ISSN 2308-4057 (Печать),
ISSN 2310-9599 (Онлайн)

Extremophilic bacteria as biofertilizer for agricultural wheat

Wheat (Triticum L.) is a strategically important agricultural crop because its quality and yield provide food security for the population. Biological fertilizers improve the growth and development of agricultural crops. Unlike chemical ones, they have no toxic effect on people and the environment. This research assessed the positive effect of extremophilic microorganisms isolated from coal dump soils of the Kemerovo Region (Russia) on the growth and development of wheat.
The study featured bacterial isolates of Achromobacter denitrificans, Klebsiella oxytoca, and Rhizobium radiobacter, as well as their consortia in four different ratios: 1:1:1 (Consortium A), 2:1:1 (Consortium B), 1:2:1 (Consortium C), 1:1:2 (Consortium D), respectively. The beneficial effect was assessed by determining such factors as nitrogen fixation, solubilization of phosphates, potassium, and zinc, and production of gibberellic acid, siderophores, and hydrogen cyanide. The wheat samples were checked for germination, root length, and stem length.
R. radiobacter demonstrated the best nitrogen fixation properties. Consortium D, with two shares of R. radiobacter, yielded the best results for zinc solubilization. R. radiobacter proved to be the most efficient potassium solubilizer while the isolate of A. denitrificans was the best phosphate solubilizer. The largest amount of gibberellic acid belonged to K. oxytoca. Consortium C, which included two shares of this isolate, appeared to be the most effective siderophore producer. All samples but A. denitrificans were able to produce hydrogen cyanide. The best seed germination rate (84%) belonged to Consortium C, which contained a double share of K. oxytoca. Consortia C and B (two shares of A. denitrificans) had the greatest positive effect on the root length.
Treatment with Consortium B resulted in the longest average stem length. Extremophilic microorganisms isolated from coal dump soils of the Kemerovo Region (Russia) had a good potential as biofertilizers that could improve wheat quality and local food security.
Ключевые слова
Food safety, wheat, biofertilizers, extremophilic microorganisms, seed germination
The research was part of state assignment FZSR-2023-0003: Biopesticides based on extremophilic and endophytic microorganisms as a means to overcome abiotic and biotic stress in agricultural crops in the Kemerovo Region (Kuzbass).
  1. Kumar MS, Reddy GC, Phogat M, Korav S. Role of bio-fertilizers towards sustainable agricultural development: A review. Journal of Pharmacognosy and Phytochemistry. 2018;7(6):1915–1921.
  2. Yadav AN, Kour D, Abdel-Azeem AM, Dikilitas M, Hesham AE-L, Ahluwalia AS. Microbes for agricultural and environmental sustainability. Journal of Applied Biology and Biotechnology. 2022;(S1):1–5.
  3. Karnwal A. Potential of halotolerant PGPRs in growth and yield augmentation of Triticum aestivum var. HD2687 and Zea mays var. PSCL4642 cultivars under saline conditions. BioTechnologia. 2022;103(4):331–342.
  4. Islam MT, Gupta DR, Hossain A, Roy KK, He X, Kabir MR, et al. Wheat blast: A new threat to food security. Phytopathology Research. 2020;2.
  5. Chaves MS, Martinelli JA, Wesp-Guterres C, Graichen FAS, Brammer SP, Scagliusi SM, et al. The importance for food security of maintaining rust resistance in wheat. Food Security. 2013;5:157–176.
  6. Tijjani A, Khairulmazmi A. Global food demand and the roles of microbial communities in sustainable crop protection and food security: An overview. In: Seneviratne G, Zavahir JS, editors. Role of microbial communities for sustainability. Singapore: Springer; 2021. pp. 81–107.
  7. Tudi M, Ruan HD, Wang L, Lyu J, Sadler R, Connell D, et al. Agriculture development, pesticide application and its impact on the environment. International Journal of Environmental Research and Public Health. 2021;18(3).
  8. Fasusi OA, Cruz C, Babalola OO. Agricultural sustainability: Microbial biofertilizers in rhizosphere management. Agriculture. 2021;11(2).
  9. Milentyeva IS, Fotina NV, Zharko MYu, Proskuryakova LA. Microbial treatment and oxidative stress in agricultural plants. Food Processing: Techniques and Technology. 2022;52(4):750–761. (In Russ.).
  10. Salar RK, Purewal SS, Sandhu KS. Bioactive profile, free-radical scavenging potential, DNA damage protection activity, and mycochemicals in Aspergillus awamori (MTCC 548) extracts: A novel report on filamentous fungi. 3 Biotech. 2017;7.
  11. Nosheen S, Ajmal I, Song Y. Microbes as biofertilizers, a potential approach for sustainable crop production. Sustainability. 2021;13(4).
  12. Farzadfar S, Knight JD, Congreves KA. Soil organic nitrogen: an overlooked but potentially significant contribution to crop nutrition. Plant and Soil. 2021;462:7–23.
  13. Gu B, Chen Y, Xie F, Murray JD, Miller AJ. Inorganic nitrogen transport and assimilation in pea (Pisum sativum). Genes. 2022;13(1).
  14. Belashova OV, Kozlova OV, Velichkovich NS, Fokina AD, Yustratov VP, Petrov AN. A phytochemical study of the clover growing in Kuzbass. Foods and Raw Materials. 2024;12(1):194–206.
  15. Soumare A, Diedhiou AG, Thuita M, Hafidi M, Ouhdouch Y, Gopalakrishnan S, et al. Exploiting biological nitrogen fixation: A route towards a sustainable agriculture. Plants. 2020;9(8).
  16. Pal A, Adhikary R, Barman S, Maitra S. Nitrogen transformation and losses in soil: A cost-effective review study for farmer. International Journal of Chemical Studies. 2020;8(3):2623–2626.
  17. Fernandez M, Vernay A, Henneron L, Adamik L, Malagoli P, Balandier P. Plant N economics and the extended phenotype: Integrating the functional traits of plants and associated soil biota into plant – plant interactions. Journal of Ecology. 2022;110(9):2015–2032.
  18. Chen M, Zhu K, Tan P, Liu J, Xie J, Yao X, et al. Ammonia–nitrate mixture dominated by NH4+–N promoted growth, photosynthesis and nutrient accumulation in pecan (Carya illinoinensis). Forests. 2021;12(12).
  19. Chen L, Yang S, Gao J, Chen L, Ning H, Hu Z, et al. Long-term straw return with reducing chemical fertilizers application improves soil nitrogen mineralization in a double rice-cropping system. Agronomy. 2022;12(8).
  20. Rawat P, Das S, Shankhdhar D, Shankhdhar SC. Phosphate-solubilizing microorganisms: Mechanism and their role in phosphate solubilization and uptake. Journal of Soil Science and Plant Nutrition. 2021;21:49–68.
  21. Dey G, Banerjee P, Sharma RK, Maity JP, Etesami H, Shaw AK, et al. Management of phosphorus in salinity-stressed agriculture for sustainable crop production by salt-tolerant phosphate-solubilizing bacteria – A review. Agronomy. 2021;11(8).
  22. Goswami SP, Maurya BR, Dubey AN, Singh NK. Role of phosphorus solubilizing microorganisms and dissolution of insoluble phosphorus in soil. International Journal of Chemical Studies. 2019;7(3):3905–3913.
  23. Etesami H, Emami S, Alikhani HA Potassium solubilizing bacteria (KSB): Mechanisms, promotion of plant growth, and future prospects – A review. Journal of Soil Science and Plant Nutrition. 2017;17(4):897–911.
  24. Sattar A, Naveed M, Ali M, Zahira ZA, Nadeem SM, Yaseen M, et al. Perspectives of potassium solubilizing microbes in sustainable food production system: A review. Applied Soil Ecology. 2019;133:146–159.
  25. Shirale AO, Meena BP, Gurav PP, Srivastava S, Biswas AK, Thakur JK, et al. Prospects and challenges in utilization of indigenous rocks and minerals as source of potassium in farming. Journal of Plant Nutrition. 2019;42(19):2682–2701.
  26. Berger B, Patz S, Ruppel S, Dietel K, Faetke S, Junge H, et al. Successful formulation and application of plant growth-promoting Kosakonia radicincitans in maize cultivation. BioMed Research International. 2018;2018.
  27. Sun F, Ou Q, Wang N, Guo Z, Ou Y, Li N, et al. Isolation and identification of potassium-solubilizing bacteria from Mikania micrantha rhizospheric soil and their effect on M. micrantha plants. Global Ecology and Conservation. 2020;23.
  28. Sarikhani MR, Oustan S, Ebrahimi M, Aliasgharzad N. Isolation and identification of potassium‐releasing bacteria in soil and assessment of their ability to release potassium for plants. European Journal of Soil Science. 2018;69(6):1078–1086.
  29. Setiawati TC, Mutmainnah, L. Solubilization of potassium containing mineral by microorganisms from sugarcane rhizosphere. Agriculture and Agricultural Science Procedia. 2016;9:108–117.
  30. Kamran S, Shahid I, Baig DN, Rizwan M, Malik KA, Mehnaz S. Contribution of zinc solubilizing bacteria in growth promotion and zinc content of wheat. Frontiers in Microbiology. 2017;8.
  31. Saravanan VS, Kumar MR Sa, TM. Microbial zinc solubilization and their role on plants. In: Maheshwari DK, editor. Bacteria in agrobiology: Plant nutrient management. Heidelberg: Springer Berlin; 2011. pp. 47–63.
  32. Georgieff MK. Iron deficiency in pregnancy. American Journal of Obstetrics and Gynecology. 2020;223(4):516–524.
  33. Yiannikourides A, Latunde-Dada GO. A short review of iron metabolism and pathophysiology of iron disorders. Medicines. 2019;6(3).
  34. Jiang H-B, Fu F-X, Rivero-Calle S, Levine NM, Sañudo-Wilhelmy SA, Qu P-P, et al. Ocean warming alleviates iron limitation of marine nitrogen fixation. Nature Climate Change. 2018;8:709–712.
  35. Chen Y, Fan Z, Yang Y, Gu C. Iron metabolism and its contribution to cancer (Review). International Journal of Oncology. 2019;54(4):1143–1154.
  36. Zhang S, Deng Z, Borham A, Ma Y, Wang Y, Hu J, et al. Significance of soil siderophore-producing bacteria in evaluation and elevation of crop yield. Horticulturae. 2023;9(3).
  37. Page MGP. The role of iron and siderophores in infection, and the development of siderophore antibiotics. Clinical Infectious Diseases. 2019;69(7):S529–S537.
  38. Khan A, Singh P, Srivastava A. Synthesis, nature and utility of universal iron chelator – Siderophore: A review. Microbiological Research. 2018;212–213:103–111.
  39. Ravindran P, Kumar PP. Regulation of seed germination: The involvement of multiple forces exerted via gibberellic acid signaling. Molecular Plant. 2019;12(10):1416–1417.
  40. Parwez R, Aftab T, Gill SS, Naeem M. Abscisic acid signaling and crosstalk with phytohormones in regulation of environmental stress responses. Environmental and Experimental Botany. 2022;199.
  41. Mekonnen H, Kibret M. The roles of plant growth promoting rhizobacteria in sustainable vegetable production in Ethiopia. Chemical and Biological Technologies in Agriculture. 2021;8.
  42. Sehrawat A, Sindhu SS, Glick BR. Hydrogen cyanide production by soil bacteria: Biological control of pests and promotion of plant growth in sustainable agriculture. Pedosphere. 2022;32(1):15–38.
  43. Ryabov VA, Vashchenko AYu, Prosekov AYu, Latokhin VA. Disturbed lands of the Kemerovo Region-Kuzbass: genesis and current state. Regional Environmental Issues. 2021;(5):120–123. (In Russ.).
  44. Asyakina LK, Dyshlyuk LS, Prosekov AYu. Reclamation of post-technological landscapes: International experience. Food Processing: Techniques and Technology. 2021;51(4):805–818.
  45. Kvint VL, Alimuradov MK, Zadorozhnaya GV, Astapov KL, Alabina TA, Bakhtizin AR, et al. A conceptual future for the Kuzbass region: Strategic outlines of developmental priorities through 2071, a 50-year perspective. Kemerovo: Kemerovo State University; 2022. 283 p. (In Russ.).
  46. Sharma UC, Datta M, Sharma V. Soil microbes and biofertilizers. In: Sharma UC, Datta M, Sharma V, editors. Soils in the Hindu Kush Himalayas: Management for agricultural land use. Cham: Springer; 2023. pp. 117–144.
  47. Atuchin VV, Asyakina LK, Serazetdinova YuR, Frolova AS, Velichkovich NS, Prosekov AYu. Microorganisms for bioremediation of soils contaminated with heavy metals. Microorganisms. 2023;11(4).
  48. Cordova-Rodriguez A, Rentería-Martínez ME, López-Miranda CA, Guzmán-Ortíz JM, Moreno-Salazar SF. Simple and sensitive spectrophotometric method for estimating the nitrogen-fixing capacity of bacterial cultures. MethodsX. 2022;9.
  49. Belkebla N, Bessai SA, Melo J, Caeiro MF, Cruz C, Nabti E. Restoration of Triticum aestivum growth under salt stress by phosphate-solubilizing bacterium isolated from southern Algeria. Agronomy. 2022;12(9).
  50. Abdenaceur R, Farida B, Mourad D, Rima H, Zahia O, Fatma S-H. Effective biofertilizer Trichoderma spp. isolates with enzymatic activity and metabolites enhancing plant growth. International Microbiology. 2022;25:817–829.
  51. Singh TB, Sahai V, Ali A, Prasad M, Yadav A, Shrivastav P, et al. Screening and evaluation of PGPR strains having multiple PGP traits from hilly terrain. Journal of Applied Biology and Biotechnology. 2020;8(4):38–44.
  52. Ogale S, Yadav KS, Navale S. Screening of endophytic bacteria from the pharmacologically important medicinal plant Gloriosa superba for their multiple plant growth promoting properties. Pharma Innovation. 2018;7(1):208–214.
  53. Tubb RS. Regulation of nitrogen fixation in Rhizobium sp. Applied and Environmental Microbiology. 1976;32(4):483–488.
  54. Naseer I, Ahmad M, Hussain A, Jamil M. Potential of zinc solubilizing Bacillus strains to improve rice growth under axenic conditions. Pakistan Journal of Agricultural Sciences. 2020;57(4):1057–1071.
  55. Nitu R, Rajinder K, Sukhminderjit K. Zinc solubilizing bacteria to augment soil fertility – A comprehensive review. International Journal of Agricultural Sciences and Veterinary Medicine. 2020;8(1):38–44.
  56. Nihala Jabin PN, Ismail S. Solubilization of insoluble potassium by different microbial isolates in vitro condition. International Journal of Current Microbiology and Applied Sciences. 2017;6(10):3600–3607.
  57. Meena VS, Bahadur I, Maurya BR, Kumar A, Meena RK, Meena Sk, et al. Potassium-solubilizing microorganism in evergreen agriculture: An overview. In: Potassium solubilizing microorganisms for sustainable agriculture. New Delhi: Springer; 2016. pp. 1–20.
  58. Blanco-Vargas A, Rodríguez-Gacha LM, Sánchez-Castro N, Garzón-Jaramillo R, Pedroza-Camacho LD, Poutou-Piñales RA, et al. Phosphate-solubilizing Pseudomonas sp., and Serratia sp., co-culture for Allium cepa L. growth promotion. Heliyon. 2020;6(10).
  59. Kaur M, Karnwal A. Screening of plant growth-promoting attributes bearing endogenous bacteria from abiotic stress resisting high altitude plants. Journal of Agriculture and Food Research. 2023;11.
  60. Borah M, Das S, Bora SS, Boro RC, Barooah M. Comparative assessment of multi-trait plant growth-promoting endophytes associated with cultivated and wild Oryza germplasm of Assam, India. Archives of Microbiology. 2021;203:2007–2028.
  61. Li Y, He M, Du Y, Wang X, Zhang H, Dai Z, et al. Indigenous PGPB inoculant from Qinghai-Tibetan Plateau soil confer drought-stress tolerance to local grass Poa annua. International Journal of Environmental Research. 2022;16.
  62. Patel PR, Shaikh SS, Sayyed RZ. Modified chrome azurol S method for detection and estimation of siderophores having affinity for metal ions other than iron. Environmental Sustainability. 2018;1:81–87
  63. Mowafy AM, Khalifa S, Elsayed A. Brevibacillus DesertYSK and Rhizobium MAP7 stimulate the growth and pigmentation of Lactuca sativa L. Journal of Genetic Engineering and Biotechnology. 2023;21.
  64. Walpola BC, Arunakumara KKIU, Yoon M-H. Isolation and characterization of phosphate solubilizing bacteria (Klebsiella oxytoca) with enhanced tolerant to environmental stress. African Journal of Microbiology Research. 2014;8(31):2970–2978.
  65. Basu A, Prasad P, Das SN, Kalam S, Sayyed RZ, Reddy MS, et al. Plant growth promoting rhizobacteria (PGPR) as green bioinoculants: Recent developments, constraints, and prospects. Sustainability. 2021;13(3).
  66. Rao AS, Nair A, More VS, Anantharaju KS, More SS. Extremophiles for sustainable agriculture. In: Singh HB, Vaishnav A, editors. New and future developments in microbial biotechnology and bioengineering. Sustainable agriculture: Advances in microbe-based biostimulants. Elsevier; 2022. pp. 243–264.
Как цитировать?
Faskhutdinova ER, Fotina NV, Neverova OA, Golubtsova YuV, Mudgal G, Asyakina LK, et al. Extremophilic bacteria as biofertilizer for agricultural wheat. Foods and Raw Materials. 2024;12(2):348–360. 
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