10(7):1306. https://doi.org/10.3390/plants10071306
- Prosekov AYu, Dyshlyuk LS, Milent'eva IS, Pavsky VA, Ivanova SI, et al. Study of the biofunctional properties of cedar pine oil with the use of in vitro testing cultures. Foods and Raw Materials. 2018;6(1):136–143. https://doi.org/10.21603/2308-4057-2018-1-136-143
- Khanturgaeva VA, Hamaganova IV. The analysis and research of the composition a protein-vitamins product from plant materials. Proceedings of the Voronezh State University of Engineering Technologies. 2022;84(1):49–57. (In Russ.) https://doi.org/10.20914/2310-1202-2022-1-49-57
- Erdyneeva SA, Shiretorova VG, Radnaeva LD. Pharmacognostic study of Pinus sylvestris L. microstrobils. Problems of biological, medical and pharmaceutical chemistry. 2022;25(9):34−39. https://doi.org/10.29296/25877313-2022-09-05
- Shiretorova VG, Erdyneeva SA, Radnaeva LD. Elemental composition of microstrobili and sprouts of Pinus sylvestris, Pinus sibirica and Pinus pumila. Proceedings of Universities. Applied Chemistry and Biotechnology. 2022;12(4):605–611. (In Russ.) https://doi.org/10.21285/2227-2925-2022-12-4-605-611
- Zhamsaranova SD, Shiretorova VG, Erdyneeva SA, Tykheev AA, Lebedeva SN. Assessment of the adaptogenic properties of herbal tea based on pine needles and microstrobiles. Food Systems. 2025;8(1):124–133. https://doi.org/10.21323/2618-9771-2025-8-1-124-133
- Asyakina LK, Loseva AI, Milentʹeva IS, Prosekov AYu, Minina VI. Prospects for using Siberian federal district plants in the production of functional food products. Bulletin of the South Ural State University. Series. Food and Biotechnology. 2022;10(4):5–17. (In Russ.)
- Piskunenko KR, Popov VG. Development of formulation and production technology for polyfunctional food ingredient with adaptogenic properties. Polzunovskiy vestnik. 2021;(3):141–146. (In Russ.) https://elibrary.ru/MVQIQH
- Kowalska H, Kowalska J, Ignaczak A, Masiarz E, Domian E, et al. Development of a high-fibre multigrain bar technology with the addition of curly kale. Molecules. 2021;26(13):3939. https://doi.org/10.3390/molecules26133939
- Haș IM, Vodnar DC, Bungau AF, Tarce AG, Tit DM, et al. Enhanced elderberry snack bars: A sensory, nutritional, and rheological evaluation. Foods. 2023;12(19):3544. https://doi.org/10.3390/foods12193544
- Bratosin BC, Martău GA, Ciont C, Ranga F, Simon E, et al. Nutritional and physico-chemical characteristics of innovative bars enriched with Aronia melanocarpa by-product powder. Applied Sciences. 2024;14(6):2338. https://doi.org/10.3390/app14062338
- AlJaloudi R, Al-Dabbas MM, Hamad HJ, Amara RA, Al-Bashabsheh Z, et al. Development and characterization of high-energy protein bars with enhanced antioxidant, chemical, nutritional, physical, and sensory properties. Foods. 2024;13(2):259. https://doi.org/10.3390/foods13020259
- Cavalcanti ICA, Borges LA, de Carvalho MD, Pereira GSL, Oliveira MLP, et al. Development and evaluation of nutrient-rich food bars incorporating macauba kernel cake. Journal of Food Science and Technology. 2025;62:2191–2198. https://doi.org/10.1007/s13197-024-06166-y
- Khanturgaev AG, Shiretorova VG, Kotova TI, Khanturgaeva VA. Production of functional products from Siberian pine seeds: Scientific basis and practical implementation: monograph. Ulan-Ude: ESSUTM; 2018, 140 p. (In Russ.)
- Bakumenko OE, Ruban NV, Alekseenko EV. Scientific and practical aspects of developing snack bars for healthy nutrition. Kursk: University Book; 2023, 190 p. (In Russ.) https://doi.org/10.47581/2023/Bakumenko-Ruban.01
- Breil C, Vian MA, Zemb T, Kunz W, Chemat F. “Bligh and Dyer” and Folch methods for solid–liquid–liquid extraction of lipids from microorganisms. Comprehension of Solvatation mechanisms and towards substitution with alternative solvents. International Journal of Molecular Sciences. 2017;18(4):708. https://doi.org/10.3390/ijms18040708
- Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in enzymology. 1999;299:152–178. https://doi.org/10.1016/S0076-6879(99)99017-1
- Belyakov VI, Gromova DS, Popova NR, Myakisheva YuV. Modern methods for studying rodent behavior in model biomedical studies (problem review). Modern issues of Biomedicine. 2022;6(4):13–22. (In Russ.) https://doi.org/10.51871/2588-0500_2022_06_04_1
- Buslovich SYu, Kotelenets AI, Fridlyand RM. Integral method for assessing the behavior of white rats in an open field. Journal of Higher Nervous Activity. 1989;39(1):168–170. (In Russ.)
- Porsolt RD, Pichon ML, Jalfe M. Depression: A new animal model sensitive to antidepressant treatment. Nature. 1977;266:730–732. https://doi.org/10.1038/266730a0
- Korzhevskiy DE, Gilyarov AV. Fundamentals of histological technique. St. Petersburg: SpetsLit; 2010, 95 p. (In Russ.)
- Rațu RN, Veleșcu ID, Stoica F, Usturoi A, Arsenoaia VN, et al. Application of agri-food by-products in the food industry. Agriculture. 2023;13(8):1559. https://doi.org/10.3390/agriculture13081559
- Chen Y, Cai Y, Wang K, Wang Y. Bioactive compounds in sea buckthorn and their efficacy in preventing and treating metabolic syndrome. Foods. 2023;12(10):1985. https://doi.org/10.3390/foods12101985
- Wang Z, Zhao F, Wei P, Chai X, Hou G, et al. Phytochemistry, health benefits, and food applications of sea buckthorn (Hippophae rhamnoides L.): A comprehensive review. Frontiers in Nutrition. 2022;(9):1036295. https://doi.org/10.3389/fnut.2022.1036295
- Noreen S, Tufail T, Ul Ain HB, Awuchi CG. Pharmacological, nutraceutical, and nutritional properties of flaxseed (Linum usitatissimum): An insight into its functionality and disease mitigation. Food Science & Nutrition. 2023;11(11):6820–6829. https://doi.org/10.1002/fsn3.3662
- Niharika M, Sireesha G, Madhavi D, Sumalatha SV. Development of super food nutri rich bar. Journal of Health Sciences. 2021;8(4):254–259. https://doi.org/10.21276/apjhs.2021.8.4.25
- Ayad AA, Williams LL, Gad El-Rab DA, Ayivi R, Colleran HL, at al. A review of the chemical composition, nutritional and health benefits of dates for their potential use in energy nutrition bars for athletes. Cogent Food & Agriculture. 2020;6(1):1809309. https://doi.org/10.1080/23311932.2020.1809309
- Al-Karmadi A, Okoh AI. An overview of date (Phoenix dactylifera) fruits as an important global food resource. Foods. 2024;13(7):1024. https://doi.org/10.3390/foods13071024
- Alfheeaid HA, Barakat H, Althwab SA, Musa KH, Malkova D. Nutritional and physicochemical characteristics of innovative high energy and protein fruit- and date-based bars. Foods. 2023;12(14):2777. https://doi.org/10.3390/foods12142777
- Fernández-Ríos A, Laso J, Hoehn D, Amo-Setién FJ, Abajas-Bustillo R, et al. A critical review of superfoods from a holistic nutritional and environmental approach. Journal of Cleaner Production. 2022;379(Part 1):134491. https://doi.org/10.1016/j.jclepro.2022.134491
- Tsyrenova DZ, Gulyaev SM, Khobrakova VB. Effect of Phlomoides tuberosa (L.) Moench extract on spleen structure in immunosuppressed mice. Reviews in Clinical Pharmacology and Drug Therapy. 2017;15(1):53–57. (In Russ.) https://doi.org/10.17816/RCF15153-57
- Khobrakova VB, Razuvaeva YG, Budaeva ER. Correction of structural changes in thymus by Gentiana algida pall extract at experimental azathioprine immunosuppression. Antibiotics and Chemotherapy. 2020;65(7–8):18–22. (In Russ.) https://doi.org/10.37489/0235-2990-2020-65-7-8-18-22
- Lyubitelev A V, Sivkina AL, Vlasova O A, Belitsky GA, Studitsky VM. Mechanisms of action of plant polyphenols on the initiation of carcinogenesis. Advances in Molecular Oncology. 2023;10(2):30–41. (In Russ.) https://doi.org/10.17650/2313-805X-2023-10-2-30-41
- Tavan M, Hanachi P, de la Luz Cádiz-Gurrea M, Carretero AS, Mirjalili MH. Natural phenolic compounds with neuroprotective effects. Neurochemical Research. 2024;49(2):306–326. https://doi.org/10.1007/s11064-023-04046-z
- Quesada-Vázquez S, Eseberri I, Les F, Pérez-Matute P, Herranz-López M, et al. Polyphenols and metabolism: From present knowledge to future challenges. Journal of Physiology and Biochemistry. 2024;80(3):603–625. https://doi.org/10.1007/s13105-024-01046-7
- Chernukha IM, Polishchuk EK, Trubina MV, Nesterova MD, Kupaeva NV. Animal and plant by-products as a source of valuable biologically active substances: A review. Vse o myase. 2025;(1):48–57. (In Russ.) https://doi.org/10.21323/2071-2499-2025-1-48-57
- Mihaylova D, Dimitrova-Dimova M, Popova A. Dietary phenolic compounds–Wellbeing and perspective applications. International Journal of Molecular Sciences. 2024;25(9):4769. https://doi.org/10.3390/ijms25094769
- Burakova LN, Shkolnikova MN, Plotnikov DA. Composition and technology development of a complex adaptogenic food additive for the arctic zone population of the Russian Federation. Food Industry. 2023;8(1):32–42. https://doi.org/10.29141/2500-1922-2023-8-1-4
- Agarkova EYu, Belyakova ZYu, Kondratenko VV. Principles of formation of modular technologies of enteral nutrition products. Food Metaengineering. 2023;1(3):33–46. (In Russ.) https://doi.org/10.37442/fme.2023.3.26
