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Modern methods of slime waste disposal utilized by wastewater treatment plants were analyzed. Domestic and foreign experience in the application of silver clusters in reducing pathogenic and conditionally pathogenic microorganisms inhabiting the waste sludge was studied. The main mechanisms of bactericidal and bacteriostatic effects silver clusters are capable of exerting on microorganisms were considered. Strains of microorganisms with the ability to recycle organic and inorganic substances present in the waste sludge into ecologically pure humus fertilizer in the course of their vital activity were selected. The effects of different concentrations of silver clusters on growth and development of the microorganisms decomposing organic compounds (Microbacterium terregens BSB-570, Streptococcus termophilus St5, Lactobacillus sp. 501 (2A4), Rhodococcus erythropolis, Bacillus fastidiosus, Arthrobacter sp. (Arthrobacter paraffineus) ATCC 15591, etc.), as well as on the Escherichia coli bacteria chosen as a model organism, were studied. For the first time, decomposers modified with silver clusters, i.e. resistant to high concentrations of silver clusters, able not only to grow, but also to reproduce normally and, consequently, to recycle the waste sludge, were obtained. Bacteriostatic and bactericidal concentrations of silver clusters with respect to decomposers and optimal concentration, at which the useful microorganisms are able to grow and reproduce actively and the pathogenic Escherichia coli die, were determined.
- Arustamov, E.A., Bezopasnost’ zhiznedeyatel’nosti (Safety of Living), Moscow: Dashkov and co., 2006.
- Feng, P., Weagent, S.D., Grant, M.A., and Burkhardt, W., Enumeration of Escherichia coli and the Coliform Bacteria, in Bacteriological Analytical Manual, Gaithersburg, MD: AOAC Intl, 2002, 8th edition.
- Kostyleva, R.N., and Burmistrov, V.A., Comparative analysis of bactericidal activity of colloidal silver preparations, in Serebro i vismut v meditsyne (Silver and Bismuth in Medicine), Novosibirsk, 2005, pp. 53–60.
- Trevors, J.T., Silver Resistance and Accumulation in Bacteria, Enzyme Microb. Technol., 1987, no. 9, pp. 331–333.
- Wells, T.N., Scully, P., Paravicini, G., Proudfoot, A.E., and Payton, M.A., Mechanism of Irreversible Inactivation of Phosphomannose Isomerases by Silver Ions and Flamazine, Biochemistry, 1995, no. 34 (24), pp. 7896–7903.
- Ivanov, V.N., Larionov, G.M., and Kulish, N.I., Some experimental and clinical data on application of silver ions in management of drug-resistant microorganisms, in Serebro v meditsyne i tekhnike (Silver in Medicine and Technology), Novosibirsk: Siberian Branch of the Russian Academy of Medical Sciences, 2005, pp. 53–62.
- Savadyan, E.Sh., Mel’nikova, V.M., and Belikov, G.P., Modern trends in application of silver-based antiseptics, Antibiotiki i khimioterpiya (Antibiotics and Chemotherapy), 1999, vol. 34, no. 11, pp. 874–878.
- Tolgskaya, M.S., and Chumakov, A.A., Bol’shaya meditsynskaya entsyklopediya (Great Medical Encyclopedia), Petrovskii, B.V., Ed., Moscow: Sovetskaya Encicklopediya, 1984, vol. 2, pp. 142–143.
- Schreurs, W.J., and Rosenberg, H., Effect of Silver Ions on Transport and Retention of Phosphate by Escherichia coli, J. Bacteriol., 2002, vol. 152, no. 1, pp. 7–13.
- Dibrov, P., Dzioba, J., Gosink, K.K., and Häse, C.C., Chemiosmotic Mechanism of Antimicrobial Activity of Ag+ in Vibrio cholera, Antimicrob. Agents Chemother., 2002, vol. 46, no. 8, pp. 2668–2670.
- Mullen, M.D., Wolf, D.C., Ferris, F.G., Beveridge, T.J., Flemming, C.A., and Bailey, G.W., Bacterial Sorption of Heavy Metals, Appl. Environ. Microbiol., 1989, vol. 55, no. 12, pp. 3143–3149.
- Müller, H.E., Oligodynamic Action of 17 Different Metals on Bacillus subtilis, Enterobacteriaceae, Legionellaceae, Micrococcaceae, and Pseudomonas aeruginosa, Zentralbl. Bakteriol. Mikrobiol. Hyg. B, 1985, vol. 182, no. 1, pp. 95–101.
- Kul’skii, P.A., Serebryanaya voda (Silver Water), Kiev: Naukova Dumka, 1987.