ISSN 2308-4057 (Печать),
ISSN 2310-9599 (Онлайн)

A phytochemical study of the clover growing in Kuzbass

In addition to studying bioactive organic compounds in plants, it is increasingly important to determine the biological role of elements in plants growing in environmentally unfavorable areas. One of such regions in Russia is Kuzbass with its intensively developing chemical, metallurgical, and coal mining sectors. In this study, we assessed the plant materials of red clover (Trifolium pratense L.), alsike clover (Trifolium hybridum L.), and white clover (Trifolium repens L.) collected from their natural populations in Kuzbass.
The qualitative and quantitative composition of heavy metals in the clover samples was determined voltammetrically. The contents of molybdenum and phosphorus were measured by the photocolorimetric method. Total nitrogen and protein were determined by the Kjeldahl method. Nickel, cobalt, and chromium were quantified by spectrophotometry.
We analyzed the plant materials of the clover samples for heavy metals and found that the content of lead was the least in red clover and the highest in alsike clover. Copper varied in a larger range and was minimal in red clover compared to that in alsike and white clover. Zinc was found at higher concentrations of in white and red clover compared to that in alsike clover. The levels of cadmium exceeded the maximum permissible concentrations in all the clover samples. We also revealed that the clover samples contained different amounts of various amino acids, including arginine, valine, lysine, glycine, aspartic acid, and alanine.
The plant materials of the clover species growing in Kuzbass can be used to improve the fertility of soil and nitrogen regime. However, the clover species should not be used in bulk feed for farm animals because of high concentrations of cadmium.
Ключевые слова
Clover, Trifolium pratense L., Trifolium hybridum L., Trifolium repens L., soil, microelements, amino acids, heavy metals, plants
This study was part of the Russian Science Foundation (RSF) project “Fundamentals of obtaining bioactive substances from Siberian medicinal plants and creating phytogenic feed additives with immunomodulatory effects on their basis” (Agreement No. 23-16-00113 dated May 15, 2023).
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Как цитировать?
Belashova OV, Kozlova OV, Velichkovich NS, Fokina AD, Yustratov VP, Petrov AN. A phytochemical study of the clover growing in Kuzbass. Foods and Raw Materials. 2024;12(1):194–206. 
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