Abstract

In this study, we applied the D-optimal mixture design method to optimise prebiotic sausage formulation with inulin, konjac (Amorphophallus konjac L.), and starch. Also, we investigated the effect of each component indi- vidually as well as their mixtures on cooking characteristics, texture, colour and sensory properties of prebiotic sau- sages. The results of this study revealed that the increase in inulin content in the formulations of sausages led to lower frying loss, and increased water holding capacity (WHC), lightness, and overall acceptability. The incorporation of konjac increased the cooking yield, hardness, cohesiveness, redness, and yellowness. On the other hand, konjac added into the sausage formulation decreased overall acceptability. The mixtures of inulin, konjac, and starch improved the cooking characteristics and overall acceptability of the sausages without significant negative effect on the color or sensory properties. The results of the study clarified that the optimum amounts of inulin, starch, and konjac were 2.09; 2.76; and 0.146 %, respectively. The obtained results make it possible to use the combination of these components to produce prebiotic sausage.

Keywords

Inulin, konjac, sausage, functional, formulation

REFERENCES

1. Afshari R., Hosseini H., Khaksar R., et al. Investigation of the Effects of Inulin and β-Glucan on the Physical and Sensory Properties of Low-Fat Beef Burgers Containing Vegetable Oils: Optimisation of the Formulation Using D-Optimal Mixture Design. Food Technology and Biotechnology, 2015, vol. 53, no. 4, pp. 436–445. DOI: https://doi. org/10.17113/ftb.53.04.15.3980.
2. Akesowan A. Effects of sodium chloride, soy protein isolate and secondary gums on Konjac gel properties. An- nals of Biological Research, 2011, vol. 2, no. 1, pp. 181–186.
3. Álvarez D. and Barbut S. Effect of inulin, β-Glucan and their mixtures on emulsion stability, color and textural parameters of cooked meat batters. Meat Science, 2013, vol. 94, no. 3, pp. 320–327. DOI: https://doi.org/10.1016/j. meatsci.2013.02.011.
4. Amini S.R., Hosseini H., Bondarianzadeh D., Jiménez Colmenero F., and Khaksar R. Optimization of prebiotic sausage formulation: Effect of using β-glucan and resistant starch by D-optimal mixture design approach. LWT – Food Science and Technology, 2015, vol. 62, no. 1, pp.704–710. DOI: https://doi.org/10.1016/j.lwt.2014.05.014.
5. Asgharzadeh A., Shabanpour B., Aubourg S.P., and Hosseini H. Chemical changes in silver carp (Hypophthal- michthys molitrix) minced muscle during frozen storage: Effect of a previous washing process. Journal of Grasas y aceites (Sevilla), 2010, vol. 61, no. 1, pp. 95–101. DOI: https://doi.org/10.3989/gya.087109.
6. Bengtsson H., Montelius C., and Tornberg E. Heat-treated and homogenized potato pulp suspensions as additives in low-fat sausages. Meat Science, 2011, vol. 88, no.1, pp.75–81. DOI: https://doi.org/10.1016/j.meatsci.2010.12.005.
7. Bourne M.C. Texture profile analysis. Food Technology, 1978, vol. 32, no. 7, pp. 62–66.
8. Chin K.B., Keeton J.T., Longnecker M.T., and Lamkey J.W. Low-fat bologna in a model system with vary- ing types and levels of konjac blends. Journal of Food Science, 1998, vol. 63, no. 5, pp. 808–813. DOI: https://doi. org/10.1111/j.1365-2621.1998.tb17905.x.
9. Chin K.B., Keeton J.T., Miller R.K., Longnecker M.T., and Lamkey J.W. Evaluation of konjac blends and soy protein isolate as fat replacements in low-fat bologna. Journal of Food Science, 2000, vol. 65, no. 5, pp. 756−763. DOI: https://doi.org/10.1111/j.1365-2621.2000.tb13582.x.
10. Delgado-Pando G., Cofrades S., Rodríguez-Salas L., and Jiménez-Colmenero F. A healthier oil combination and konjac gel as functional ingredients in low-fat pork liver pâté. Meat Science, 2011, vol. 88, no. 2, pp. 241–248. DOI: https://doi.org/10.1016/j.meatsci.2010.12.028.
11. Emir J., Wang, H.X., Amadou I., and Qin X.J. Textural and rheological properties of hydrolyzed Konjac Glu- comannan and Kappa-Carrageenan: Effect of molecular weight, total content, pH and temperature on the mixed system gels. Food Agriculture, 2012, vol. 24, no. 3, pp. 200–207.
12. Holzapfel W.H. and Schillinger U. Introduction to pre-and probiotics. Food Research International, 2002, vol. 35, no. 2–3, pp. 109–116. DOI: https://doi.org/10.1016/S0963-9969(01)00171-5.
13. Hu Y., Liang H., Xu W., et al. Synergistic effects of small amounts of konjac glucomannan on functional prop- erties of egg white protein. Food Hydrocolloids, 2016, vol. 52, pp. 213–220. DOI: https://doi.org/10.1016/j.food- hyd.2015.07.001.
14. Jiménez-Colmenero F., Cofrades S., López-López I., et al. Technological and sensory characteristics of reduced/ low-fat, low-salt frankfurters as affected by the addition of konjac and seaweed. Meat Science, 2010, vol. 84, no. 3, pp. 356–363. DOI: https://doi.org/10.1016/j.meatsci.2009.09.002.
15. Lin K.W. and Huang H.Y. Konjac/gellan gum mixed gels improve the quality of reduced-fat frankfurters. Meat Science, 2003, vol. 65, no. 2, pp. 749–755. DOI: https://doi.org/10.1016/S0309-1740(02)00277-2.
16. Liu J.J., Zhu K.K., Ye T., et al. Influence of konjac glucomannan on gelling properties and water state in egg white protein gel. Food Research International, 2013, vol. 51, no. 2, pp. 437–443. DOI: https://doi.org/10.1016/j. foodres.2013.01.002.
17. López-López I., Cofrades S., and Jiménez-Colmenero F. Low-fat frankfurters enriched with n-3 PUFA and ed- ible seaweed: Effects of olive oil and chilled storage on physicochemical, sensory and microbial characteristics. Meat Science, 2009, vol. 83, no. 1, pp. 148–154. DOI: https://doi.org/10.1016/j.meatsci.2009.04.014.
18. Méndez-Zamora G., García-Macías J.A., Santellano-Estrada E., et al. Fat reduction in the formulation of frank- furter sausages using inulin and pectin. Food Science and Technology (Campinas), 2015, vol. 35, no. 1, pp. 25–31. DOI: https://doi.org/10.1590/1678-457X.6417.
19. Menegas L.Z., Pimentel T.C., Garcia S., and Prudencio S.H. Dry-fermented chicken sausage produced with in- ulin and corn oil: physicochemical, microbiological, and textural characteristics and acceptability during storage. Meat Science, 2013, vol. 93, no. 3, pp. 501–506. DOI: https://doi.org/10.1016/j.meatsci.2012.11.003.
20. Nowak B., Von Mueffling T., Grotheer J., Klein G., and Watkinson B.M. Energy content, sensory properties, and microbiological shelf life of German bologna-type sausages produced with citrate or phosphate and with inulin as fat replacer. Journal of Food Science, 2007, vol. 72, no. 9, pp. S629–S638. DOI: https://doi.org/10.1111/j.1750- 3841.2007.00566.x.
21. Purwandari U., Khoiri A., Muchlis M., et al. Textural, cooking quality, and sensory evaluation of gluten-free noodle made from breadfruit, konjac, or pumpkin flour. International Food Research Journal, 2014, vol. 21, no. 4, pp. 1623–1627.
22. Ruiz-Capillas C., Triki M., Herrero A.M., Rodriguez-Salas L., and Jiménez-Colmenero F. Konjac gel as pork backfat replacer in dry fermented sausages: Processing and quality characteristics. Meat Science, 2012, vol. 92, no. 2, pp. 144–150. DOI: https://doi.org/10.1016/j.meatsci.2012.04.028.
23. Savadkoohi S., Hoogenkamp H., Shamsi K., and Farahnaky A. Color, sensory and textural attributes of beef frankfurter, beef ham and meat-free sausage containing tomato pomace. Meat Science, 2014, vol. 97, no. 4, pp. 410–418. DOI: https://doi.org/10.1016/j.meatsci.2014.03.017.
24. Trespalacios P. and Pla R. Simultaneous application of transglutaminase and high pressure to improve functional properties of chicken meat gels. Food Chemistry, 2007, vol. 100, no. 1, pp. 264–272. DOI: https://doi.org/10.1016/j. foodchem.2005.09.058.
25. Yang D., Yuan Y., Wang L., et al. A Review on Konjac Glucomannan Gels: Microstructure and Application.International Journal of Molecular Sciences, 2017, vol. 18, no. 11. DOI: https://doi.org/10.3390/ijms18112250.