ISSN 2308-4057 (Печать),
ISSN 2310-9599 (Онлайн)

Red wines from the Mostar area: Physicochemical, antioxidative, and antimicrobial properties

Аннотация
Wines are complex alcoholic beverages. Apart from alcohol, they also contain other compounds, including those that have a beneficial effect on human health.
This paper features the basic physicochemical properties of four red grape varieties (Blatina, Vranac, Cabernet Sauvignon, Merlot) from the Mostar area, Bosnia and Herzegovina, as well as the antioxidant and antimicrobial properties of wines made of these grape varieties. The wines were produced in a standard way; the results were observed during two consecutive seasons of 2020 and 2021. The physicochemical properties were analyzed by standard methods recommended by the International Organization of Vine and Wine. The study involved tests for total phenolics, flavonoids, and anthocyanins, as well as for antioxidant activity. The methodology included FRAP, DPPH, and ABTS assays. The antimicrobial activity was tested by agar dilution method, which made it possible to determine the minimum inhibitory and bactericidal values. The list of pathogenic and opportunistic bacteria consisted of Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, and Bacillus cereus. Pathogenic yeasts were represented by Candida albicans. Lactobacillus plantarum and Saccharomyces boulardii were selected as probiotic cultures.
The physicochemical characteristics of grapes, i.e. must, depended on the harvest year, variety, and their interaction. The best antioxidant effect and the highest total phenolic content belonged to the Vranac wine, vintage 2020. B. cereus appeared to be the most sensitive bacteria. The Blatina wines of both harvest years demonstrated the lowest antimicrobial and the antioxidant activities. Probiotic cultures proved to be resistant to the effects of wine. The Pearson’s test revealed a reliable correlation between the antioxidant properties and the antimicrobial effect on B. cereus and, in one case, on S. aureus and P. aeruginosa.
All grapevine varieties in this research proved to be suitable for the production of quality wines in the Mostar area.
Ключевые слова
Red grape varieties, wine, must, physicochemical properties, antioxidant activity, antimicrobial activity
ФИНАНСИРОВАНИЕ
This work was partially supported by the Ministry of Scientific and Technological Development, Higher Education and Information Society of the Republic of Srpska (contract number: 19.032/961-70/19, December 31, 2019).
СПИСОК ЛИТЕРАТУРЫ
  1. Jovanović-Cvetković T, Sredojević M, Natić M, Grbić R, Akšić MF, Ercisli S, et al. Exploration and comparison of the behavior of some indigenous and international varieties (Vitis vinifera L.) grown in climatic conditions of Herzegovina: The influence of variety and vintage on physico-chemical characteristics of grapes. Plants. 2023;12(4):695. https://doi.org/10.3390/plants12040695
  2. Gnilomedova NV, Anikina NS, Kolesnov AYu. A review of methodological approaches to authenticating the geographical origin of wines. Food Processing: Techniques and Technology. 2023;53(2):231–246. (In Russ.). https://doi.org/10.21603/2074-9414-2023-2-242; https://elibrary.ru/AUQCPD
  3. Gutiérrez-Escobar R, Aliaño-González MJ, Cantos-Villar E. Wine polyphenol content and its influence on wine quality and properties: A review. Molecules. 2021;26(3):718. https://doi.org/10.3390/molecules26030718
  4. Kubyshkin A, Ogai Y, Fomochkina I, Chernousova I, Zaitsev G, Shramko Yu. Polyphenols of red grape wines and alcohol-free food concentrates in rehabilitation technologies. In: Wong J, editor. Polyphenols. IntechOpen; 2018. pp. 99–120. https://doi.org/10.5772/intechopen.76655
  5. Kesić A, Smajlovic B, Ibrišimović Mehmedinović N, Hodzic Z, Šestan A, Dedić J, et al. The content of total polyphenols of the selected red wines from the territory of Tuzla canton in correlation with antioxidant activity. International Journal of Research Methodology. 2019;2(1):141–151.
  6. Radonjić S, Maraš V, Košmerl T. The importance of total polyphenols content in red wine. Third International Mediterranean Congress on Natural Sciences, Health Sciences and Engineering; 2019; Podgorica. Podgorica; 2019. p. 231–240.
  7. Kauffmann AC, Castro VS. Phenolic compounds in bacterial inactivation: A perspective from Brazil. Antibiotics. 2023;12(4):645. https://doi.org/10.3390/antibiotics12040645
  8. Santoro HC, Skroza D, Dugandžić A, Boban M, Šimat V. Antimicrobial activity of selected red and white wines against Escherichia coli: In vitro inhibition using fish as food matrix. Foods. 2020;9(7):936. https://doi.org/10.3390/foods9070936
  9. Radovanović AN, Jovančičević BS, Radovanović BC, Mihajilov-Krstev T. Antimicrobial effectiveness of selected Vranac wines against six gram-positive and six gram-negative bacterial strains. Tropical Journal of Pharmaceutical Research. 2014;13(5):819–824. https://doi.org/10.4314/tjpr.v13i5.24
  10. Boban N, Tonkić M, Budimir D, Modun D, Sutlović D, Punda-Polic V, et al. Antimicrobial effects of wine: Separating the role of polyphenols, pH, ethanol, and other wine components. Journal of Food Science. 2010;75(5):M322–M326. https://doi.org/10.1111/j.1750-3841.2010.01622.x
  11. Milutinović M, Dimitrijevic-Branković S, Rajilić-Stojanović M. Plant extracts rich in polyphenols as potent modulators in the growth of probiotic and pathogenic intestinal microorganisms. Frontiers in Nutrition. 2021;8:688843. https://doi.org/10.3389/fnut.2021.688843
  12. The climate of Bosnia and Herzegovina [Internet]. [cited 2023 Oct 10]. Available from: https://www.fhmzbih.gov.ba/latinica/KLIMA/klimaBIH.php
  13. Federal hydrometeorological institute of Bosnia and Herzegovina [Internet]. [cited 2023 Oct 10]. Available from: http://www.fhmzbih.gov.ba
  14. Compendium of international methods of wine and must analysis. Paris; 2021. 673 p.
  15. Kupina S, Fields C, Roman MC, Brunelle SL. Determination of total phenolic content using the Folin-C assay: Single-laboratory validation, first action 2017.13. Journal of AOAC International. 2018;101(5):1466–1472. https://doi.org/10.5740/jaoacint.18-0031
  16. Correa Uriburu FM, Cattaneo F, Maldonado LM, Zampini IC, Alberto MR, Isla MI. Prosopis alba seed as a functional food waste for food formulation enrichment. Foods. 2022;11(18):2857. https://doi.org/10.3390/foods11182857
  17. Rodríguez-Vaquero MJ, Vallejo CV, Aredes-Fernández PA. Antibacterial, antioxidant and antihypertensive properties of polyphenols from Argentinean red wines varieties. Open Journal of Pharmacology and Pharmacotherapeutics. 2020;5(1):001–006. https://doi.org/10.17352/ojpp.000010
  18. Rumpf J, Burger R, Schulze M. Statistical evaluation of DPPH, ABTS, FRAP, and Folin-Ciocalteu assays to assess the antioxidant capacity of lignins. International Journal of Biological Macromolecules. 2023;233:123470. https://doi.org/10.1016/j.ijbiomac.2023.123470
  19. Mitrevska K, Grigorakis S, Loupassaki S, Calokerinos AC. Antioxidant activity and polyphenolic content of North Macedonian wines. Applied Sciences. 2020;10(6):2010. https://doi.org/10.3390/app10062010
  20. Kowalska-Krochmal B, Dudek-Wicher R. The minimum inhibitory concentration of antibiotics: Methods, interpretation, clinical relevance. Pathogens. 2021;10(2):165. https://doi.org/10.3390/pathogens10020165
  21. Geng D-H, Liu L, Zhou S, Sun X, Wang L, Zhou X, et al. Effects of Lactobacillus plantarum inoculum on the fermentation rate and rice noodle quality. Journal of Oleo Science. 2020;69(9):1031–1041. https://doi.org/10.5650/jos.ess20003
  22. Chelliah R, Kim E-J, Daliri EB-M, Antony U, Oh D-H. In vitro probiotic evaluation of Saccharomyces boulardii with antimicrobial spectrum in a Caenorhabditis elegans model. Foods. 2021;10(6):1428. https://doi.org/10.3390/foods10061428
  23. Catalogue of grapevines cultivated in France [Internet]. [cited 2023 Oct 15]. Available from: https://www.plantgrape.fr/en
  24. Ivanišević D, Kalajdžić M, Drenjančević M, Puškaš V, Korać N. The impact of cluster thinning and leaf removal timing on the grape quality and concentration of monomeric anthocyanins in Cabernet-Sauvignon and Probus (Vitis vinifera L.) wines. OENO One. 2020;54(1):63–74. https://doi.org/10.20870/oeno-one.2020.54.1.2505
  25. Aleinikova N, Didenko P, Shaporenko V, Didenko L, Belash S. The effect of mineral nutrition systems as an element of cultivation technology of wine grape varieties on their productivity in the conditions of Crimea. IOP Conference Series: Earth and Environmental Science. 2023;1206:012026. https://doi.org/10.1088/1755-1315/1206/1/012026
  26. Arestovа N, Ryabchun I. Influence of biostimulants on productivity and quality of grapes. E3S Web of Conferences. 2020;210:05001. https://doi.org/10.1051/e3sconf/202021005001
  27. Jovanovic-Cvetkovic T, Micic N, Djuric, G, Cvetkovic M. Pollen morphology and germination of indigenous grapevine cultivars Žilavka and Blatina (Vitis vinifera L.). AgroLife Scientific Journal. 2016;5(1):105–109.
  28. Jovanović-Cvetković T, Šutalo V, Kupe M, Ercisli S, Životić A, Pašalić B. Influence of interaction effects of the different pollenizers on the Blatina variety (Vitis vinifera L.) grape cluster and seed characteristics. Plants. 2022;11(3):420. https://doi.org/10.3390/plants11030420
  29. Banjanin T. Characterization of quantitative and qualitative characteristics of Blatina vine variety in agroecological conditions of Trebinje. Ph.D. Thesis. Belgrade; 2022.
  30. Maras V, Tomic M, Kodzulovic V, Knezevic B, Raicevic D, Cizmovic M. Yield and quality of grapes and wine of the cultivars “Vranac”, “Primitivo” and “NegroAmaro”. Acta Horticulturae. 2012;931. https://doi.org/10.17660/ActaHortic.2012.931.43
  31. Popović T, Raičević D. Yield and quality of grapes of autochthonous variety Vranac in agroecological conditions of Podgorica subregion. In: Jacimovic M, editor. The First International Conference on Vranac and Other Montenegrin Indigenous Sorts of Grapevine. Podgorica: Montenegrin Academy of Sciences and Art; 2021. pp. 197–206.
  32. Gombau J, Pons-Mercadé P, Conde M, Asbiro L, Pascual L, Gómez-Alonso S, et al. Influence of grape seeds on wine composition and astringency of Tempranillo, Garnacha, Merlot and Cabernet Sauvignon wines. Food Science and Nutrition. 2020;8:3442–3455. https://doi.org/10.1002/fsn3.1627
  33. Bubola M, Rossi S, Váczy KZ, Hegyi ÁI, Persic M, Zdunić G, et al. Modification of cv. Merlot berry composition and wine sensory characteristics by different leaf area to fruit ratios. Applied Sciences. 2023;13(9):5456. https://doi.org/10.3390/app13095465
  34. Liu H-F, Wu B-H, Fan P-G, Li S-H, Li L-S. Sugar and acid concentrations in 98 grape cultivars analyzed by principal component analysis. Journal of the Science of Food and Agriculture. 2006;86:1526–1536. https://doi.org/10.1002/jsfa.2541
  35. Zhong H, Yadav V, Wen Z, Zhou X, Wang M, Han S, et al. Comprehensive metabolomics based analysis of sugar composition and content in berries of 18 grape varieties. Frontiers in Plant Science. 2023;14:1200071. https://doi.org/10.3389/fpls.2023.1200071
  36. Coombe BG. Influence of temperature on composition and quality of grapes. Acta Horticulturae. 1987;206. https://doi.org/10.17660/ActaHortic.1987.206.1
  37. Banjanin T, Lisov N, Petrović A, Ranković-Vasić Z, Blesić M. The quality of grape and wine of Merlot and Blatina varieties in the agroecological conditions of the Trebinje vineyard. VIII International Symposium on Agricultural Sciences. Banja Luka: Faculty of Agriculture University of Banja Luka; 2019. p. 69–75.
  38. Jovanović-Cvetković T, Grbić R, Grobelnik Mlakar S, Bosančić B, Cvetković M. Physicochemical evaluation of the grape and wine of the Blatina, Trnjak and Vranac in different vintage. AgroLife Scientifc Journal. 2023;12(1):105–115. https://doi.org/10.17930/AGL2023113
  39. Maraš V, Kodžulović V, Mugoša M, Raičević J, Gazivoda A, Šućur S, et al. Clonal selection of autochthonous grape variety Vranac in Montenegro. In: Badnjevic A, editor. Proceedings of the International Conference on Medical and Biological Engineering 2017. Singapore: Springer; 2017. pp. 787–790. https://doi.org/10.1007/978-981-10-4166-2_118
  40. Sivcev B, Rankovic-Vasic Z, Petrovic A, Jancis R, Milisic K. Fruit characteristics of the Merlot clones in Belgrade wine Growing Region, Serbia. Journal of Advancements in Plant Science. 2018;1(2).
  41. Tecchio MA, da Silva MJR, Sanchez CAPC, Callili D, Vedoato BTF, Hernandes JL, et al. Yield performance and quality of wine grapes (Vitis vinifera) grafted onto different rootstocks under subtropical conditions. Crop Production and Management. 2022;81:e1622. https://doi.org/10.1590/1678-4499.20210214
  42. Yang J, Lee J. Current research related towine sensory perception since 2010. Beverages. 2020;6(3):47. https://doi.org/10.3390/beverages6030047
  43. Zoecklein BW, Fugelsang KC, Gump BH, Nury FS. Alcohol and extract wine. In: Zoecklein B, Fugelsang KC, Gump BH, Nury FS, editors. Wine analysis and production. Springer; 1999. pp. 97–113.
  44. Lima MMM, Choy YY , Tran J, Lydon M, Runnebaum RC. Organic acids characterization: Wines of Pinot noir and juices of “Bordeaux grape varieties”. Journal of Food Composition and Analysis. 2022;114:104745. https://doi.org/10.1016/j.jfca.2022.104745
  45. Vicente J, Baran Y, Navascués E, Santos A, Calderón F, Marquina D, et al. Biological management of acidity in wine industry: A review. International Journal of Food Microbiology. 2022;375:109726. https://doi.org/10.1016/j.ijfoodmicro.2022.109726
  46. International Code of Oenological Practices. Paris; 2022. 393 p. (In French).
  47. Riberéau-Gayon P, Dubordieu D, Donèche B, Lonvaud A. Treatise on oenology – Microbiology of wine, Vinifications. Paris: Dunod; 2004. 498 p. (In French).
  48. Paramithiotis S, Stasinou V, Tzamourani A, Kotseridis Y, Dimopoulou M. Malolactic fermentation – Theoretical advances and practical considerations. Fermentation. 2022;8(10):521. https://doi.org/10.3390/fermentation8100521
  49. Herjavec S, Jeromel A, Maslov L, Jagatić Korenika AM, Mihaljević M, Prusina T. Influence of different maceration times on the anthocyanin composition and sensory properties of blatina wines. Agriculturae Conspectus Scientificus. 2012;77(1):41–44.
  50. Lavrić M, Prusina T. The influence of the vintage year on Blatina wine quality. Proceedings of 55th Croatian & 15th International Symposium on Agriculture; 2020; Vodice. Zagreb: University of Zagreb; 2020. p. 501–504.
  51. Jagatić Korenika A-M, Tomaz I, Preinar D, Lavrić M, Šimić B, Jeremol A. Influence of L. thermotolerans and S. cerevisiae commercial yeast sequential inoculation on aroma composition of red wines (Cv Trnjak, Babic, Blatina and Frankovka). Fermentation. 2021;7(4):4. https://doi.org/10.3390/fermentation7010004
  52. Gašović B, Đaković J, Radonjić S, Maraš V, Kodžulović V. Characteristics and quality of grapes and wines of the Vranac variety. In: Jacimovic M, editor. The First International Conference on Vranac and Other Montenegrin Indigenous Sorts of Grapevine. Podgorica: Montenegrin Academy of Sciences and Art; 2021. pp. 267–277.
  53. Jakšić D, la Notte P, Giannini PB, Perović V, Cagnazzo A. Montenegrin Vranac vine variety in the most eastern point of the Vranac cultivation area – Knjaževac wine-growing region (Serbia). In: Jacimovic M, editor. The First International Conference on Vranac and Other Montenegrin Indigenous Sorts of Grapevine. Podgorica: Montenegrin Academy of Sciences and Art; 2021. pp. 207–225.
  54. Sošić S, Pajović-Šćepanović R, Raičević D, Popović T. Quality of wines Vranac and Kratošija in the vintage 2021. Agriculture and Forestry. 2023;69(1):127–137. https://doi.org/10.17707/AgricultForest.69.1.11
  55. Ivanova-Petropulos V, Ricci A, Nedelkovski D, Dimovska V, Parpinello GP, Versari A. Targeted analysis of bioactive phenolic compounds and antioxidant activity of Macedonian red wines. Food Chemistry. 2015;171:412–420. https://doi.org/10.1016/j.foodchem.2014.09.014
  56. Raičević D, Mijović S, Popović T, Pajović-Šćepanović R. The influence of variety and vintage on the chemical composition and sensory properties of red wines in Podgorica subregion (Montenegro). Journal of Agricultural, Food and Environmental Sciences. 2017;71(1):157–164.
  57. Eder R, Pajović Šćepanović R, Raičević D, Popović T, Korntheuer K, Wendelin S, et al. Study of the effects of climatic conditions on the phenolic content and antioxidant activity of Austrian and Montenegrin red wines. OENO One. 2023;57(3):69–85. https://doi.org/10.20870/oeno-one.2023.57.3.7450
  58. Xu S, Zhu J, Zhao Q, Gao J, Zhang H, Hu B. Quality evaluation of Cabernet Sauvignon wines in different vintages by 1H nuclear magnetic resonance-based metabolomics. Open Chemistry. 2021;19(1):385–399. https://doi.org/10.1515/chem-2020-0126
  59. Antonenko MV, Guguchkina TI, Prakh AV, Kolesnov AYu, Zenina MA. Research of physical and chemical characteristics of grapes from different regions of Krasnodar territory for their use as standards of authenticity of wine production. Fruit Growing and Viticulture of South Russia. 2019;55(1):95–106. https://doi.org/10.30679/2219-5335-2019-1-55-95-106
  60. Banjanin T, Ranković-Vasić Z, Nikolić D, Anđelić B. Influence of climatic factors on the quality of Merlot grapevine variety in Trebinje region vineyards (Bosnia and Herzegovina). Agrofor. 2019;4(2):95–101. https://doi.org/10.7251/AGRENG1902094B
  61. Miele A. Wine composition of Merlot and Cabernet Sauvignon vine clones under the environmental conditions of Serra Gaúcha, Brazil. Food Science and Technology. 2021;41(Suppl.1):116–122. https://doi.org/10.1590/fst.10520
  62. Muñoz-Bernal ÓA, Vazquez-Flores AA, de la Rosa LA, Rodrigo-García J, Martínez-Ruiz NR, Alvarez-Parrilla E. Enriched red wine: Phenolic profile, sensory evaluation and in vitro bioaccessibility of phenolic compounds. Foods. 2023;12(6):1194. https://doi.org/10.3390/foods12061194
  63. Pajović Šćepanović R, Madžgalj V, Vukoslavljević V. Assay of polyphenolic in Montenegrin Vranac wines. Mitteilungen Klosterneuburg. 2019;69:65–75.
  64. Pajović Šćepanović R, Wendelin S, Raičević D, Eder R. Characterization of the phenolic profile of commercial Montenegrin red and white wines. European Food Research and Technology. 2019;245:2233–2245. https://doi.org/10.1007/s00217-019-03330-z
  65. Tzanova M, Atanassova S, Atanasov V, Grozeva N. Content of polyphenolic compounds and antioxidant potential of some Bulgarian red grape varieties and red wines, determined by HPLC, UV, and NIR spectroscopy. Agriculture. 2020;10(6):193. https://doi.org/10.3390/agriculture10060193
  66. Đorđević N, Novaković M, Pejin B, Živković M, Savić A, Mutić J, et al. An insight into chemical composition and biological activity of Montenegrin Vranac red wine. Scientia Horticulturae. 2018;230:142–148. https://doi.org/10.1016/j.scienta.2017.11.033
  67. Mitić MN, Kostic DA, Pavlović AN, Micić RJ, Stojanović BT, Paunović DĐ, et al. Antioxidant activity and polyphenol profile of Vranac red wines from Balkan region. Chemical Industry. 2016;70(3):265–275. https://doi.org/10.2298/HEMIND150130032M
  68. Čomić LR, Radojević ID, Vasić SM, Mladenović KG, Grujović MŽ. Traditionally made red wines produced from an autochthonous grapevine variety as a source of biologically active compounds and their antioxidant potential. Journal of Food and Nutrition Research. 2020;59(4).
  69. Dueñas M, Cueva C, Muñoz-González I, Jiménez-Girón A, Sánchez-Patán F, Santos-Buelga C, et al. Studies on modulation of gut microbiota by wine polyphenols: From isolated cultures to omic approaches. Antioxidants. 2015;4(1):1–21. https://doi.org/10.3390/antiox4010001
  70. Vilela A, Fernanda Cosme F, Inês A. Wine and non-dairy fermented beverages: A novel source of pro- and prebiotics. Fermentation. 2020;6(4)113. https://doi.org/10.3390/fermentation6040113
Как цитировать?
Jovanović-Cvetković T, Savić A , Topalić-Trivunović L , Velemir A , G rbić R . Red wines from the Mostar area: Physicochemical, antioxidative, and antimicrobial properties. Foods and Raw Materials. 2025;13(2):306–319. https://doi.org/10.21603/2308-4057-2025-2-646 
О журнале