Abstract

One of promising methods for caseic whey processing is the production of whey protein microparticulate and its consequent application in food technology. This work is aimed at the development of fermented milk product technology using the whey protein microparticulate based on caseic whey. The researches were carried out at the Chair of Animal Product Technology of the Voronezh State University of Engineering Technology, at laboratories of Kombikorm Scientific and Production Complex, Mollab LLC (Voronezh), at the laboratory and pilot shop of the Voronezh Dairy Plant JSC. Standard and generally accepted physical, physico-chemical, chemical, microbiological, physiological and technological research methods were applied by investigators in the course of the study. The sequence of technological operations to obtain the microparticulate covered pre-preparation of curd whey, ultrafiltration, heating to denaturation temperature and dispersion. This is to ensure protein particle formation average sized 3 μm and formation of unique properties of the microparticulate. Valuable properties of the caseic whey microparticulates are applied in the technology of milk product fermentation. The chosen ferment allows preservation of the required fermentation time (5-6 hours) and to obtain the viscous, creamy consistency of the product. The microparticulate is efficiently proportioned, that is, 10%. We studied the option to use the caseic whey microparticulate in the technology of kefir production. It is found that microparticulate facilitated intensification of process fermentation (10 h long) and maturation (6 h), enhancement of kefiran synthesis by kefir fungi microorganisms, intense formation of carbon dioxide and other osmophoric compounds. Formulations of kefir and sour milk drink are suggested that allow using 10% of microparticulate. Production schemes have been developed and customized to the HACCP system that consider introduction of supplementary operations to obtain the caseic whey microparticulate. The technological solutions developed are known for the following key advantages: realization of the complete production cycle; increase in food biological value; reduction of technological process length due to souring stimulation

Keywords

Caseic whey, whey protein microparticulate, fermented milk products

REFERENCES

1. Gavrilov G.B. and Kravchenko E.F. Ways of the efficient application of whey. Dairy industry, 2012, no. 7, pp. 47-49. (In Russian).
2. Evdokimov I.A. Current methods of milk whey membrane processing at centralized entity. Milk Processing, 2012, no. 4, pp. 34-36. (In Russian).
3. Khramtsov A.G. Novatsii molochnoy syvorotki [Milk whey innovations]. St. Petersburg: Professija Publ., 2016. 490 p.
4. Khramtsov A.G. Fenomen molochnoy syvorotki [Milk whey phenomenon]. St. Petersburg: Professija Publ., 2011. 900 p.
5. Damodaran S. and Paraf A. (eds) Food Proteins and Their Applications. Boca Raton: CRC Press LLC, 2007. 694 r.
6. Hurley W.L. Milk protein. Vol. 1. Rijeka, Croatia: InTech, 2012. 352 p.
7. Evdokimov I.A., Volodin D.N., Mikhneva V.A., et al. Curds and curds products with milk whey and its components. Dairy industry, 2011, no. 11, pp. 62-63. (In Russian).
8. Smirnova I.A., Lobacheva E.M., and Gulbani A.J. Applying of microparticiulated whey proteins in milk products. Dairy industry, 2014, no. 6, pp. 28-30. (In Russian).
9. Smirnova I.A., Romanovskaya I.V., and Shtrigul V.K. Method of obtaining microparticulated casein and the possibility of its application in the production of nonfat fermented milk products. Foods and Raw Materials, 2013, vol. 1, no. 1, pp. 26-32. DOI: 10.12737/1514.
10. Melnikova E.I. and Stanislavskaya E.B. Mikropartikulyaty syvorotochnykh belkov kak imitatory molochnogo zhira v proizvodstve produktov pitaniya [Whey protein microparticulates as milk fat imitators in food production]. Fundamental research, 2009, no. 7 (application), pp. 23-23. (In Russian). Available at: http://www.fundamentalresearch.ru/en/article/view?id=2108 (accessed 7 October 2016).
11. Singer N.S. and Dunn J.M. Protein microparticulation: The principle and the process. The Journal of the American College of Nutrition, 1990, vol. 9, no. 4, rr. 388-397. DOI: 10.1080/07315724.1990.10720397.
12. Hayakawa J., Yamada Y., and Fujio Y. Microparticulation by jet mill grinding of protein powders and effects of hydrophobicity. Journal of Food Science, 1993, vol. 58, no. 5, rr. 1026-1029. DOI: 10.1111/j.1365-2621.1993.tb06104.x.
13. Cheftel J.C. and Dumay E. Microcoagulation of proteins for development of “creaminess”. Food Reviews International, 1993, vol. 9, no. 4, pp. 473-502. DOI: 10.1080/87559129309540975.
14. Roller S. and Jones S.A. (eds) Handbook of Fat Replacers. Boca Raton: CRC Press LLC, 1996, 295 p.
15. Dymar O.V. Technological aspects of applying microparticulates of whey proteins at milk products manufacturing. Dairy industry, 2014, no. 6, pp. 18-21. (In Russian).
16. Melnikova E.I., Stanislavskaya E.B., and Korotkov E.G. Preparation and use of whey proteinmicroparticulate in synbiotic drink technology. Foods and Raw Materials, 2015, vol. 3, no. 2, pp. 96-104. DOI: 10.12737/13125.
17. Cheirsilp B., Shimizu H., and Shioya S. Modelling and optimization of environmental conditions for kefiran production by Lactobacillus kefiranofaciens. Applied Microbiology and Biotechnology, 2001, vol. 57, no. 5-6, pp. 639-646. DOI: 10.1007/s00253-001-0846-y.
18. Dubois M., Gilles K.A., Hamilton J.K., et al. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 1956, vol. 28, no. 3, pp. 250-356. DOI: 10.1021/ac60111a017.
19. Enikeev R.R., Boboshko D.N., Rudenko E.Yu., and Zimichev A.V. Level of the polysaccharide produced by lactic acid bacteria in kefir. Dairy industry, 2010, no. 7, pp. 64-65. (In Russian).
20. Sandoval-Castilla O., Lobato-Calleros C., Aguirre-Mandujano E., and Vernon-Carter E.J. Microstructure and texture of yogurt as influenced by fat replacers. International Dairy Journal, 2004, vol. 14, no. 2, pp. 151-159. DOI:10.1016/S0958-6946(03)00166-3.
21. Yazici F. and Akgun A. Effect of some protein based fat replacers on physical, chemical, textural, and sensory properties of strained yoghurt. Journal of Food Engineering, 2004, vol. 62, no. 3, pp. 245-254. DOI: 10.1016/S0260-8774(03)00237-1.
22. Lobato-Calleros C., Martínez-Torrijos O., Sandoval-Castilla O., et al. Flow and creep compliance properties of reduced-fat yoghurts containing protein-based fat replacers. International Dairy Journal, 2004, vol. 14, no. 9, pp. 777-782. DOI: 10.1016/j.idairyj.2004.02.012.
23. Torres I.C., Janhøj T., Mikkelsen B.T., and Ipsen R. Effect of microparticulated whey protein with varying content of denatured protein on the rheological and sensory characteristics of low-fat yoghurt. International Dairy Journal, 2011, vol. 21, no. 9, pp. 645-655. DOI: 10.1016/j.idairyj.2010.12.013.
24. Dissanayake M., Liyanaarachchi S., and Vasiljevic T. Functional properties of whey proteins microparticulated at low pH. Journal of Dairy Science, 2012, vol. 95, no. 4, pp. 1667-1679. DOI: 10.3168/jds.2011-4823.
25. Liu K., Tian Y., Stieger M., et al. Evidence for ball-bearing mechanism of microparticulated whey protein as fat replacer in liquid and semi-solid multi-component model foods. Food Hydrocolloids, 2016, vol. 52, pp. 403-414. DOI: 10.1016/j.foodhyd.2015.07.016.
26. Korzhov R.P., Ponomarev A.N., Melnikova E.I., and Bogdanova E.V. Selection of starter cultures for kefir product with reduced allergenicity. Dairy industry, 2015, no. 4, pp. 30-31. (In Russian).
27. Kwon O.K., Ahn K.S., Lee M.Y., et al. Inhibitory effect of kefiran on ovalbumin-induced lung inflammation in a murine model of asthma. Archives of Pharmacal Research, 2008, vol. 31, no. 12, pp. 1590-1596. DOI: 10.1007/s12272-001-2156-4.