АннотацияIntroduction. After the Iraq war, the dust phenomenon has increased in western Iran. Our study aimed to evaluate the effect of the dust phenomenon on the content of heavy metals in raw milk in Ilam province.
Study objects and methods. The dust samples were collected during one year. The concentrations of dust particles were determined with the Enviro Check Laser System, using the Dust Monitor Check. The concentration of heavy metals in dust was determined by using the high volume air samplers and glass fiber filters.
Results and discussion. Heavy metals (lead, arsenic, zinc, copper, and iron) were measured at four sampling sites in raw milk by the atomic absorption method. The mean and standard deviations of dust particulate matter (PM10 and PM2.5) were 105.6 ± 90.5 and 25.9 ± 15.4 μg/m3, respectively. The amounts of arsenic, zinc, lead, and copper were higher in the spring and summer. Lead levels in western and southern regions were higher than those in the east, center, and north.
Conclusion. We found similar trends for arsenic, zinc, copper, and iron in raw milk. Our results showed the potential effect of the dust phenomenon on the presence of heavy metals in raw milk.
Ключевые словаHeavy metal , dust phenomena , raw milk , atomic absorption
ВВЕДЕНИЕA dust storm is one of many air pollutants known to humans. About 800 trillion grams of dust particles are spread in Asia. Particulate matter (PM) is usually suspended in arid, semiarid, and desert areas . Today, dust storms are one of environmental problems that threaten the world . The phenomenon of dust in the atmosphere causes the spread of PM around the globe . Dust phenomena can be triggered by such factors as environmental change, global drought, and land cover . The dry currents of the Saudi air and lack of attention to the environment and desertification in Iraq have dried up many of the marshes there and created dusty areas. In the past, Iran, Iraq, and Saudi Arabia jointly funded the mulching of these lands during a particular season. The Iraq war obliterated this work, resulting in a spike in western Iran, and eventually almost throughout the whole country. According to Harrington et al., the United States soldiers involved in the Iraq war had respiratory problems due to the dust phenomenon in that country .
Biological particles, salt sprays and, in particular, dust phenomena have been reported to contain numerous heavy metals . Furthermore, these metals lead to climate change in temperature and other seasonal changes, such as the wind speed and patterns . Although trace heavy metals are fundamental to living organisms for a normal and healthy life, excessive levels of heavy metal contamination in the environment could cause harm [8, 9]. To reduce environmental pollution and mitigate the resulting degradation of soil and water resources, it is important to precisely determine heavy metal concentrations [10, 11].
Some heavy metals, for example, chrome (Cr), lead (Pb), cadmium (Cd), and mercury (Hg) in the form of suspended particles in the air produce significant toxicological effects on people and other organisms by contaminating food and drinking water in the environment . Particulate matter, which is contaminated with heavy metals, can pollute groundwater. It has been indicated that contamination is transmitted from the soil to the plant. As a result, contaminated plants ingested by humans or animals can cause a toxic effect. Consequently, the concentration of heavy metals in animal products, such as raw milk, also causes toxicity. The extent of toxicity depends on different factors, such as plant processes and the amount of raw materials used . Moreover, heavy metals such as lead, cadmium, chrome, nickel, and cobalt can contaminate cows and their surroundings. Heavy metals are absorbed by plant roots from the soil. As a result, this pollution causes serious problems, changing the amount and structure of milk .
The International Agency for Research on Cancer (IARC, 2016) classifies arsenic (As) and chrome (Cr) as carcinogenic metals and lead as a possible carcinogen. Such metals can cause different types of cancer through dermal contact, inhalation, and ingestion .
The western and southwestern climates of Iran are influenced by environmental conditions and markers such as geological, climatic, hydrological, and geomorphological characteristics . Among the factors that cause dust storms are the development of deserts in Iraq, a decrease in volume and flow of rivers, and the Turkish dam on the rivers . With a population of 172 000 people, the city of Ilam is located on the western border of Iran (33°38′N, 46°25′E). Due to a small population and a mountainous area, the city has low traffic and also no air pollution industry.
With the onset of spring, the phenomenon of dust comes to Iran from Iraq. This situation was aggravated by the Iraq war. In this study, we investigated the concentration of suspended particles in the environment and evaluated the effect of heavy metals on cow raw milk in the west of the country, Ilam province.
ОБЪЕКТЫ И МЕТОДЫ ИССЛЕДОВАНИЯPM concentration in dust. The dust samples were collected from four districts of Ilam province: northern and central, southern, western, and eastern (28 samples in each). The sampling was performed on the roofs of buildings seven meters above the ground and two meters from the roof surface. During the sampling, we complied with all the standards of the US Environmental Protection Agency. In particular, we kept the required distance from natural and artificial obstacles, pathways, and sources of contamination. Suspended particles PM10 and PM2.5 were measured with the Enviro Check Laser System using the Dust Monitor Check (Grimm). This apparatus can directly and simultaneously measure the particle count, PM10 and PM2.5. The system automatically saves the values in its memory and calculates the average on an hourly and a daily basis .
On normal days, the sampling was carried out every six days and on days with dust (a concentration above 150 μg/cm3), according to the Meteorological Organization and satellite sites, on a daily basis. The peak concentrations of PM10 a nd PM2.5 were recorded and measured on average every hour. The data obtained during a year were analyzed with the SPSS software .
Heavy metals in dust. Teflon and fiberglass filters were used to investigate heavy metals in the dust phenomenon. A 100-cm section of a filter was cut and transferred to a 100 mL beaker. Then, we added 50 mL of Aqua Regia (HNO3+ 3 HCl) and heated it to 140°C until the filter section was dry. Then, we removed it and washed the beaker with 10% nitric acid. This work was repeated three times. In the end, the prepared sample was kept at room temperature until it was cooled. Then, we transferred it into a 100-mL volumetric flask and diluted to volume with 10% HNO3. The concentrations of heavy metals (lead, arsenic, zinc, copper, and iron) were determined by a Perkin-Elmer Analyst 800 atomic absorption spectrometer, including an AS-800 Autosampler equipped with Zeeman-effect background correction. Each result was an average of three readings. Blank filters were prepared by digesting clean glass fiber filters with the same digestion method used for the dust samples. Also, the dust samples were prepared in different seasons .
Heavy metals in raw milk. A total of 112 samples of cow raw milk were collected from four districts of Ilam province: northern and central, southern, western, and eastern (28 in each), at the same places as dust samples. All the samples were collected in nitric acidwashed polyethylene containers. They were immediately transported to the laboratory in a cooler with ice packs and stored at –20°C until analysis. The raw milk samples were analyzed based on AOAC official methods. The amounts of heavy metals (lead, arsenic, zinc, copper, and iron) were measured by a Perkin-Elmer Analyst 800 atomic absorption spectrometer, including an AS-800 Autosampler equipped with Zeeman-effect background correction. Each sample was studied three times . The limit of detection and the limit of quantitation of the atomic absorption device were 0.08 ppm and 0.15 ppm, respectively.
Statistical analysis. The SPSS 21 software was used to extract the data (P < 0.05). The results of three repetitions were analyzed by ANOVA, using an SPSS statistics package.
РЕЗУЛЬТАТЫ И ИХ ОБСУЖДЕНИЕ
Ilam is bordered by Iraq and close to the countries of Saudi Arabia and Kuwait, which are the main sources of dust events in the Middle East (Fig. 1).
According to Table 1, the sampling took 87 days. The average PM10 and PM2.5 were about 105.6 ± 82.9 and 25.9 ± 15.4 μg/m3, respectively. The maximum particle size of PM10 and PM2.5 was about 806.3 and 213.2 μg/m3, respectively.
Five heavy metals (lead, arsenic, zinc, copper, and iron) were measured in all the samples. Figure 2 shows the range and mean concentrations (ng/m3) of the selected heavy metals analyzed at the sampling stations. In the spring and summer, the amounts of heavy metals were higher than in the other seasons, especially iron. Lead and copper levels were lower in all the seasons compared to other metals.
Average values of iead content in the raw milk samples are shown in Table 2. The highest average lead level was determined in the western region (57.1 μg/kg). The statistical analysis revealed a significant difference in lead concentrations between the western and southern regions compared to the east and north of Ilam province (P-value < 0.05).
Average concentrations of arsenic in the milk samples are shown in Table 3. Although arsenic was higher in the south and west compared to the northern and central region or the east of the province, we found no significant difference. The highest average arsenic level was observed in the west, amounting to 12.4 mg/kg.
Table 4 shows average zinc values in the raw milk samples. The highest average amount of zinc was determined in the western region (4582.8 μg/kg). According to the statistical analysis, there was a significant difference in zinc concentrations between the western and southern regions compared to the east and north of Ilam province (P-value < 0.05).
The concentrations of copper in different regions are shown in Table 5. As we can see, the average level of copper in the western and southern regions was higher than in the eastern and northern regions. The lowest average copper concentration (234.3 μg/kg) was observed in the northern and central region.
According to Table 6, there was a significant difference between the amounts of iron in the west and south compared to the east and north of the province. The highest average concentration of iron (3954 μg/kg) was found in the southern region.
Typically, dust particles with a diameter of 616–660 μm remain in their places of origin. Particles sized 31‒62 μm are dispersed over approximately 320 km from their origin, while those sized 16‒30 μm, up to 1600 kilometers. Particles below 16 μm travel longer, and particles ranging from 2‒50 μm have been reported to mostly originate in deserts like Iraq, Saudi Arabia, and Africa . The main source of dust phenomenon in the southwest of Iran is the deserts of Iraq .
In our study, the mean and standard deviations of PM10 and PM2.5 were 105.69 ± 0.5 and 25.9 ± 15.4 μg/m3 at the time of sampling, and the maximum PM10 and PM2.5 concentrations were 806.3 and 213.2 μg/m3 in June, respectively. According to Draxler et al., the main sources of dust phenomenon in the southwest and west of Iran are Kuwait, Iraq, and Saudi Arabia. The authors showed that PM10concentrations exceeded 3000 μg/m3 . In the study by Shahsavani et al., which agrees with our results, the mean and standard deviations of PM10 and PM2.5 in Ahwaz, southwest of Iran, were 407.07 ± 319.1 and 83.2 ± 69.5 μg/m3, while the maximum PM10 and PM2.5 concentrations were 5337.6 and 910.9 μg/m3 in June, respectively . In another study, in the southwest of Iran, the mean and standard PM10 and PM2.5 deviations in the entire study period were 775.3 ± 598.9 and 129.5 ± 114.9 μg/m3, whereas their maximum concentrations reached 4730.1 and 774.4 μg/m3 in February, respectively . The difference between the maximum concentrations found by the authors and our results may be due to differences in geographical and atmospheric conditions and the distance from the dust source in the period of particle measurement. Also, in another study of 2007, the mean total concentration of suspended particles was 282 μg/m3 and the PM10 and PM2.5 concentrations were 165 and 67 μg/m3, respectively . Finally, a 2010 study conducted in China reported the mean concentrations of PM10 and PM2.5 to reach 322 ± 237.4 μg/m3 and 141.5 ± 108.8 μg/m3, respectively .
According to Jacobs et al., of 16.4 million homes in the United States with more than one child below six, 25% still had significant amounts of lead-contaminated deteriorated paint, dust, or adjacent bare soil . Lu et al. detected the presence of heavy metals in soil by spectroscopic methods . In another study, the voltammetric method found cadmium (0.06 μg/L) and lead (0.65 μg/L) in soil . However, the atomic absorption technique is also very important for analyzing heavy metals in air samples. Factors such as high sensitivity, high performance, low cost, and accuracy make this method a good choice. The amount of heavy metals in the atmosphere depends on the origin and the distance from the source of pollution. Seasonal changes also affect concentrations of heavy metals in the atmosphere . Our results showed that the amounts of arsenic, zinc, lead, and copper were higher during the spring and summer. Zinc and iron had a higher level compared to the others. Due to high temperatures during the spring and summer, dust is denser than in the fall or winter, and it is easier for animals and humans to inhale them. In a study by Al-Dabbas et al., the X-ray powder diffraction method was used to detect the presence of heavy metals (Fe, Co, Ni, Cu, Zn, and Pb) and dust particles in the streets of southern Iraq . Also, in Ahwaz, southwest Iran, heavy metals (Cd, Cr, Co, Ni, Pb, Zn, and Al) were identified in particles with PM10 .
It has been well demonstrated that heavy metals such as cadmium, chrome, nickel, and cobalt, which contaminate the environment around animals, e.g. cows, penetrate into cow milk and cause tissue problems. Heavy metals can penetrate into plants through their roots. Through contaminated drinking water and water used in agriculture and food production, they enter animal and human bodies . As reported by Razafsha et al., plants contaminated with particles containing heavy metals increase the risk of raw milk contamination .
Dairy products are a vital part of a healthy diet, and milk is widely used in feeding infants and children. Therefore, studying the presence of metals in milk is especially important to ensure the safety of milk production that is greatly reduced in a contaminated and toxic environment [35, 36].
According to the FAO/WHO guidelines and the Codex standard, the levels of lead, arsenic, zinc, and iron in milk and dairy products are 2.0, 0.050, 0.9, and 0.6 μg/kg, respectively .
We examined the amounts of heavy metals in cow milk in Ilam, west of Iran, and found that lead levels in the western and southern regions were higher than in the east, center, and north. This situation was also consistent with arsenic, zinc, copper, and iron. The average concentrations of arsenic and copper were generally lower than those of zinc, lead, and iron. Farm animals, which are used for milk and meat, tend to get polluted with heavy metals through the environment. According to recent observations, the concentrations of remaining heavy metals in milk are significantly higher than those approved by international authorities .
Consistent with our study, Konuspayeva et al. reported that seasonal changes influenced the level of lead in camel milk: it was lower in the spring compared to other seasons . They also found that the presence of arsenic in camel milk and its contamination level depended on the distance from the source of pollution, wind and farm topography (soil type, vegetation type), etc. . Another study reported that the amount of iron and copper in the milk collected in industrial areas was higher than that of lead in traffic-intensive and industrial regions . This shows the effect of the environment on the content of heavy metals in milk. Awasthi et al. and Malhat et al. reported that the levels of cadmium, iron, and zinc in cow milk were higher in industrial areas compared to others [41, 42]. A study in Egypt found that the amount of cadmium in cow milk produced in contaminated air was significantly higher .
It has been shown that all the milk samples collected from different governorates contained lead and iron in higher concentrations than those recommended for milk by the IDF standard (1979). Lead is an environmental pollutant that is toxic to humans and animals .
Similar to our study, Kabir et al. presented the average concentrations of metals in 50 samples of cow milk from contaminated environments in the following order: Fe > Cr > Mn > Zn > Ni > PB > Hg > Sc > Cd > As .
ВЫВОДЫWe analyzed the particle matter and the amounts of heavy metals in the dust phenomenon in western Iran (Ilam province) over one year and also studied the presence of heavy metals in the raw milk samples collected in its four regions. As a result, we can conclude that the dust phenomenon that comes from Iraq to Iran is probably one of the sources of milk contamination in western Iran.
КОНФЛИКТ ИНТЕРЕСОВThe authors declare that they have no conflicts of interest.
БЛАГОДАРНОСТИThis work was supported by the Ilam University of Medical Sciences.
- Amarloei A, Jonidi JA, Asilian MH, Asadollahi K. The evaluation of PM10, PM2.5 and PM1 concentration during dust storm events in Ilam city, from Mar 2013 through Feb 2014. Journal of Ilam University of Medical Sciences. 2014;22(4):240–259.
- Amiraslani F, Dragovich D. Combating desertification in Iran over the last 50 years: An overview of changing approaches. Journal of Environmental Management. 2011;92(1):1–13. DOI: https://doi.org/10.1016/j.jenvman.2010.08.012.
- Geravandi S, Yari AR, Jafari M, Goudarz G, Vosoughi M, Dastoorpoor M, et al. Effects of dust phenomenon and impacts with emphasis on dust problems and present solutions in Khuzestan (Iran). Arhives of Hygiene Sciences. 2018;7(2):134–138. DOI: https://doi.org/10.29252/ArchHygSci.7.2.134.
- Akbari S. Dust storms, Sources in the Middle East and economic model for survey it s impacts. Australian Journal of Basic and Applied Sciences. 2011;5(12).
- Harrington AD, Schmidt MP, Szema AM, Galdanes K, Tsirka SE, Gordon T, et al. The role of Iraqi dust in inducing lung injury in United States soldiers – An interdisciplinary study. GeoHealth. 2017;1(5):237–246. DOI: https://doi.org/10.1002/2017gh000071.
- Sella SM, Netto ADP, Filho EVS, Araujo MT. Short-term and spatial variation of selected metals in the atmosphere of Niteroi City, Brazil. Microchemical Journal. 2004;78(1):85–90. DOI: https://doi.org/10.1016/j.microc.2004.03.015.
- Shah MH, Shaheen N. Annual and seasonal variations of trace metals in atmospheric suspended particulate matter in Islamabad, Pakistan. Water, Air, and Soil Pollution. 2008;190(1–4):13–25. DOI: https://doi.org/10.1007/s11270-007-9575-x.
- Wang C, Li W, Guo MX, Ji JF. Ecological risk assessment on heavy metals in soils: Use of soil diffuse reflectance mid-infrared Fourier-transform spectroscopy. Scientific Reports. 2017;7. DOI: https://doi.org/10.1038/srep40709.
- Li M, Gou HL, Al-Ogaidi I, Wu NQ. Nanostructured sensors for detection of heavy metals: A review. ACS Sustainable Chemistry and Engineering. 2013;1(7):713–723. DOI: https://doi.org/10.1021/sc400019a.
- Wang Q, Xie ZY, Li FB. Using ensemble models to identify and apportion heavy metal pollution sources in agricultural soils on a local scale. Environmental Pollution. 2015;206:227–235. DOI: https://doi.org/10.1016/j.envpol.2015.06.040.
- Duodu GO, Goonetilleke A, Ayoko GA. Comparison of pollution indices for the assessment of heavy metal in Brisbane River sediment. Environmental Pollution. 2016;219:1077–1091. DOI: https://doi.org/10.1016/j.envpol.2016.09.008.
- Ojedokun AT, Bello OS. Sequestering heavy metals from wastewater using cow dung. Water Resources and Industry. 2016;13:7–13. DOI: https://doi.org/10.1016/j.wri.2016.02.002.
- Motaghi M, Ziarati P. Adsorptive removal of cadmium and lead from oryza sativa rice by banana peel as bio-sorbent. Biomedical and Pharmacology Journal. 2016;9(2). DOI: https://doi.org/10.13005/bpj/998.
- Gholizadeh E, Ziarati P. Remediation of contaminated rice farmlands soil and Oryza sativa rice product by apple pomace as adsorbent. Biosciences, Biotechnology Research Asia. 2016;13(4):2245–2253. DOI: https://doi.org/10.13005/bbra/2390.
- Castro-Gonzalez NP, Calderon-Sanchez F, de Jesus JC, Moreno-Rojas R, Tamariz-Flores JV, Perez-Sato M, et al. Heavy metals in cow’s milk and cheese produced in areas irrigated with waste water in Puebla, Mexico. Food Additives and Contaminants: Part B – Surveillance. 2018;11(1):33–36. DOI: https://doi.org/10.1080/19393210.2017.1397060.
- Boloorani AD, Nabavi SO, Bahrami HA, Mirzapour F, Kavosi M, Abasi E, et al. Investigation of dust storms entering Western Iran using remotely sensed data and synoptic analysis. Journal of Environmental Health Science and Engineering. 2014;12. DOI: https://doi.org/10.1186/s40201-014-0124-4.
- Goudarzi G, Shirmardi M, Khodarahmi F, Hashemi-Shahraki A, Alavi N, Ankali KA, et al. Particulate matter and bacteria characteristics of the Middle East Dust (MED) storms over Ahvaz, Iran. Aerobiologia. 2014;30(4):345–356. DOI: https://doi.org/10.1007/s10453-014-9333-7.
- Burkart J, Steiner G, Reischl G, Moshammer H, Neuberger M, Hitzenberger R. Characterizing the performance of two optical particle counters (Grimm OPC1.108 and OPC1.109) under urban aerosol conditions. Journal of Aerosol Science. 2010;41(10):953–962. DOI: https://doi.org/10.1016/j.jaerosci.2010.07.007.
- Siddique N, Majid A, Chaudhry MM, Tufail M. Determination of heavy metals in air conditioner dust using FAAS and INAA. Journal of Radioanalytical and Nuclear Chemistry. 2012;292(1):219–227. DOI: https://doi.org/10.1007/s10967-011-1402-6.
- Simsek O, Gultekin R, Oksuz O, Kurultay S. The effect of environmental pollution on the heavy metal content of raw milk. Food. Nahrung. 2000;44(5):360–363. DOI: https://doi.org/10.1002/1521-3803(20001001)44:5<360::aidfood360>3.0.co;2-g.
- Sissakian VK, Al-Ansari N, Knutsson S. Sand and dust storm events in Iraq. Natural Science. 2013;5(10):1084–1094. DOI: https://doi.org/10.4236/ns.2013.510133.
- Akhzari D, Pessarakli M, Shayesteh K, Gonbad MB. Effect of source areas anthropogenic activities on dust storm occurrences in the western parts of Iran. Environmental Resources Research. 2014;2(2):124–132.
- Draxler RR, Gillette DA, Kirkpatrick JS, Heller J. Estimating PM10 air concentrations from dust storms in Iraq, Kuwait and Saudi Arabia. Atmospheric Environment. 2001;35(25):4315–4330. DOI: https://doi.org/10.1016/s1352-2310(01)00159-5.
- Shahsavani A, Naddafi K, Haghighifard NJ, Mesdaghinia A, Yunesian M, Nabizadeh R, et al. The evaluation of PM10, PM2.5, and PM1 concentrations during the Middle Eastern Dust (MED) events in Ahvaz, Iran, from april through september 2010. Journal of Arid Environments. 2012;77:72–83. DOI: https://doi.org/10.1016/j.jaridenv.2011.09.007.
- Teather K, Hogan N, Critchley K, Gibson M, Craig S, Hill J. Examining the links between air quality, climate change and respiratory health in Qatar. Avicenna. 2013;19. DOI: https://doi.org/10.5339/avi.2013.9.
- Grimm H, Eatough DJ. Aerosol measurement: the use of optical light scattering for the determination of particulate size distribution, and particulate mass, including the semi-volatile fraction. Journal of the Air and Waste Management Association. 2009;59(1):101–107. DOI: https://doi.org/10.3155/1047-3218.104.22.168.
- Jacobs DE, Clickner RP, Zhou JY, Viet SM, Marker DA, Rogers JW, et al. The prevalence of lead-based paint hazards in U.S. housing. Environmental Health Perspectives. 2002;110(10):A599–A606. DOI: https://doi.org/10.1289/ehp.021100599.
- Lu YY, Liang XQ, Niyungeko C, Zhou JJ, Xu JM, Tian GM. A review of the identification and detection of heavy metal ions in the environment by voltammetry. Talanta. 2018;178:324–338. DOI: https://doi.org/10.1016/j.talanta.2017.08.033.
- Wang ZQ, Wang H, Zhang ZH, Yang XJ, Liu G. Sensitive electrochemical determination of trace cadmium on a stannum film/poly(p-aminobenzene sulfonic acid)/electrochemically reduced graphene composite modified electrode. Electrochimica Acta. 2014;120:140–146. DOI: https://doi.org/10.1016/j.electacta.2013.12.068.
- Gharaibeh AA, El-Rjoob AWO, Harb MK. Determination of selected heavy metals in air samples from the northern part of Jordan. Environmental Monitoring and Assessment. 2010;160(1–4):425–429. DOI: https://doi.org/10.1007/s10661-008-0706-7.
- Al-Dabbas MA, Mahdi KH, Al-Khafaji R, Obayes KH, editors. Heavy metals characteristics of settled particles of streets dust from Diwaniyah City – Qadisiyah Governorate – Southern Iraq. IOP Conferences Series: Journal of Physics. 2018;1003. DOI: https://doi.org/10.1088/1742-6596/1003/1/012023.
- Heidari-Farsani M, Shirmardi M, Goudarz G, Alavi-Bakhtiarivand N, Ahmadi-Ankali K, Zallaghi E, et al. The evaluation of heavy metals concentration related to PM10 in ambient air of Ahvaz city, Iran. Journal of Advances in Environmental Health Research. 2014;1(2):120–128.
- Ziarati P, Alaedini S. The phytoremediation technique for cleaning up contaminated soil by Amaranthus sp. Journal of Environmental and Analytical Toxicology. 2014;4(2). DOI: https://doi.org/10.4172/2161-0525.1000208.
- Razafsha A, P. Ziarati P, Moslehishad M. Removal of heavy metals from Oryza sativa rice by sour lemon peel as biosorbent. Biomedical and Pharmacology Journal. 2016;9(2):543–553. DOI: https://doi.org/10.13005/bpj/971.
- Rezaei M, Dastjerdi HA, Jafari H, Farahi A, Shahabi A, Javdani H, et al. Assessment of dairy products consumed on the Arakmarket as determined by heavy metal residues. Health. 2014;6(5):323–327. DOI: https://doi.org/10.4236/health.2014.65047.
- Konuspayeva G, Jurjanz S, Loiseau G, Barci V, Akhmetsadykova S, Meldebekova AA, et al. Contamination of camel milk (heavy metals, organic pollutants and radionuclides) in Kazakhstan. Journal of Environmental Protection. 2011;2(1):90–96. DOI: https://doi.org/10.4236/jep.2011.21010.
- Evaluation of certain food additives and contaminants : forty-first report of the Joint FAO/WHO Expert Committee on Food Additives. World Health Organization techical report series. 1993;837:1–53.
- Alperkhdri AS, Abdullah MK, Khidhir ZK. Detection of some heavy metals residues in the local goat meat in kirkuk during the winter and summer seasons. Journal of Zankoy Sulaimani. 2018;(2):27–36. DOI: https://doi.org/10.17656/jzs.10649.
- Konuspayeva G, Napmuratova M, Meldebekova A, Faye B, Loiseau G. Variation factors of some minerals in camel milk. In: Faye B, Sinyavskiy Y, editors. Impact of Pollution on Animal Products. Dordrecht: Springer; 2008. pp. 125–132. DOI: https://doi.org/10.1007/978-1-4020-8359-4_13.
- Konuspayeva G, Faye B, Loiseau G, Diacono E, Akhmetsadykova S. Pollution of camel milk by heavy metals in Kazakhstan. The Open Environmental Pollution and Toxicology Journal. 2009;1:112–118.
- Awasthi V, Bahman S, Thakur LK, Singh SK, Dua A, Ganguly S. Contaminants in milk and impact of heating: An assessment study. Indian Journal of Piblic Health. 2012;56(1):95–99. DOI: https://doi.org/10.4103/0019-557X.96985.
- Malhat F, Hagag M, Saber A, Fayz AE. Contamination of cows milk by heavy metal in Egypt. Bulletin of Environmental Contamination and Toxicology. 2012;88(4):611–613. DOI: https://doi.org/10.1007/s00128-012-0550-x.
- Meshref AMS, Moselhy WA, Hassan NEY. Heavy metals and trace elements levels in milk and milk products. Journal of Food Measurement and Characterization. 2014;8(4):381–388. DOI: https://doi.org/10.1007/s11694-014-9203-6.
- Cai Q, Long ML, Zhu M, Zhou QZ, Zhang L, Liu J. Food chain transfer of cadmium and lead to cattle in a leadzinc smelter in Guizhou, China. Environmental Pollution. 2009;157(11):3078–3082. DOI: https://doi.org/10.1016/j.envpol.2009.05.048.
- Kabir A, Khan K, Khan IH, Jubair T, Jhahan E. A study of heavy metal presence in cow milk of different dairy farms near Karnafuli paper mills, Chittagong, Bangladesh. American Journal of Engineering Research. 2017;6(9):329–333.