Abstract

The fractional composition of proteins of the cod-fish family - polar cod ( Boreogadus saida ) and blue whiting ( Micromesistius poutassou ) which are currently underutilized in the food industry has been studied. A method of multiple extraction of homogenized fish raw material with solutions of increasing ionic strength and pH was used for protein fractionation. Water-soluble (WSP), salt-soluble (SSP) and alkaline-soluble (ASP) fractions of fish proteins have been separated. The content of protein in a whole fish and its aqueous extracts was determined by the Kjeldahl method as well as photometric method. The amino acid composition of the proteins was studied by high-performance liquid chromatography. Protein of blue whiting comprises 92% of WSP and SSP fractions; while polar cod comprisesonly 60%. All proteins under study contain the full set of the amino acids including essential ones. The content of lysine both in non-fractionated proteins contained in a whole fish and their fractions is considerable (from 59.5 to 121 g kg-1). When evaluating the biological value of proteins, their usefulness is established (there are no limiting amino acids). The data of the rationality coefficient Rc which presents the balance of the amino acid composition of the proteins indicate the high biological value of proteins of the whole polar cod and their ASP fraction. The fish species being studied can be used for production high-grade protein products - fish hydrolysates and isolates. The possibility of using unrefined fish is shown that is peculiarity of novel food technologies for obtaining protein products of high biological value

Keywords

Amino acid balance, blue whiting, fractional and amino acid composition, polar cod, protein biological value

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