ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Theory and Practice of Prion Protein Analysis in Food Products

The article presents the results of the research on methods of identification and quantitative determination of prion proteins in biological samples and multicomponent mixtures based on them. Analysis of nucleotide sequence of DNA encoding the PRNP gene of the prion protein, including phylogenetic and comparative analysis of nucleotide sequences of normal and pathogenic prion protein in cattle, was performed. Oligonucleotide primers for amplification of the PRNP gene of pathogenic prion protein were designed and synthesized. The high specificity of the developed test system was confirmed.
Ключевые слова
prion , protein , encephalopathy , safety , quality , PCR , analysis
  1. Wickner, R.B., Edskes, H.K., Roberts, B.T., Baxa, U., Pierce, M.M., Ross, E.D., and Brachmann, A., Prions: Proteins as Genes and Infectious Entities, Genes Dev., 2004. V. 18, no. 5, P. 470–485.
  2. Heikenwalder, M., Julius, C., Aguzzi, A., Prions and Peripheral Nerves: A Deadly Rendezvous, J. Neurosci., 2007. V. 85, P. 2714–2725.
  3. Weissmann, C., and Aguzzi, A., Approaches to Therapy of Prion Diseases, Annu. Rev. Med., 2005. V. 56, P. 321–344.
  4. Bartz, J.C., Dejoia, C., Tucker, T., Kincaid, A.E., and Bessen, R.A., Extraneural Prion Neuroinvasion without Lymphoreticular System Infection, J. Virol., 2005. V. 79, P. 11858–11863.
  5. Vana, K., Zuber, C., Nikles, D., and Weiss, S., Novel Aspects of Prions, Their Receptor Molecules, and Innovative Approaches for TSE Therapy, Cell. Mol. Neurobiol., 2007. V. 27, no. 1, P. 107–128.
  6. Elementnyi ekspress analizator Rapid N cube. Kratkoe rukovodstvo (Express elemental analyzer Rapid N-cube. Brief manual) (Moscow, 2009).
  7. Prosekov, A.Yu., Babich, O.O., and Sukhikh, S.A., Sovremennye metody issledovaniya syr’ya i biotekhnologicheskoi produktsyi: Laboratornyi praktikum (Modern methods of investigation of raw materials and biotechnology products: Laboratory course), (Kemerovo Institute of Food Science and Technology, Kemerovo, 2012).
  9. Prusiner, S.B., Prions, Proc. Natl. Acad. Sci. USA, 1998. V. 95, no. 23, P. 13363–13383.
  10. Apetri, A.C., and Surewicz, A.C., Kinetic Intermediate in the Folding of Human Prion Protein, J. Biol. Chem., 2002. V. 277, P. 44589–44592.
  11. Patrushev, L.I., Iskusstvennye geneticheskie sistemy. T. 1. Gennaya i belkovaya inzheneriya (Artificial genetic systems. V. 1. Gene and protein engineering) (Nauka, Moscow, 2004).
  12. Aguzzi, A., Sigurdson, C., Heikenwalder, M., Molecular Mechanisms of Prion Pathogenesis, Annu. Rev. Pathol. Mech. Dis., 2008. V. 3, P. 11–40.
Как цитировать?
Theory and Practice of Prion Protein Analysis in Food Products. Foods and Raw Materials, 2014, vol. 2, no. 2, pp. 106-120
Кемеровский государственный университет
2308-4057 (Print) /
2310-9599 (Online)
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