Abstract

The present article considers the increasing popularity of beverages containing ethyl alcohol and fermentation products with the population of Russia. Statistical data show that alcoholic beverages with ethanol concentration exceeding 40 vol. % account for the largest share of the market. The development of a procedure for the production of alcohol oxidase from the yeast Candida boidinii is reported; the enzyme is intended for use in the manufacturing of functional foods for withdrawal syndrome alleviation. A procedure for the disruption of cell walls of the yeast Candida boidinii in a planetary ball mill PM 400 and methods for the removal of ballast substances reducing the catalytic efficiency and the specific activity of the enzyme preparation are presented.

Keywords

withdrawal syndrome, foods, beverages, alcohol oxidase, catalase, technology, purification

REFERENCES

1. Report on alcohol and health 2010. World Health Organization. URL: http://www.euro.who.int/__data/assets/ pdf_file/0004/128065/e94533.pdf
2. Sotsial´noe polozhenie i uroven´ zhizni naseleniya Rossii: Stat. Sb. (Social Situation and Living Standards of the Popualtion of Russia: Stat. Comp.) (Rosstat, Moscow, 1997–2010).
3. Krevyakina, E.V., Medvedev: «V bednoy strane p´yanstvo ne pobedit´» (Medvedev: “A poor country cannot defeat alcoholism”), Komsomol’skaya Pravda, August 09, 2009.
4. Pleteneva, T. V., Toksikologicheskaya khimiya (Toxicological Chemistry) (GEOTAR Media, Moscow, 2005).
5. Pavlishko, H., Gayda, H., and Gonchar, M., Alcohol oxidase and its bioanalytical application, Vysnyk of L´viv Univ. Biology series, 2004. № 35. P. 3.
6. Rumyantsev, A.P., Tiunov, L.V., and Ostroumova, I.A., Metabolizm organicheskikh soedinenii opiinogo ryada (Metabolism of a range of organic opioid compounds), Itogi nauki i tekhniki (Achievements in Science and Technology) (VINITI, Moscow, 1981). V. 12. P. 65.
7. Gonchar, M., Metabolically engineered methylotrophic yeast cells and enzymes as sensor biorecognition elements, FEMS Yeast Research, 2002. V. 2. P. 307.
8. Ashin, V.V. and Trotsenko, Yu.A., Alcohol oxidase of the methylotrophic yeasts: new findings, J. Mol. Catal. B: Enzym., 2000. V. 10. № 1-3. P. 295.
9. Yeast Biotechnology: Diversity and Applications, ed. by Satyanarayana, T. and Kunze, G. (2009).
10. Prosekov, A.Yu, Babich, O.O., and Sukhikh, S.A., Sovremennye metody issledovaniya syr´ya i biotekhnologicheskoi produktsii: Laboratornyi praktikum (Modern Methods of Analysis of Raw Materials and Biotechnology Products: A Laboratory Guide) (Kemerovo Technological Institute of Food Industry, Kemerovo, 2012).
11. Shugalei, I.V., Garabadzhiu, A.V., and Tselinskii, I.V., Khimiya belka: uchebnoe posobie (Study Materials in Protein Chemistry) (Prospekt Nauki, St. Petersburg, 2011).
12. Scopes R, Metody ochistki belkov (Protein Purification Techniques), Moscow, Mir, 1985.
13. Osterman, L.A., Khromatografiya belkov i nukleinovykh kislot (Chromatography of Proteins and Nucleic Acids) (Nauka, Moscow, 1985).
14. Bystrykh, L.V., Dvorokova, J., and Volfova, O. Alcohol oxidase of methylotrophic thermo- and acidotolerant yeast Hansenula sp., Folia Microbol., 1989. V. 34. № 1. P. 233.
15. Plakunov, V.K. Osnovy enzimologii (Fundamentals of Enzymology) (Logos, Moscow, 2001).
16. Mizgunova, U.M., Shekhovtsova, T.N., and Dolmanova, I.F., Fermentnye metody opredeleniya alifaticheskikh spirtov (Enzymatic methods for the determination of aliphatic alcohols), J. Anal. Chem. (Moscow), 1998. V. 53. № 10. P. 1014.
17. Azevedo, A.M., Prazeres, D.M., Cabral, J.M., and Fonseca, L.P. Ethanol biosensors based on alcohol oxidase, Biosens Bioelectron., 2006. V. 21. № 12. P. 2306.