ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

TECHNOLOGICAL PROPERTIES OF MILK OF COWS WITH DIFFERENT GENOTYPES OF KAPPA-CASEIN AND BETA-LACTOGLOBULIN

Abstract
The presence of the desirable alleles and genotypes of casein and whey protein genes in the genome of cows affects the milk protein content, quality and technological properties of their milk. Two important properties of milk its producibility is judged on are cheeseability and heat resistance. The present studies aimed at estimating the technological properties of milk of black-motley × Holstein and Kholmogorskaya breeds cows of the Tatarstan type with different kappa-casein ( CSN3 ) and beta-lactoglobulin ( BLG ) genotypes. The study was carried out using a sampling of the first-calf cows of 5 cattle-breeding farms of the Republic of Tatarstan. In animals, the CSN3 and BLG genotypes have been determined by a PCR-RFLP analysis. The cheeseability, heat resistance and thermostability of milk have been estimated using standard methods. The studies have established that the CSN3 and BLG genotypes of cows affected the condition of a casein clot and duration of milk clotting time. The best cheese-making properties of milk were inherent in the animals with the BB and AB genotypes of the CSN3 and BLG genes. They were superior to the coevals with the AA genotype in terms of the highest yield of the desired dense casein clot and the shortest duration of milk clotting time. The first-calf cows, which are the carriers of an A allele of the CSN3 gene, were superior to the animals with the BB genotype of the CSN3 gene on the thermostability of milk including that on the proportion of animals with this milk characteristic. The BLG genotype of the studied animals did not significantly affect the thermostability of milk. Moreover, the highest thermostability of milk was characteristic of black-motley × Holstein cows with the AA genotype.
Keywords
Cow, milk, cheeseability, thermostability, allele, genotype, CSN3, BLG, PCR, RFLP
REFERENCES
  1. Gorbunova Yu.A. and Overchenko A.S. Milk applicability to cheese making and methods of its increase. Agrarian Education and Science, 2014, no. 3, pp. 1-5. (In Russian).
  2. Kharisov M.M. Belkovyy sostav, tekhnologicheskie svoystva i kachestvo molochnoy produktsii u chistoporodnogo skota i pomesey bestuzhevskaya kh ayrshirskaya raznogo genotipa [Protein composition, technological properties and quality of dairy products in purebred cattle and different genotypes of Bestuzhev x Ayrshire hybrids]. Diss. Cand. Sci. (Biol.). Kazan, 2003. 136 p.
  3. Mukhametgaliev N.N. Ispol'zovanie geneticheskoy i paratipicheskoy izmenchivosti belkovogo sostava moloka dlya uluchsheniya tekhnologicheskikh svoystv syr'ya i povysheniya kachestva molochnykh produktov [Using the genetic and paratypic variability of protein composition of milk to improve the technological properties of raw milk and improve the quality of dairy products]. Diss. Dr. Sci. (Biol.). Kazan, 2006. 344 p.
  4. Khromova G.L., Baylova N.V., Pilyugina E.A., and Volokitina I.V. Heat stability of milk from the main cow breeds in the central Chernozem region in the context of modern production techniques. Vestnik of the Voronezh State Agrarian University, 2013, no. 1, pp. 251-257. (In Russian).
  5. Petrov A.N., Galstyan A.G., Radaeva I.A., et al. Indicators of quality of canned milk: Russian and international priorities. Foods and Raw Materials, 2017, vol. 5, no. 2, pp. 151-161. DOI: 10.21603/2308-4057-2017-2-151-161.
  6. Strizhko M., Kuznetsova A., Galstyan A., et al. Development of osmotically active compositions for milk-based products with intermediate humidity. Bulletin of the International Dairy Federation, 2014, no. 472, pp. 35-40.
  7. Rjabova A.E., Kirsanov V.V., Strizhko M.N., et al. Lactose crystallization: Current issues and promising engineering solutions. Foods and Raw Materials, 2013, vol. 1, no. 1, pp. 66-73. DOI: 10.12737/1559.
  8. Galstyan A.G., Petrov A.N., and Semipyatniy V.K. Theoretical backgrounds for enhancement of dry milk dissolution process: mathematical modeling of the system “solid particles-liquid”. Foods and Raw Materials, 2016, vol. 4, no. 1, pp. 102-109. DOI: 10.21179/2308-4057-2016-1-102-109.
  9. Petrov A.N., Khanferyan R.A., and Galstyan A.G. Current aspects of counteraction of foodstuff's falsification. Voprosy Pitaniya, 2016, vol. 85, no. 5, pp. 86-92.
  10. Prosekov A.Yu. Theory and practice of prion protein analysis in food products. Foods and Raw Materials, 2014, vol. 2, no. 2, pp. 106-120. DOI: 10.12737/5467.
  11. Piskaeva A.I., Sidorin Yu.Yu., Dysluk L.S., Zhumaev Yu.V., and Prosekov A.Yu. Research on the influence of silver clusters on decomposer microorganisms and E. Coli bacteria. Foods and Raw Materials, 2014, vol. 2, no. 1, pp. 62-66. DOI: 10.12737/4136.
  12. Velmatov A.P., Neyaskin N.N., and Tel'nov N.O. The effects of kappa-casein and beta-lactoglobulin genotypes on milk productivity and technological properties of milk of red-and-white cows in Mordovia Republic. Ogarev-Online, 2017, vol. 90, no. 1, p. 9. (In Russian).
  13. Bijl E., van Valenberg H., Sikkes S., et al. Chymosin-induced hydrolysis of caseins: Influence of degree of phosphorylation of alpha-s1-casein and genetic variants of beta-casein. International Dairy journal, 2014, vol. 39, pp. 215-221. DOI: 10.1016/j.idairyj.2014.07.005.
  14. Tyulkin S.V., Zagidullin L.R., Shaydullin S.F., et al. Polymorphism of beta casein gene for herds of cattle in the Republic of Tatarstan. Scientific notes of the Kazan State Academy of Veterinary Medicine, 2016, vol. 228, no. 4, pp. 78-81. (In Russian).
  15. Valiullina E.F., Zaripov O.G., Tyulkin S.V., Akhmetov T.M., and Vafin R.R. Characteristics of stud bulls with different combinations of kappa-casein & beta-lactoglobulin genotypes for the milk productivity of their mothers. Veterinary practice, 2007, no. 4, pp. 59-63. (In Russian).
  16. Goncharenko G.M., Goryacheva T.S., Rudishina N.M., Medvedeva N.S., and Akulich E.G. Comparative evaluation of cheese availability of milk of Simmental and Red Steppe breeds taking into account the genotypes of k-casein gene. Bulletin of the Altai State Agricultural University, 2013, vol. 110, no. 12 , pp. 113-117. (In Russian).
  17. Loretts O.G. Dairy efficiency and technological properties of milk with different genotypes for kappa-casein. Veterinariya Kubani, 2014, no. 2, pp. 6-8. (In Russian).
  18. Gustavsson F., Buitenhuis A.J., Johansson M., et al. Effects of breed and casein genetic variants on protein profile in milk from Swedish Red, Danish Holstein, and Danish Jersey cows. Journal of Dairy Science, 2014, vol. 97, no. 6, pp. 3866-3877. DOI: 10.3168/jds.2013-7312.
  19. Valitov F., Rakina Yu., Gareeva I., and Dolmatova I. The influence of cattle β-lactoglobulin gene polymorphism to the milk quality. Dairy and beef cattle breeding, 2011, no. 6, pp. 15-17. (In Russian).
  20. Tjulkin S.V., Akhmetov T.M., and Muratova A.V. The Characteristics of stud bulls with different genotypes of alpha-lactalbumin by origin. Scientific notes of the Kazan State Academy of Veterinary Medicine, 2013, vol. 216, pp. 324-328. (In Russian).
  21. Abeykoon C.D., Rathnayake R.M.C., Johansson M., et al. Milk coagulation properties and milk protein genetic variants of three cattle breeds/types in Sri Lanka. Procedia Food Science, 2016, vol. 6, pp. 348-351. DOI: 10.1016/j.profoo.2016.02.070.
  22. Valitov F.R. and Dolmatova I.Yu. Vliyanie polimorfizma molochnykh belkov na kachestvo i tekhnologicheskie svoystva moloka [Influence of polymorphism of milk proteins on the quality and technological properties of milk]. Materialy 10-oy Vserossiyskoy konferentsii-shkoly molodykh uchënykh s mezhdunarodnym uchastiyem «Sovremennyye dostizheniya i problemy biotekhnologii sel’skokhozyaystvennykh zhivotnykh, BioTekhZH - 2015» [Proc. of the 10th All-Russian Conference-School of Young Scientists with International Participation "Modern Achievements and Problems of Biotechnology of Agricultural Animals, BioTechZh-2015"]. Dubrovitsy, 2015, pp. 50-56.
  23. Barroso A., Dunner S., and Canon J. Detection of bovine kappa-casein variants A, B, C and E by means of polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP). Journal of Animal Science, 1998, vol. 76, no. 6, pp. 1535-1538.
  24. Vafin R.R., Akhmetov T.M., Valiulllina E.F., Zaripov O.G., and Tyulkin S.V. An optimization of cattle genotyping means by kappa-casein gene. Veterinary practice, 2007, no. 2, pp. 54-69. (In Russian).
  25. Medrano J.F. and Aguilar-Cordova E. Polymerase chain reaction amplification of bovine β-lactoglobuline genomic sequences and identification of genetic variants by RFLP analysis. Animal Biotechnology, 1990, no. 1, pp. 73-77.
  26. Merkur'eva E.K. Biometriya v selektsii i genetike sel'skokhozyaystvennykh zhivotnykh [Biometrics in the selection and genetics of farm animals], Moscow: Kolos Publ., 2008. 360 p.
  27. Tamarova R., Yarlykov N., and Mordvinova V. Complex estimation of cheese suitability milk of cows of the yaroslavl breed. Dairy and beef cattle breeding, 2011, no. 3, pp. 25-26. (In Russian).
  28. Meshcherov Sh.R., Meshcherov R.K., and Kalashnikova L.A. Quality of milk from Holmogor cows with different genotypes of kapp-casein. Cheesemaking and Buttermaking, 2009, no. 4, pp. 54-55. (In Russian).
  29. Soboleva N.V., Efremov A.A., and Karamaev S.V. Quality of cheese made from milk of cows with different kappacasein genotypes. Proceedings of the Orenburg State Agrarian University, 2011, vol. 3, no. 31-1, pp. 180-182. (In Russian).
  30. Volokhov I.M., Pashchenko O.V., and Skachkov D.A. Quality of milk and dairy products of animals created by the Volga type of red-and-white cattle of different genotypes for kappa-casein. News of the Nizhnevolzhsk Agro- University Complex: Science and Higher Professional Education, 2012, no. 3, pp. 160-164. (In Russian).
  31. Telnov N.O. Influence of kappa-casein genotype on milk productivity and technological properties of milk of cows of red-motley breed in the republic of Mordovia. Vestnik of Ulyanovsk state agricultural academy, 2016, vol. 3, no. 2, pp. 160-163. (In Russian).
  32. Perna A., Intaglietta I., Gambacorta E., and Simonetti A. The influence of casein haplotype on quality, coagulation, and yield traits of milk from Italian Holstein cows. Journal of Dairy Science, 2016, vol. 99, no. 5, pp. 3288-3294. DOI: 10.3168/jds.2015-10463.
  33. Jensen H.B., Poulsen N.A., Andersen K.K., et al. Distinct composition of bovine milk from Jersey and Holstein- Friesian cows with good, poor, or noncoagulation properties as reflected in protein genetic variants and isoforms. Journal of Dairy Science, 2012, vol. 95, no. 12. pp. 6905-6917. DOI: 10.3168/jds.2012-5675.
  34. Soloshenko V.A., Popovski Z.T., Goncharenko G.M., et al. Association of polymorphism of κ-casein Gene and Its Relationship with Productivity and Qualities of a Cheese Production. Research Journal of Pharmaceutical, Biological and Chemical Sciences, 2016, vol. 7, no. 4, pp. 3214-3221.
  35. Gregorio P.Di., Grigoli A.Di., Trana A.Di., et al. Effects of different genotypes at the CSN3 and LGB loci on milk and cheese-making characteristics of the bovine Cinisara breed. International Dairy journal, 2017, vol. 17, pp. 1-5. DOI: 10.1016/j.idairyj.2016.11.001.
  36. Kübarsepp I., Henno M., Viinalass H., and Sabre D. Effect of κ-casein and β-lactoglobulin genotypes on the milk rennet coagulation properties. Agronomy Research, vol. 3, no 1, pp. 55-64.
  37. Tanaskovska B.R., Srbinovska S., Andonov S., et al. Genotipization of k-Casein in Holstein-Friesian sattle in Macedonia and its association with some milk properties. International Journal of Agriculture Innovations and Research, 2016, vol. 5, no. 2, pp. 266-270.
  38. Ketto I.A., Knutsen T.M., Oyaas J., et al. Effects of milk protein polymorphism and composition, casein micelle size and salt distribution on the milk coagulation properties in Norwegian Red cattle. International Dairy journal, 2017, vol. 70. pp. 55-64. DOI: 10.1016/j.idairyj.2016.10.010.
  39. Dyman T.M. and Plivachuk O.P. Effect of beta-lactoglobulin genotypes on composition and technological properties of milk in Ukrainian black-and-white dairy cattle. Animal breeding and genetics, 2015, no. 49, pp. 187-192.
  40. Hallen E., Allmere T., Lunden A., and Andren A. Effect of genetic polymorphism of milk proteins on rheology of acid-induced milk gels. International Dairy Journal, 2009, vol. 19, nos 6-7, pp. 399-404. DOI: 10.1016/j.idairyj.2008.08.005.
  41. Rahali V. and Menard J.L. Influence des variants genetiques de la -lactodlobuline et la -caseine sur la composition du lait et son aptitude fromagere. Lait, 1991, vol. 71, pp. 275-297. DOI: 10.1051/lait:1991321.
How to quote?
About journal

Download
Contents
Abstract
Keywords
References