ISSN 2308-4057 (Печать),
ISSN 2310-9599 (Онлайн)

Optimizing the utilization of pomelo (Citrus maxima (Brum.) Merr.) seeds as a quality dietary fiber

Аннотация
Orange seeds, often overlooked as waste, have hidden potential since fiber derived from them contains numerous biochemical substances that can enhance the nutritional value of food. We aimed to investigate the impact of pomelo seed fiber on the biscuit dough’s properties (starch and gluten), physicochemical characteristics, and biochemistry, as well as the product’s shelf life. We studied three types of samples: control (no dietary fiber), biscuits with dietary fiber from pomelo (Citrus maxima (Brum.) Merr.) seeds, and biscuits with wheat germ fiber. Scanning electron microscopy was employed to analyze rubbery starch and gluten in the dough, while response surface methods were used to optimize the biscuits’ strength via a central composite design. The product’s shelf life was determined based on microbial contamination levels. ANOVA test and Tukey’s Honestly Significant Difference post hoc test were performed to assess the differences in physicochemical and biochemical properties. Citrus seed fiber influenced rubbery starch and gluten properties, causing significant differences (p < 0.05) in fracturability, total dietary fiber, and Trolox equivalent antioxidant capacity among the three samples. The biscuits enriched with citrus seed fiber contained flavonoid compounds and acylserotonin, with acyl-Nω-methylserotonin dominating in the C22 and C24 homologs. Despite varied evaluations in texture and aroma, the biscuits with citrus seed fiber were well-received for their taste and boasted an extended shelf life (> 12 months). Dietary fiber obtained from C. maxima seeds not only enhanced the nutritional value of the biscuits but also paved the way for innovative healthy food opportunities.
Ключевые слова
Acyl-Nω-methylserotonins, citrus seeds, dietary fiber, N-serotonin, rubbery gluten, rubbery starch, nutritional value
СПИСОК ЛИТЕРАТУРЫ
  1. Rosa An, Era B, Masala C, Nieddu M, Scano P, Fais A, et al. Supercritical CO2 extraction of waste citrus seeds: Chemical composition, nutritional and biological properties of edible fixed oils. European Journal of Lipid Science and Technology. 2019;121:1800502. https://doi.org/10.1002/ejlt.201800502
  2. Rahman MM, Islam F, Parvez A, Azad MAK, Ashraf GM, Ullah MF, et al. Citrus limon L.(lemon) seed extract shows neuro-modulatory activity in an in vivo thiopental-sodium sleep model by reducing the sleep onset and enhancing the sleep duration. Journal of Integrative Neuroscience. 2022;21(1):42. https://doi.org/10.31083/j.jin2101042
  3. Tunjung WAS, Fatonah V, Christy GP, Triono S, Hidayati L, Priyanto D, et al. Effect of growth factor in callus induction and bioactive compounds in seed explant of kaffir lime (Citrus hystrix DC.). Indonesian Journal of Pharmacy. 2020;31(2):61–68. https://doi.org/10.14499/indonesianjpharm31iss2pp61
  4. Rahman MM, Jahan FI, Mim SA. A brief phytochemical investigation and pharmacological uses of citrus seed – A review. PharmacologyOnLine. 2019;1:94–103.
  5. Kulkarni T, Bodhankar S, Sahasrabudhe R. Reversible anti-fertility effects of lemon seeds (Citrus limonum) In female albino rats. National Journal of Basic Medical Sciences. 2012;287.
  6. Kim J, Jayaprakasha GK, Patil BS. Limonoids and their anti-proliferative and anti-aromatase properties in human breast cancer cells. Food and Function. 2013;4:258–265. https://doi.org/10.1039/C2FO30209H
  7. Mahmoud MF, Hamdan DI, Wink M, El-Shazly AM. Hepatoprotective effect of limonin, a natural limonoid from the seed of Citrus aurantium var. bigaradia, on D-galactosamine-induced liver injury in rats. Naunyn-Schmiedeberg's Archives of Pharmacology. 2014;387:251–261. https://doi.org/10.1007/s00210-013-0937-1
  8. Ahsan MT, Maria NN, Tahmida U, Jasmin AA, Chowdhury DUS. Anxiolytic, analgesic and anti-inflammatory effects of Citrus maxima (Burm.) Merr. Seed extract in Swiss albino mice model. Clinical Phytoscience. 2023;9:2. https://doi.org/10.1186/s40816-023-00354-7
  9. Zayed A, Badawy MT, Farag MA. Valorization and extraction optimization of Citrus seeds for food and functional food applications. Food Chemistry. 2021;355:129609. https://doi.org/10.1016/j.foodchem.2021.129609
  10. Akpata MI, Akubor PI. Chemical composition and selected functional properties of sweet orange (Citrus sinensis) seed flour. Plant Foods for Human Nutrition. 1999;54:353–362. https://doi.org/10.1023/A:1008153228280
  11. Yilmaz E, Karaman E. Functional crackers: Incorporation of the dietary fibers extracted from citrus seeds. Journal of Food Science and Technology. 2017;54:3208–3217. https://doi.org/10.1007/s13197-017-2763-9
  12. Karaman E, Yilmaz E, Tuncel NB. Physicochemical, microstructural and functional characterization of dietary fibers extracted from lemon, orange and grapefruit seeds press meals. Bioactive Carbohydrates and Dietary Fibre. 2017;11:9–17. https://doi.org/10.1016/j.bcdf.2017.06.001
  13. Kruk J, Trela-Makowej A, Szymańska R. Acyl-Nω-methylserotonins and branched-chain acylserotonins in lemon and other citrus seeds – New lipids with antioxidant properties and potential pharmacological applications. Biomolecules. 2022;12(10):1528. https://doi.org/10.3390/biom12101528
  14. Blanshard JMV. The glass transition, its nature and significance in food processing. In: Beckett ST, editor. Physico-chemical aspects of food processing. New York: Springer; 1995. p. 17–48. https://doi.org/10.1007/978-1-4613-1227-7_2
  15. Aydeniz Güneşer B, Demirel Zorba NN, Yılmaz E. Antimicrobial activity of cold pressed citrus seeds oils, some citrus flavonoids and phenolic acids. Rivista Italiana Delle Sostanze Grasse. 2018;95:119–131.
  16. Mohammed RMO, Ayoub SMH. Study of phytochemical screening and antimicrobial activity of Citrus aurantifolia seed extracts. American Journal of Analytical Chemistry. 2016;7:254–259. https://doi.org/10.4236/ajac.2016.73022
  17. Cvetnic Z, Vladimir-Knezevic S. Antimicrobial activity of grapefruit seed and pulp ethanolic extract. Acta Pharmaceutica. 2004;54(3):243–250.
  18. Aladekoyi G, Omosulis V, Orungbemi O. Evaluation of antimicrobial activity of oil extracted from three different citrus seeds (Citrus limon, Citrus aurantifolia and Citrus aurantium). International Journal of Scientific Research and Engineering Studies. 2016;3(3):16–20.
  19. Atolani O, Adamu N, Oguntoye OS, Zubair MF, Fabiyi OA, Oyegoke RA, et al. Chemical characterization, antioxidant, cytotoxicity, Anti-Toxoplasma gondii and antimicrobial potentials of the Citrus sinensis seed oil for sustainable cosmeceutical production. Heliyon. 2020;6(2):e03399. https://doi.org/10.1016/j.heliyon.2020.e03399
  20. Trela-Makowej A, Kruk J, Jemioła-Rzemińska M, Szymańska R. Acylserotonins – A new class of plant lipids with antioxidant activity and potential pharmacological applications. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids. 2021;1866(12):159044. https://doi.org/10.1016/j.bbalip.2021.159044
  21. Journal of the American Oil Chemists’ Society. Vol. 77. Arlington: AOAC; 2000.
  22. Budianto B, Suparmi A. Exploration of the biochemical composition of Citrus L. seeds for in-dustrial applications. Grasas y Aceites. 2024;75(2):2102. https://doi.org/10.3989/gya.1204232.2102
  23. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9–10):1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3
  24. Budianto, Feri ZO, Suparmi A, Arifin MJ. Effect of the chemical composition of fluid foods on the rate of fouling processing during sterilization. Vitae. 2023;30(1):349368. https://doi.org/10.17533/udea.vitae.v30n1a349368
  25. Maximum limits of microbial contamination in processed food by fermented vegetable products. Indonesian Drug and Food Control; 2019. 48 p. (In Indonesian).
Как цитировать?
Budianto, Suparmi A, Susanti D. Optimizing the utilization of pomelo (Citrus maxima (Brum.) Merr.) seeds as a quality dietary fiber. Foods and Raw Materials. 2025;13(2):233–241. https://doi.org/10.21603/2308-4057-2025-2-636 
О журнале