АннотацияIntroduction. Osteoporosis is one of the most common diseases of the musculoskeletal system in modern clinical practice. Its prevention and treatment requires a diet with a sufficient intake of calcium, vitamins, and connective tissue proteins that regenerate cartilage and bone tissue. We aimed to formulate a functional product based on collagen fermentolysate to prevent osteoporosis and prove its effects in experiments on laboratory rats.
Study objects and methods. Our study objects were collagen fermentolysate obtained from pork ears and legs (1:1) and the functional product based on it. The biological experiment was carried out on Wistar female rats exposed to osteoporosis through complete ovariectomy. Their femurs were analyzed for the contents of phosphorus, magnesium, and calcium, as well as cytometric and biochemical blood parameters.
Results and discussion. The formulated functional product based on collagen fermentolysate contained 41% of the most easily assimilable peptide fractions with a low molecular weight of 10 to 20 kDa. Other components included pumpkin powder, dietary fiber, calcium, chondroprotectors, and vitamins E, C, and D3. Compared to the control, the experimental rats that received the functional product had increased contents of calcium and magnesium in the bone tissue (by 25.0 and 3.0%, respectively), a decreased content of phosphorus (by 7.0%), a calcium-to-phosphorus ratio restored to 2.4:1.0, and a higher concentration of osteocalcin in the blood serum (by 15%).
Conclusion. The developed functional product based on collagen fermentolysate can be used as an additional source of connective tissue protein, calcium, vitamins C, E, and D3, dietary fiber, and chondroprotectors to prevent osteoporosis.
Ключевые словаCollagen , fermentolysate , osteoporosis , functional foods , raw meat , calcium , oophorectomy
- Pisani P, Renna MD, Conversano F, Casciaro E, Di Paola M, Quarta E, et al. Major osteoporotic fragility fractures: Risk factor updates and societal impact. World Journal of Orthopedics. 2016;7(3):171–181. https://doi.org/10.5312/wjo.v7.i3.171.
- Srinivasan V, Martens MG. Hormone therapy in menopausal women with fibroids: is it safe?. Menopause. 2018;25(8):930–936. https://doi.org/10.1097/gme.0000000000001105.
- Muhammad A, Mada SB, Malami I, Forcados GE, Erukainure OL, Sani H, et al. Postmenopausal osteoporosis and breast cancer: The biochemical links and beneficial effects of functional foods. Biomedicine and Pharmacotherapy. 2018;107:571–582. https://doi.org/10.1016/j.biopha.2018.08.018.
- Rajput R, Wairkar S, Gaud R. Nutraceuticals for better management of osteoporosis: An overview. Journal of Functional Foods. 2018;47:480–490. https://doi.org/10.1016/j.jff.2018.06.013.
- Pinkerton JV, Aguirre FS, Blake J, Cosman F, Hodis H, Hoffstetter S, et al. The 2017 hormone therapy position statement of the North American Menopause Society. Menopause. 2017;24(7):728–753. https://doi.org/10.1097/gme.0000000000000921.
- Belyaev NG, Timchenko LD, Rzhepakovsky IV, Piskov SI, Lodygin AD, Gaponov VI, et al. Osteoprotective effect of bread enriched with protein, dietary fiber, calcium, iron and iodine in hypoestrogen-induced osteoporosis among rats. Problems of Nutrition. 2020;89(6):58–69. (In Russ.). https://doi.org/10.24411/0042-8833-2020-10079.
- Liu J, Liu J, Liu L, Zhang G, Zhou A, Peng X. The gut microbiota alteration and the key bacteria in Astragalus polysaccharides (APS)-improved osteoporosis. Food Research International. 2020;138. https://doi.org/10.1016/j.foodres.2020.109811.
- Honvo G, Lengelé L, Charles A, Reginster J-Y, Bruyère O. Role of collagen derivatives in osteoarthritis and cartilage repair: A systematic scoping review with evidence mapping. Rheumatology and Therapy. 2020;7:703–740. https://doi.org/10.1007/s40744-020-00240-5.
- Kakimov A, Suychinov A, Mayorov A, Yessimbekov Z, Okuskhanova E, Kuderinova N, et al. Meat-bone paste as an ingredient for meat batter, effect on physicochemical properties and amino acid composition. Pakistan Journal of Nutrition. 2017;16(10):797–804. https://doi.org/10.3923/pjn.2017.797.804.
- Nasri M. Protein hydrolysates and biopeptides: Production, biological activity, and application in food and health benefits. A review. Advances in Food and Nutrition Research. 2018;81:109–159. https://doi.org/10.1016/bs.afnr.2016.10.003.
- Kiewiet MBG, Faas MM, de Vos P. Immunomodulatory protein hydrolysates and their application. Nutrients. 2018;10(7). https://doi.org/10.3390/nu10070904.
- López-Pedrouso M, Borrajo P, Pateiro M, Lorenzo JM, Franco D. Antioxidant activity and peptidomic analysis of porcine liver hydrolysates using alcalase, bromelain, flavourzyme and papain enzymes. Food Research International. 2020;137. https://doi.org/10.1016/j.foodres.2020.109389.
- Inoue N, Sugihara F, Wang X. Ingestion of bioactive collagen hydrolysates enhance facial skin moisture and elasticity and reduce facial ageing signs in a randomised double-blind placebo-controlled clinical study. Journal of the Science of Food and Agriculture. 2016;96(12):4077–4081. https://doi.org/10.1002/jsfa.7606.
- Shi P, Liu M, Fan F, Chen H, Yu C, Lu W, et al. Identification and mechanism of peptides with activity promoting osteoblast proliferation from bovine lactoferrin. Food Bioscience. 2018;22:19–25. https://doi.org/10.1016/j.fbio.2017.12.011.
- Hong H, Fan H, Chalamaiah M, Wu J. Preparation of low-molecular-weight, collagen hydrolysates (peptides): Current progress, challenges, and future perspectives. Food Chemistry. 2019;301. https://doi.org/10.1016/j.foodchem.2019.125222.
- Yunusov EhSh, Ponomarev VYa, Morozova SA, Ezhkova GO. Izuchenie gidroliza kollagensoderzhashchego syrʹya proteoliticheskimi fermentami [A study of hydrolysis of collagen-containing materials with proteolytic enzymes]. Bulletin of the Technological University. 2016;19(24):168–170. (In Russ.).
- Aslanova MA, Dydykin AS, Soldatova NE. Preparation of protein hydrolyzate from raw materials of animal origin for the enrichment of products. Food Industry. 2018;(2):16–18. (In Russ.).
- Chernukha IM, Kotenkova EA, Vasilevskaya ER, Ivankin AN, Lisitsyn AB, Fedulova LV. The study of biological effects of different geographical origin goji berries in rats with alimentary hypercholesterolemia. Problems of Nutrition. 2020;89(1):37–45. (In Russ.). https://doi.org/10.24411/0042-8833-2020-10004.
- Nikolaeva TI, Shekhovtsov PV. Hydrolysates of collagen concerning prevention and healing joint diseases. Fundamental research. 2014;(12–3):524–528. (In Russ.).
- Marchenkova LA, Fesyun AD, Gerasimenko MYu, Makarova EV. The effect of administration of dietary supplement with calcium and vitamins D3 and B6 on calcium homeostasis and falls incidence in patients with high fracture risk undergoing medical rehabilitation. Problems of Nutrition. 2020;89(5):89–100. (In Russ.). https://doi.org/10.24411/0042-8833-2020-10069.
- Heaney RP, Bone as the calcium nutrient reserve. In: Weaver CM, Heaney RP, editors. Calcium in human health. Totowa, New Jersey: Humana Press; 2006. pp. 7–12. https://doi.org/10.1007/978-1-59259-961-5_2.
- Scholz-Ahrens KE, Schaafsma G, Van den Heuvel EGHM, Schrezenmeir J. Effects of prebiotics on mineral metabolism. The American Journal of Clinical Nutrition. 2001;73(2):459S–464S. https://doi.org/10.1093/ajcn/73.2.459s.
- Puzikov AM. Osteoporosis correction in experiment. Experimental and Clinical Gastroenterology Journal. 2014;102(2):25–31. (In Russ.).
- Klimova ZhA, Zaft AA, Zaft VB. Modern laboratory diagnosis of osteoporosis. International Journal of Endocrinology. 2014;63(7):75–84. (In Russ.).