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Home >Foods and Raw Materials > Archive > Volume 5, Issue 1, 2017 > DETERMINATION OF THE INTENSITY OF BACTERIOCIN PRODUCTION BY STRAINS OF LACTIC ACID BACTERIA AND THEIR EFFECTIVENESS
ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

DETERMINATION OF THE INTENSITY OF BACTERIOCIN PRODUCTION BY STRAINS OF LACTIC ACID BACTERIA AND THEIR EFFECTIVENESS

M. I. Zimina a
,
A. F. Gazieva a
,
J. Pozo-Dengra b
,
S. Yu. Noskova a
,
A. Yu. Prosekov c
Affiliation
a Kemerovo Institute of Food Science and Technology (University), Kemerovo, Russia
b Biorizon Biotech, Science and Technology Park of Almería (PITA), Almeria, Spain
c Kemerovo State University, Kemerovo, Russia
Copyright ©M. et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
DOI: http://doi.org/10.21179/2308-4057-2017-1-108-117
Abstract
The study of bacteriocins is one of the most relevant areas of research. This is due to the increase in the number of pathogenic microorganisms resistant to antibiotics. The bacteriocins of lactic acid bacteria have the most common application because of their safety in use. To assess the antimicrobial potential of bacteriocins of lactic acid bacteria and the possibilities for their further use, this study deals with the intensity of bacteriocin production by strains of lactic acid bacteria, the minimal inhibitory concentration of bacteriocins produced by these strains with respect to E. coli B-6954, as well as the antimicrobial activity of the produced bacteriocins with respect to some strains of pathogenic microorganisms. This work was carried out on the basis of the Kemerovo Technological Institute of Food Industry (Russia). The study objects were strains of microorganisms Lactobacillus delbrueckii B2455, Lactobacillus paracasei B2430, Lactobacillus plantarum B884. Biomass concentration was determined by spectrophotometric method. Study of antimicrobial activity in relation to a number of pathogenic strains was carried out in a culture fluid by optical density, and also by a disk-diffusion test. As a result, the maximum productivity value, characterizing the intensity of bacteriocin production, was observed for strain B884. However, the quality of bacteriocin (antimicrobial activity relative to the E. coli strain) with a lower productivity is higher for strain B2430. The minimum inhibitory concentration for the strains studied was: B2430 - 1.0 106 CFU/ml, B884 -1.0 108 CFU/ml, B2455 - 1.0 107 CFU/ml. The best indicator of the minimum inhibitory concentration was observed for strain B2430. Thus the strain B2430 had the greatest antimicrobial activity, it had an inhibitory effect on 12 test cultures: Escherichia coli , Alcaligenes faecalis , Candida albicans , Pseudomonas libanensis , Staphylococcus warneri, Erwinia aphidicola, Microbacterium foliorum, Bacillus licheniformis , Serratia plymuthica, Erwinia aphidicola, Bacillus endophyticus, Leuconostoc mesenteroides . Thus, among the test Lactobacillus strains, the strain of Lactobacillus paracasei B2430 is promising for further study.
Keywords
Lactic acid bacteria, bacteriocins, antimicrobial activity, biomass concentration, minimum inhibitory concentration
REFERENCES
  1. Zimina, M.I., Prosekov, A.J., Babich, O.O., Sukhih, S.A. Identification and studying of the biochemical properties of lactobacillus strains. Life Science Journal, 2014, vol. 11, iss. 11, pp. 338-341.
  2. Astakhova L., Babich O., Prosekov A., et al. Short chain fatty acids (SCFA) reprogram gene expression in human malignant epithelial and lymphoid cells. PLoS ONE, 2016, vol. 11, no. 7, e0154102. DOI: 10.1371/journal.pone.0154102.
  3. Shishin M.V. and Prosekov A.Yu. Investigation of morphological and antimicrobial properties of intestinal tract microorganisms. Food Processing: Techniques and Technology, 2015, vol. 39, no. 4, pp. 131-137. (In Russian).
  4. Walker E.S. Genetic trends in a population evolving antibiotic resistance. Evolution, 2001, vol.55, pp. 1-20.
  5. Dyshlyuk L., Babich O., Belova D., and Prosekov A. Comparative analysis of physical and chemical properties of biodegradable edible films of various compositions. Journal of food process engineering, 2017, vol. 40, n/a, e12331. DOI: 10.1111/jfpe.12331.
  6. Zimina M.I., Sukhih S.A., Babich O.O., Noskova S.Yu., Abrashina A.A., and Prosekov A.Yu. Investigating antibiotic activity of the genus Bacillus strains and properties of their bacteriocins in order to develop next-generation pharmaceuticals. Foods and Raw Materials, 2016, vol. 4, no.2, pp. 95-100. DOI: 10.21179/2308-4057-2016-2-92-100.
  7. Piskaeva A.I., Sidorin Yu.Yu., and Prosekov A.Yu. Comparative Analysis of the Activity of Silver Nanoparticles against Native Microflora from Poultry Processing Plants Wastes. Nano Hybrids and Composites, 2017, vol. 13, pp. 176-183. DOI: 10.4028/www.scientific.net/NHC.13.176.
  8. Bali V., Panesar P.S., Bera M.B., and Kennedy J.F. Bacteriocins: Recent Trends and Potential Applications. Critical Reviews in Food Science and Nutrition, 2016, vol. 56, no. 5, pp. 34-817. DOI: 10.1080/10408398.2012.729231.
  9. Cleveland J., Montville T.J., Nes I.F., and Chikindas M.L. Bacteriocins: safe, natural antimicrobials for food preservation. International Journal of Food Microbiology, 2001, vol. 71, no. 1, pp. 71-120. DOI: 10.1016/S0168-1605(01)00560-8.
  10. Surendran Nair N., Amalaradjou M.A., and Venkitanarayanan K. Chapter One - Antivirulence Properties of Probiotics in Combating Microbial Pathogenesis. Advances in Applied Microbiology, 2017, vol. 98, pp. 1-29. DOI: 10.1016/bs.aambs.2016.12.001.
  11. Lohans C.T. and Vederas J.C. Development of Class IIa Bacteriocins as Therapeutic Agents. International Journal of Microbiology, 2012, pp. 386-410. DOI: http://dx.doi.org/10.1155/2012/386410.
  12. Perez R.H., Perez M.T.M., and Elegado F.B. Bacteriocins from lactic acid bacteria: A review of biosynthesis, mode of action, fermentative production, uses and prospects. International Journal of Philippine Science and Technology, 2015, vol. 8, no. 2, pp. 61-67.
  13. Prosekov A.Yu. and Ivanova S.A. Providing food security in the existing tendencies of population growth and political and economic instability in the world. Foods and Raw Materials, 2016, vol. 4, no. 2, pp. 201-211. DOI: 10.21179/2308-4057-2016-2-201-211.
  14. Gálvez A., Abriouel H., López R.L., and Omar N.B. Bacteriocin based strategies for food bio- preservation. International Journal of Food Microbiology. 2007, vol. 120, no. 1-2, pp. 51-70. DOI: 10.1016/j.ijfoodmicro.2007.06.001.
  15. Cotter P.D., Hill C., and Ross R.P. Bacteriocins: developing innate immunity for food. Nature reviews. Microbiology, 2005, vol. 3, pp. 777-788. DOI: 10.1038/nrmicro1273.
  16. Pilasombut K., Rumjuankiat K., Ngamyeesoon N., and Duy le N.D. In vitro Characterization of Bacteriocin Produced by Lactic Acid Bacteria Isolated from Nem Chua, a Traditional Vietnamese Fermented Pork. Korean Journal for Food Science of Animal Resources, 2015, vol. 35, no. 4, pp. 8-473. DOI: 10.5851/kosfa.2015.35.4.473.
How to quote?
Zimina M.I., Gazieva A.F., Pozo-Dengra J., Noskova S.Yu., and Prosekov A.Yu. Determination of the intensity of bacteriocin production by strains of lactic acid bacteria and their effectiveness. Foods and Raw Materials, 2017, vol. 5, no. 1, pp. 108–117. DOI: 10.21179/2308-4057-2017-1-108-117.
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