Abstract

Introduction. Fish can sometimes be contaminated with several highly toxic substances at once, e.g. heavy metals, pesticides, and preservatives. In this regard, it is essential to determine the presence of these harmful chemicals in fish products. The research objective was to analyze the level of organochlorine pesticide residues and other toxic substances in Tenualosa ilisha L.
Study objects and methods. The study featured organochlorine pesticide residues and other toxic substances in raw and cooked samples of fresh and salted T. ilisha, which is a popular dish in Northeast India, especially in the state of Tripura. The analysis involved tests for formaldehyde, pesticides, and heavy metals. Formaldehyde content was estimated using high-performance liquid chromatography, pesticides content – by low-pressure gas chromatography/tandem mass spectrometry, and heavy metals – by inductively coupled plasma/mass spectrometry.
Results and discussion. The salted samples had a high content of formaldehyde, though it remained within the normal range. Both fresh and salted samples demonstrated high concentrations of heavy metals such as zinc, copper, and selenium. The salted sample appeared to have a high content of toxic organochlorine pesticide residues. Frying and boiling of fresh and salted fish decreased formaldehyde and organochlorine pesticide residue contents but did not reduce heavy metal content.
Conclusion. T. ilisha was found to be quite safe for human health.

Keywords

Tenualosa ilisha, formaldehyde, pesticides, heavy metals, cooking, chromatography, spectrometry

REFERENCES

1. Njoroge SW, Laposata M, Katrangi W, Seegmiller AC. DHA and EPA reverse cystic fibrosis-related FA abnormalities by suppressing FA desaturase expression and activity. Journal of Lipid Research. 2012;53(2):257–265. https://doi.org/10.1194/jlr.M018101.
2. Roos N, Islam M, Haraksingh Thilsted S. Small fish is an important dietary source of vitamin A and calcium in rural Bangladesh. International Journal of Food Sciences and Nutrition. 2003;54(5):329–339. https://doi.org/10.1080/09637480120092125.
3. Korelat M, Whitten T. Freshwater biodiversity in Asia: With special reference to fish. Washington: The World Bank, 1996. pp. 17–22.
4. Bhuiyan NI, Bhuiyan HR, Nath KK. Incidence of organochlorine insecticides (DDT and heptachlor) in Bangladeshi dry fish: Seasonal trends and species variability. African Journal of Environmental Science and Technology. 2009;3(11):405–411.
5. Siddique MAM, Aktar M. Detection of health hazard insecticide dichlorodiphenyltrichloroethane (DDT) in some common marine dry fish samples from Bangladesh. Health. 2012;4(4):185–189. https://doi.org/10.4236/health.2012.44027.
6. WHO global strategy for food safety: Safer food for better health. Geneva: World Health Organization; 2002. 23 p.
7. IARC monographs on the evaluation of carcinogenic risks to humans. Volume 88. Formaldehyde, 2-butoxyethanol and 1-tert-butoxypropan-2-ol. Lyon: International Agency for Research on Cancer; 2006. 278 p.
8. Weng X, Chon CH, Jiang H, Li D. Rapid detection of formaldehyde concentration in food on a polydimethylsiloxane (PDMS) microfluidic chip. Food Chemistry. 2009;114(3):1079–1082. https://doi.org/10.1016/j.foodchem.2008.10.027.
9. Consice International Chemical Assessment Document № 40. Formaldehyde. Geneva: World Health Organization; 2002. 75 p.
10. Additional foods ingredients. Jakarta; 1988.
11. Environmental Health Criteria 89. Formaldehyde. Geneva: World Health Organization; 1989.
12. Sabine M, Wendy G. Human health effects of heavy metals. Environmental Science and Technology Briefs for Citizens. 2009;(15).
13. Kafilzadeh F, Shiva AH, Malekpour R, Azad HN. Determination of organochlorine pesticide residues in water, sediments and fish from lake Parishan, Iran. World Journal of Fish and Marine Sciences. 2012;4(2):150–154.
14. Kumar N, Pathera AK, Saini P, Kumar M. Harmful effects of pesticides on human health. Annals of Agri Bio Research. 2012;17(2):125–127.
15. Ravindran J, Pankajshan M, Puthur S. Organochlorine pesticides, their toxic effects on living organisms and their fate in the environment. Interdisciplinary Toxicology. 2016;9(3–4):90–100. https://doi.org/10.1515/intox-2016-0012.
16. Gupta C. Invited review: Carbofuran toxicity ramesh. Journal of Toxicology and Environmental Health. 1994;43(4):383–418. https://doi.org/10.1080/15287399409531931.
17. Ahrens WH. Herbicide Handbook, Seventh Edition. Champaign: Weed Science Society of America; 1994. pp. 10–59.
18. Alam AKMN, Mohanty BP, Hoq ME, Thilshed S. Nutritional values,consumption and utilization of hilsa Tenualosa ilisha (Hamilton 1822). Collection of conference materials: Proc. Regional workshop on hilsa: Potential for aquaculture; 2012; Dhaka: WorldFish Regional Office; 2012.
19. Majumdar RK, Basu S, Anandan R. Biochemical and microbiological characteristics of salt fermented hilsa (Tenualosa ilisha). Fishery Technology. 2005;42(1):67–70.
20. Eriksson CE. Oxidation of lipids in food systems. In: Chan HW-S, editor. Autoxidation of unsaturated lipids. London: Academic Press. 1987. pp. 207–231.
21. Kimura M, Itokawa Y. Cooking losses of minerals in foods and its nutritional significance. Journal of Nutritional Science and Vitaminology. 1990;36:S25–S32. https://doi.org/10.3177/jnsv.36.4-supplementI_S25.
22. Salam KA, Hossain Motahar AKM, Khurshid Alam AHM, Pervin F, Absar N. A comparative analysis on physiochemical characteristic of oil extracted from six different parts of Hilsa fish (Hilsa ilisha). Pakistan Journal of Biological Sciences. 2005;8(6):810–815. https://doi.org/10.3923/pjbs.2005.810.815.
23. Claeys W, Vleminckx C, Dubois A, Huyghebaert A, Höfte M, Daenens P, et al. Formaldehyde in cultivated mushrooms: a negligible risk for the consumer. Food Additives and Contaminants – Part A Chemistry, Analysis, Control, Exposure and Risk Assessment. 2009;26(9):1265–1272. https://doi.org/10.1080/02652030903081929.
24. Anastassiades M, Lehotay SJ, Štajnbaher D, Schenck FJ. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solid-phase extraction” for the determination of pesticide residues in produce. Journal of AOAC International. 2003;86(2):412–431. https://doi.org/10.1093/jaoac/86.2.412.
25. Method 3051. Microwave assisted acid digestion of sediments, sludges, soils, and oils. 1994.
26. Leslie GB, Lunau FW. Indoor air pollution: problems and priorities. Cambridge: Cambridge University Press; 1994. 352 p.
27. Kibria G. Formalin and fish trade in Bangladesh – human and environmental risks. https://doi.org/10.13140/RG.2.1.4994.6087.
28. Tunhun D, Kanont S, Chaiyawat M, Raksakulthai N. Detection of illegal addition of formaldehyde to fresh fish. ASEAN Food Journal. 1996;11(2):74–77.
29. Abou-Arab AAK. Behavior of pesticides in tomatoes during commercial and home preparation. Food Chemistry. 1999;65(4):509–514. https://doi.org/10.1016/S0308-8146(98)00231-3.
30. Holand PT, Hamilton D, Ohlin B, Skidmore MW. Effects of storage and processing on pesticide residue in plant production. Pure and Applied Chemistry. 1994;66(2):335–356.
31. European Commission. Commission regulation (EC) no. 1881/2006 of 19 December 2006 setting maximum levels for certain contaminants in foodstuffs. Official Journal of the European Union. 2006:L364-5/L364-24.
32. Vincent JB. Recent advances in the nutritional biochemistry of trivalent chromium. Proceedings of the Nutrition Society. 2004;63(1):41–47. https://doi.org/10.1079/PNS2003315.
33. Nauen CE. Compilation of legal limits for hazardous substances in fish and fishery products. Rome: Food and Agriculture Organization; 1983. 102 p.