Abstract

In the work, activated cultures of propionic acid bacteria were found to exhibit high antimutagenic activity and adhesion properties, synthesize considerable amount of corrinoids and heme-containing enzymes. Increase of iron concentration in the medium was shown to intensify synthesis of extracellular metabolites promoting adaptation of the culture to the metal. Optimal technological parameters for isolation of casein phosphopeptides were determined. Ability of phosphopeptides to efficiently solubilize divalent iron was confirmed. Relationship between iron concentration and extent of solubilization was established. Iron chelated with casein phosphopeptides was noted to stay in divalent form for prolonged period.

Keywords

propionic acid bacteria, catalase, peroxidase, superoxide dismutase, casein phosphopeptides, iron solubilization

REFERENCES

1. Avtsyn, A., Zhavoronkov, A., Rosh, M., and Strochlova. L., Mikroelementozy cheloveka (Human Microelementoses), Moscow, 2005.
2. Vorob´eva, L., Propionovokislye bacterii (Propionic Acid Bacteria), Moscow, 1999.
3. Gapparov, M., and Stan, E., Vliyanie cazeinovykh fosfopeptidov na biodostupnost´ mineralov (Effect of casein phosphopeptides on bioavailability of minerals), Voprosy pitaniya (Nutrition Aspects), 2003, vol. 6, pp. 40–44.
4. Savijoki, K., and Palva, A., Purification and molecular characterization of tripeptidas from Lactobacillus helveticus, Appl. Environ. Microbiol., 2000, vol. 66, pp. 794–800.
5. Furlund, C.B., Ulleberg, E.K., Devold, T.G., Flengsrud, R., and Jecobsen, M., Identification of lactoferrin peptides generated by digestion with human gastrointestinal enzymes, J. Dairy Science, 2013, vol. 96, pp. 75–88.
6. Khamagaeva, I., Kachanina, L., and Tumurova, S., Biotekhnologiya zakvasok propionovokislykh bakterii (Biotechnology of Propionic Acid Bacteria Starters), Ulan-Ude, 2006.
7. Smirnov, I., Kol´tsov, G., Gladun, V., and Levina, A., Referentnyi metod opredeleniya zheleza (Reference Method for Iron Determination), Problemy gematologii i perelivaniya krovi (Hematology and Blood Transfusion Problems), 1999, vol. 1, pp. 50–53.
8. Korolyuk, M.A., Ivanova, L.I., Maiorova, N.O., and Tokarev, V.E., Metod opredeleniya aktivnosti katalazt (Method for determination of catalase activity), Laboratornoe delo (Laboratory Science), 1988, vol. 1, p. 16.
9. Lebedeva, O.V., Ugarova, N.N., and Berezin, I.V., Kineticheskoe izuchenie reaktsii okisleniya o-dianizidina perekis´yu vodoroda v prisutstvii peroksidazy khrena (Kinetic Study of o-dianizidin Oxydation with Hydrogen Peroxide in the Presence of Horseradish Peroxidase), Biokhimiya (Biochemistry), 1977, vol. 42, no. 8, pp. 1372–1379.
10. Sirota, T.V., Novyi podkhod v issledovanii protsessa autookisleniya adrenalina i ispol´zovanie ego dlya izmereniya aktivnosti superoksiddismutazy (New Approach to Investigation of Adrenalin Autooxidation and its Application to Measurement of Superoxide Dismutase Activity), Voprosy meditsynskoi khimii (Medicinal Chemistry Issues), 1999, vol. 3, pp. 22–33.
11. Brilis, V.I., Brilene, T.A., Lentsner, Kh.P., and Lentsner, A.A., Mikrobiologiya (Microbiology), 1982, vol. 9, pp. 75–78.
12. Nevo, A.S., Pshenichnikova, A.B., Skladnev, D.A., and Shvets, V.I., Vliyanie deiterometanola i oksida deiteriya na rostovye kharakteristiki i biosintez ekzopolisakharida obligatnymi metilotrofnymi bakteriayami (Effect of deuteromethanol and deuterium oxide on growth characteristics and biosynthesis of exopolysaccharide by obligate methylotrophic bacteria), Biotekhnologiya (Biotechnology), 2003, vol. 6, pp. 38–46.
13. Kanopkaite, S., Kobalaminy (Cobalamines), Vilnius: Mokslas, 1978.
14. Tutel´yan, V.A., Spirichev, V.B., Shatnyuk, and L.N., Korrektsyya mikronutrientnogo defitsyta — vazhneishii aspekt kontseptsii zdorovogo pitaniya naseleniya Rossii (Correction of micronutrient defficiency as a key aspect of the concept of healthy nutrition in Russia), Voprosy pitaniya (Nutrition Issues), 1999, vol. 1, pp. 3–11.
15. Khamagaeva, I.S., Krivonosova, A.V., and Radnaeva, R.B., Biotekhologicheskii potentsyal propionovokislykh bacterii (Propionic acid bacteria potential for biotechnology), Molochnaya promyshlennost´ (Dairy Industry), 2007, vol. 11, pp. 30–31.