Antioxidant and anti-diabetic activity of pomegranate (Punica granatum L.) leaves extracts

Mohammed Cheurfa ^a,b

Mohamed Achouche ^a

Ahmed Azouzi ^a

Abdalbasit A. Mariod ^c,d

Affiliation

a University of Djillali Bounaama Khemis Miliana, Khemis Miliana, Algeria

b Hassiba Benbouali University of Chlef, Chlef, Algeria

c University of Jeddah, Alkamil, Saudi Arabia

d Ghibaish College of Science and Technology, Ghibaish, Sudan

Copyright ©Cheurfa et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.

Received 13 April, 2020 | Accepted in revised form 05 May, 2020 | Published 25 August, 2020

DOI: http://doi.org/10.21603/2308-4057-2020-2-329-336

Abstract

Introduction. This study aimed to evaluate the antioxidant and anti-diabetic activity of aqueous and hydroalcoholic extracts of pomegranate (Punica granatum L.) leaves in vitro, as well as to determine the content of polyphenols, flavonoids, and flavonols.
Study objects and methods. The antioxidant activity was determined by the DPPH test using the free radical 1,1-diphenyl-2-picrylhydrazyle and the FRAP method, as well as by measuring total antioxidant capacity and the hydrogen peroxide scavenging activity.
Results and discussion. The content of total polyphenols varied between 4.43 ± 0.3 and 12.66 ± 1.6 mg EAG/g. The highest content of flavonoids was observed in the hydroalcoholic extract of P. granatum leaves (P < 0.05). The flavonol contents in the hydroalcoholic and aqueous extracts were 7.68 ± 0.6 and 9.20 ± 2.8 mg EQ/g, respectively. The IC₅₀ of the antioxidant potential of the hydroalcoholic and aqueous extracts was 32.4 ± 1.109 and 35.12 ± 4.107 mg/mL, respectively. According to the DPPH test, the aqueous extract was the least active (IC₅₀ = 14.15 ± 1.513 mg/mL). The highest percentage of hydrogen peroxide trapping was found in the aqueous extract (45.97 ± 6.608 %). The inhibition of α-amylase showed an IC₅₀ of between 9.804 ± 0.67 and 19.011 ± 9.82 mg/mL in the aqueous and hydroalcoholic extracts, respectively. The inhibition of glucose uptake by yeast recorded a high inhibitory capacity at 50 mg/mL of glucose.
Conclusion. We found that the antioxidant and anti-diabetic activity of P. granatum leaves extracts was due to the presence of bioactive compounds such as flavonoids, which is why they are effective in preventing diabetes and its complications.

Keywords

Punica granatum L., plant extracts, antioxidant activity, anti-diabetic activity, flavonoids

REFERENCES

Sreedevi P, Vijayalakshmi K, Venkateswari R. Phytochemical evaluation of Punica granatum L. leaf extract. International Journal of Current Pharmaceutical Research. 2017;9(4):14–18. DOI: https://doi.org/10.22159/ijcpr.2017v9i4.1159.
Amjad L, Shafighi M. Antioxidant activity of leaf different extracts in Punica granatum. International Journal of Biological and Medical Research. 2012;3(3):2065–2067.
Vinodhini S, Shri Preethi M, Nusrath Fathima N, Kushwaha SS, Devi Rajeswari V. Antioxidant and free radical scavenging capacity extensively used medicinal plant of Punica granatum. Asian Journal of Pharmaceutical and Clinical Research. 2016;9(6):140–146. DOI: https://doi.org/10.22159/ajpcr.2016.v9i6.13941.
Bekir J, Mars M, Souchard JP, Bouajila J. Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leaves. Food and Chemical Toxicology. 2013;55:470–475. DOI: https://doi.org/10.1016/j.fct.2013.01.036.
Diallo D, Sanogo R, Yasambou H, Traoré A, Coulibaly K, Maïga A. Étude des constituants des feuilles de Ziziphus mauritiana Lam. (Rhamnaceae), utilisées traditionnellement dans le traitement du diabète au Mali. Comptes Rendus Chimie. 2004;7(10–11):1073–1080. DOI: https://doi.org/10.1016/j.crci.2003.12.035.
Cheurfa M, Allem R. Evaluation of antioxidant activity of different extracts of Aloysia triphylla leaves (L’Herit.) from Algeria in vitro. Phytotherapie. 2016;14(3):181–187. DOI: https://doi.org/10.1007/s10298-015-0969-4.
Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods in Enzymology. 1999;299:152–178. DOI: https://doi.org/10.1016/S0076-6879(99)99017-1.
Mbaebie BO, Edeoga HO, Afolayan AJ. Phytochemical analysis and antioxidants activities of aqueous stem bark extract of Schotia latifolia Jacq. Asian Pacific Journal of Tropical Biomedicine. 2012;2(2):118–24. DOI: https://doi.org/10.1016/S2221-1691(11)60204-9.
Kosalec I, Pepeljnjak S, Bakmaz M, Vladimir-Knezević S. Flavonoid analysis and antimicrobial activity of commercially available propolis products. Acta Pharmaceutica. 2005;55(4):423–230.
Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: Specific application to the determination of vitamin E. Analytical Biochemistry. 1999;269(2):337–341. DOI: https://doi.org/10.1006/abio.1999.4019.
Benzie IFF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: The FRAP assay. Analytical Biochemistry. 1996;239(1):70–76. DOI: https://doi.org/10.1006/abio.1996.0292.
Magalhaes LM, Segundo MA, Reis S, Lima J. Methodological aspects about in vitro evaluation of antioxidant properties. Analytica Chimica Acta. 2008;613(1):1–19. DOI: https://doi.org/10.1016/j.aca.2008.02.047.
Nagulendran K, Velavan S, Mahesh R, Begum VH. In vitro antioxidant activity and total polyphenolic content of Cyperus rotundus rhizomes. Journal of Chemistry. 2007;4(3):440–449. DOI: https://doi.org/10.1155/2007/903496.
Brand-Williams W, Cuvelier ME, Berset C. Use of a free-radical method to evaluate antioxidant activity. LWT – Food Science and Technology. 1995;28(1):25–30.
Daksha G, Subraya Chandrashekar K, and Pai G. In vitro antidiabetic activity of pentacyclic tritrpenoids and fatty acid esters from Bauhinia Purpurea. International Journal of Pharmacology and Pharmaceutical Technology. 2013;2(1):25–28.
Nair SS, Kavrekar V, Mishra A. Evaluation of in vitro anti diabetic activity of selected plant extracts. International Journal of Pharmaceutical Science Invention. 2013;2(4):12–19.
Sinha SK, Ahmad I, Gayathri M. Antidiabetic effect of ethanol extract of Syzygium jambolanum seed (in-vitro). International Journal of Drug Development and Research. 2013;5(3):187–191.
Ukwubile CA, Odugu JA. Evaluation of antibacterial and in vitro antidiabetic activities of Phyllanthus amarus Linn. (Phyllanthaceae) leaf ethanol extract. Journal of Bacteriology and Mycology: Open Access. 2018;6(4):254–256. DOI: https://doi.org/10.15406/jbmoa.2018.06.00214.
Elfalleh W, Hannachi H, Tlili N, Yahia Y, Nasri N, Ferchichi A. Total phenolic contents and antioxidant activities of pomegranate peel, seed, leaf and flower. Journal of Medicinal Plants Research. 2012;6(32):4724–4730. DOI: https://doi.org/10.5897/JMPR11.995.
Dasgupta N, De B. Antioxidant activity of Piper betle L. leaf extract in vitro. Food Chemistry. 2004;88(2):219–224. DOI: https://doi.org/10.1016/j.foodchem.2004.01.036.
Kam A, Li KM, Razmovski-Naumovski V, Nammi S, Shi J, Chan K, et al. A comparative study on the inhibitory effects of different parts and chemical constituents of pomegranate on α-amylase and α-glucosidase. Phytotherapy Research. 2013;27(11):1614–1620. DOI: https://doi.org/10.1002/ptr.4913.
Oleynikov VV. Antioxidant and antimicrobial properties of oregano extract (Origani vulgaris herba L.). Foods and Raw Materials. 2020;8(1):84–90. DOI: https://doi.org/10.21603/2308-4057-2020-1-84-90.
Pennington JAT, Fisher RA. Classification of fruits and vegetables. Journal of Food Composition and Analysis. 2009;22:S23–S31. DOI: https://doi.org/10.1016/j.jfca.2008.11.012.
Laughton MJ, Halliwell B, Evans PJ, Hoult JRS. Antioxidant and pro-oxidant actions of the plant phenolics quercetin, gossypol and myricetin: Effects on lipid-peroxidation, hydroxyl radical generation and bleomycin-dependent damage to DNA. Biochemical Pharmacology. 1989;38(17):2859–2865. DOI: https://doi.org/10.1016/0006-2952(89)90442-5.
Apak R, Guclu K, Demirata B, Ozyurek M, Celik SE, Bektasoglu B, et al. Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules. 2007;12(7):1496–1547. DOI: https://doi.org/10.3390/12071496.
Perron NR, Brumaghim JL. A review of the antioxidant mechanisms of polyphenol compounds related to iron binding. Cell Biochemistry and Biophysics. 2009;53(2):75–100. DOI: https://doi.org/10.1007/s12013-009-9043-x.
Nkhili E. Polyphénols de l’Alimentation: Extraction, Interactions avec les ions du Fer et du Cuivre, Oxydation et Pouvoir antioxidant [Polyphenols from the feed: Extraction, Interactions with iron and copper ions, oxidation and antioxidant power]. Dr. eng. sci. diss. Montpellier: Université D’Avignon; 2009. 378 p. (In French).
Crozier A, Del Rio D, Clifford MN. Bioavailability of dietary flavonoids and phenolic compounds. Molecular Aspects of Medicine. 2010;31(6):446–467. DOI: https://doi.org/10.1016/j.mam.2010.09.007.
Manach C, Mazur A, Scalbert A. Polyphenols and prevention of cardiovascular diseases. Current Opinion in Lipidology. 2005;16(1):77–84. DOI: https://doi.org/10.1097/00041433-200502000-00013.
Han XZ, Shen T, Lou HX. Dietary polyphenols and their biological significance. International Journal of Molecular Sciences. 2007;8(9):950–988. DOI: https://doi.org/10.3390/i8090950.
Hodgson JM, Croft KD. Tea flavonoids and cardiovascular health. Molecular Aspects of Medicine. 2010;31(6):495–502. DOI: https://doi.org/10.1016/j.mam.2010.09.004.
Cotelle N. Role of flavonoids in oxidative stress. Current Topics in Medicinal Chemistry. 2001;1(6):569–590. DOI: https://doi.org/10.2174/1568026013394750.
Kang DG, Yun CK, Lee HS. Screening and comparison of antioxidant activity of solvent extracts of herbal medicines used in Korea. Journal of Ethnopharmacology. 2003;87(2–3):231–236. DOI: https://doi.org/10.1016/s0378-8741(03)00142-9.
Patel AN, Bandawane DD, Mhetre NK. Pomegranate (Punica granatum Linn.) leaves attenuate disturbed glucose homeostasis and hyperglycemia mediated hyperlipidemia and oxidative stress in streptozotocin induced diabetic rats. European Journal of Integrative Medicine. 2014;6(3):307–321. DOI: https://doi.org/10.1016/j.eujim.2014.03.009.
Thilakarathna SH, Rupasinghe HPV. Flavonoid bioavailability and attempts for bioavailability enhancement. Nutrients. 2013;5(9):3367–3387. DOI: https://doi.org/10.3390/nu5093367.
Takahama U, Hirota S. Interactions of flavonoids with α-amylase and starch slowing down its digestion. Food and Function. 2018;9(2):677–687. DOI: https://doi.org/10.1039/c7fo01539a.
Ahmed D, Kumar V, Sharma M, Verma A. Target guided isolation, in-vitro antidiabetic, antioxidant activity and molecular docking studies of some flavonoids from Albizzia Lebbeck Benth. bark. BMC Complementary and Alternative Medicine. 2014;14. DOI: https://doi.org/10.1186/1472-6882-14-155.
Abirami N, Natarajan B, Sagadevan E. Phytochemical investigation and in vitro evaluation of hypoglycemic potential of Grewia hirsute. International Journal of Pharma and Bio Sciences. 2014;5(1):76–83.
Kwon O, Eck P, Chen SL, Corpe CP, Lee JH, Kruhlak M, et al. Inhibition of the intestinal glucose transporter GLUT2 by flavonoids. Faseb Journal. 2007;21(2):366–377. DOI: https://doi.org/10.1096/fj.06-6620com.
Chakraborty DP. Chapter 4. Chemistry and biology of carbazole alkaloids. The Alkaloids: Chemistry and Pharmacology. 1993;44:257–364. DOI: https://doi.org/10.1016/s0099-9598(08)60146-7.

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Cheurfa M, Achouche M, Azouzi A, Abdalbasit MA. Antioxidant and anti-diabetic activity of pomegranate (Punica granatum L.) leaves extracts. Foods and Raw Materials. 2020;8(2):329–336. DOI: http://doi.org/10.21603/2308-4057-2020-2-329-336

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