«Department of scientific and publishing activities»
Ru En
Open access
Subscription/purchasing
Submit manuscript
Home >Foods and Raw Materials > Archive > Volume 6, Issue 2, 2018 > Prospects for DNA authentication in wine production monitoring
ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Prospects for DNA authentication in wine production monitoring

Lev A. Oganesyants a
,
Vafin R. b
,
Aram G. Galstyan b
,
Semipyatniy V. b
,
Khurshudyan Sergey. c
,
Anastasia E. Ryabova b
Affiliation
a All-Russian Research Institute of Brewing, Non-Alcoholic and Wine Industry
b All-Russian Research Institute of Brewing, Non-Alcoholic and Wine Industry – branch of FGBNU «FSC foods systems by V.M. Gorbatov» RAS
c VNIIPBiVP
Copyright ©Oganesyants et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (http://creativecommons.org/licenses/by/4.0/), allowing third parties to copy and redistribute the material in any medium or format and to remix, transform, and build upon the material for any purpose, even commercially, provided the original work is properly cited and states its license.
DOI: http://doi.org/10.21603/2308-4057-2018-2-438-448
Abstract
Wines DNA authentication is a technological process of their authenticity verification by genetic identification of the main plant ingredient by means of molecular genetic analysis of the residual amounts of Vitis vinifera L nucleic acids extracted from end product cellular debris. The main aim of the research was the analysis of scientific and methodological approaches to the extraction of residual amounts of nucleic acids in wine raw materials and DNA authentication of wines for their subsequent application in solving the problem of determining wine products authenticity and place of origin. The prior art includes various approaches to the extraction of Vitis vinifera L. nucleic acids among which the three methods by Savazzini & Martinelli, Pereira and Bigliazzi can be named basically. Analysis of the effectiveness of different methods of DNA extraction from wines indicates the superiority of the Pereira method over other traditional methods of extraction in terms of DNA yield and quality. Besides, the nucleic acid extracted from wines is characterized as residual since its concentration is significantly reduced in a multi-stage wine production process. The yield of extracted nucleic acid also decreases as the wine ages. The use of microsatellite DNA loci designed for grapes genetic identification is one of the approaches applicable for wine DNA authentication.
Keywords
Wine, grapes, variety, Vitis vinifera L, DNA, authentication, identification, marker, SSR, SNP, PCR, HRM analysis
REFERENCES
  1. Oganesyants L.A. Falʹsifikaty vinodelʹcheskoy produktsii: metody vyyavleniya [Counterfeit wine products: detection methods]. Production Quality Control, 2017, no. 7, pp. 8–11. (In Russ.).
  2. Parkhomenko A.I. Identification and detection of wine falsifications for customs purposes. Obrazovanie i nauka bez granits: sotsialʹno-gumanitarnye nauki [Education and Science Without Borders: Social and Human Sciences], 2016, no. 3, pp. 298–301. (In Russ.).
  3. Ebeler S.E., Takeoka G.R., and Winterhalter P. Progress in Authentication of Food and Wine. ACS Symposium Series, 2011, vol. 1081. DOI: https://doi.org/10.1021/bk-2011-1081.
  4. Montet D. and Ray R.C. Food Traceability and Authenticity: Analytical Techniques. Boca Raton, Florida: CRC Press, 2017, 354 p. DOI: https://doi.org/10.1201/9781351228435.
  5. Anikina N.S., Gnilomedova N.V., Agafonova N.M., and Riabinina O.V. Peculiarities of regulatory requirements for the control of quality and safety of wines. Magarach. Viticulture and Enology, 2016, no. 3, pp. 37–43. (In Russ.).
  6. Yakuba Yu.F. and Temerdashev Z.A. Chromatography methods in the analysis and identification of grape wines. Analytics and Control, 2015, vol. 19, no. 4, pp. 288–301. DOI: https://doi.org/10.15826/analitika.2015.19.4.013. (In Russ.).
  7. Oganesyants L.A., Panasyuk A.L., Kuzmina E.I., and Kharlamova L.N. Determination of the carbon isotope 13C/12C in ethanol of fruit wines in order to define identification characteristics. Foods and Raw Materials, 2016, vol. 4, no. 1, pp. 141–147. DOI: https://doi.org/10.21179/2308-4057-2016-1-141-147.
  8. Christoph N., Hermann A., and Wachter H. 25 Years authentication of wine with stable isotope analysis in the European Union – Review and outlook. BIO Web of Conferences, 2015, no. 5. DOI: https://doi.org/10.1051/bioconf/20150502020.
  9. Oganesyants L.A., Khurshudyan S.A., and Galstyan A.G. Monitoring kachestva pishchevykh produktov – bazovyy ehlement strategii [Food quality monitoring as the basic strategic element]. Production Quality Control, 2018, no. 4, pp. 56–59. (In Russ.).
  10. İşçi B., Yildirim H.K., and Altindişli A. A Review of the Authentication of Wine Origin by Molecular Markers. Journal of the Institute of Brewing, 2009, vol. 115, no. 3, pp. 259–264. DOI: https://doi.org/10.1002/j.2050-0416.2009.tb00378.x.
  11. Catalano V., Moreno-Sanz P., Lorenzi S., and Grando M.S. Experimental Review of DNA-Based Methods for Wine Traceability and Development of a Single-Nucleotide Polymorphism (SNP) Genotyping Assay for Quantitative Varietal Authentication. Journal of Agricultural and Food Chemistry, 2016, vol. 64, no. 37, pp. 6969–6984. DOI: https://doi.org/10.1021/acs.jafc.6b02560.
  12. Pereira L., Gomes S., Castro C., et al. High Resolution Melting (HRM) applied to wine authenticity. Food Chemistry, 2017, vol. 216, pp. 80–86. DOI: https://doi.org/10.1016/j.foodchem.2016.07.185.
  13. Baleiras-Couto M.M. and Eiras-Dias J.E. Detection and identification of grape varieties in must and wine using nuclear and chloroplast microsatellite markers. Analytica Chimica Acta, 2006, vol. 563, no. 1–2, pp. 283–291. DOI: https://doi.org/10.1016/j.aca.2005.09.076.
  14. Savazzini F. and Martinelli L. DNA analysis in wines: Development of methods for enhanced extraction and real-time polymerase chain reaction quantification. Analytica Chimica Acta, 2006, vol. 536, no. 1–2, pp. 274–282. DOI: https://doi.org/10.1016/j.aca.2005.10.078.
  15. Nakamura S., Haraguchi K., Mitani N., and Ohtsubo K. Novel Preparation Method of Template DNAs from Wine for PCR to Differentiate Grape (Vitis vinifera L.) Cultivar. Journal of Agricultural and Food Chemistry, 2007, vol. 55, no. 25, pp. 10388–10395. DOI: https://doi.org/10.1021/jf072407u.
  16. Pereira L., Guedes-Pinto H., and Martins-Lopes P. An Enhanced Method for Vitis vinifera L. DNA Extraction from Wines. American Journal of Enology and Viticulture, 2011, vol. 62, no. 4, pp. 547–552. DOI: https://doi.org/10.5344/ajev.2011.10022.
  17. Bigliazzi J., Scali M., Paolucci E., Cresti M., and Vignani R. DNA Extracted with Optimized Protocols Can Be Genotyped to Reconstruct the Varietal Composition of Monovarietal Wines. American Journal of Enology and Viticulture, 2012, vol. 63, no. 4, pp. 568–573. DOI: https://doi.org/10.5344/ajev.2012.12014.
  18. Rodríguez-Plaza P., González R., Moreno-Arribas M.V., et al. Combining microsatellite markers and capillary gel electrophoresis with laser-induced fluorescence to identify the grape (Vitis vinifera) variety of musts. European Food Research and Technology, 2006, vol. 223, no. 5, pp. 625–631. DOI: https://doi.org/10.1007/s00217-005-0244-2.
  19. Drábek J., Stávek J., Jalvková M., Jurcek T., and Frébort I. Quantification of DNA during winemaking by fluorimetry and Vitis vinifera L.-specific quantitative PCR. European Food Research and Technology, 2008, vol. 226, no. 3, pp. 491–497. DOI: https://doi.org/10.1007/s00217-007-0561-8.
  20. Garcia-Beneytez E., Maria V.M., Joaquin B., Maria C.P., and Javier I. Application of a DNA Analysis Method for the Cultivar Identification of Grape Musts and Experimental and Commercial Wines of Vitis vinifera L. Using Microsatellite Markers. Journal of Agricultural and Food Chemistry, 2002, vol. 50, no. 21, pp. 6090–6096. DOI: https://doi.org/10.1021/jf0202077.
  21. Siret R., Gigaud O., Rosec J.P., and This P. Analysis of Grape Vitis vinifera L. DNA in Must Mixtures and Experimental Mixed Wines Using Microsatellite Markers. Journal of Agricultural and Food Chemistry, 2002, vol. 50, no. 13, pp. 3822–3827. DOI: https://doi.org/10.1021/jf011462e.
  22. Hârta M.H., Pamfil D., Pop R., and Vicaş S. DNA Fingerprinting Used for Testing Some Romanian Wine Varieties. Bulletin UASVM Horticulture, 2011, vol. 68, no. 1, pp. 143–148. DOI: https://doi.org/10.15835/buasvmcn-hort:7041.
  23. Thomas M.R. and Scott N.S. Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence-tagged sites (STSs). Theoretical and Applied Genetics, 1993, vol. 86, no. 8, pp. 985–990. DOI: https://doi.org/10.1007/BF00211051.
  24. Bowers J.E., Dangl G.S., Vignani R., and Meredith C.P. Isolation and characterization of new polymorphic simple sequence repeat loci in grape (Vitis vinifera L.). Genome, 1996, vol. 39, no. 4, pp. 628–633. DOI: https://doi.org/10.1139/g96-080.
  25. Sefc K.M., Regner F., Turetschek E., Glössl J., and Steinkellner H. Identification of microsatellite sequences in Vitis riparia and their applicability for genotyping of different Vitis species. Genome, 1999, vol. 42, no. 3, pp. 367–373.
  26. Maul E., Töpfer R., Carka F., et al. Identification and characterization of grapevine genetic resources maintained in Eastern European Collections. Vitis - Journal of Grapevine Research, 2015, vol. 54, pp. 5–12.
  27. This P., Jung A., Boccacci P., et al. Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics, 2004, vol. 109, no. 7, pp. 1448–1458. DOI: https://doi.org/10.1007/s00122-004-1760-3.
  28. Siret R., Boursiquot J.M., Merle M.H., Cabanis J.C., and This P. Toward the Authentication of Varietal Wines by the Analysis of Grape (Vitis vinifera L.) Residual DNA in Must and Wine Using Microsatellite Markers. Journal of Agricultural and Food Chemistry, 2000, vol. 48, no. 10, pp. 5035–5040. DOI: https://doi.org/10.1021/jf991168a.
  29. Boccacci P., Akkak A., Marinoni D.T., Gerbi V., and Schneider A. Genetic traceability of Asti Spumante and Moscato d’Asti musts and wines using nuclear and chloroplast microsatellite markers. European Food Research and Technology, 2012, vol. 235, no. 3, pp. 439–446. DOI: https://doi.org/10.1007/s00217-012-1770-3.
  30. Pereira L., Martins-Lopes P., Batista C., et al. Molecular Markers for Assessing Must Varietal Origin. Food Analytical Methods, 2012, vol. 5, no. 6, pp. 1252–1259. DOI: https://doi.org/10.1007/s12161-012-9369-7.
  31. Sefc K.M., Lefort F., Grando M.S., et al. Microsatellite markers for grapevine: A state of the art. In: Roubelakis-Angelakis K.A. (ed) Molecular Biology and Biotechnology of the Grapevine. Dordrecht: Springer Netherlands Publ., 2001, pp. 433–463. DOI: https://doi.org/10.1007/978-94-017-2308-4.
  32. Pellerone F.I., Edwards K.J., and Thomas M.R. Grapevine microsatellite repeats: Isolation, characterization and use for genotyping of grape germplasm from Southern Italy. Vitis, 2001, vol. 40, no. 4, pp. 179–186.
  33. Hârta M. and Pamfil D. Molecular Characterisation of Romanian Grapevine Cultivars Using Nuclear Microsatellite Markers. Bulletin UASVM Horticulture, 2013, vol. 70, no. 1, pp. 131–136.
  34. Ghetea L.G., Motoc R.M., Popescu C.F., Barbacar N., et al. Genetic profiling of nine grapevine cultivars from Romania, based on SSR markers. Romanian Biotechnological Letters, 2010, vol. 15, no. 1, pp. 116–124.
  35. Bowers J.E., Dangl G.S., and Meredith C.P. Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture, 1999, vol. 50, no. 3, pp. 243–246.
  36. Dokupilováa I., Šturdíka E., and Mihálik D. Characterization of vine varieties by SSR markers. Acta Chimica Slovaca, 2013, vol. 6, no. 2, pp. 227–234. DOI: https://doi.org/10.2478/acs-2013-0035.
  37. Sant'Ana G.C., Ferreira J.L., Rocha H.S., et al. Comparison of a retrotransposon-based marker with microsatellite markers for discriminating accessions of Vitis vinifera. Genetics and Molecular Research, 2012, vol. 11, no. 2, pp. 1507–1525. DOI: https://doi.org/10.4238/2012.May.21.8.
  38. Carimi F., Mercati F., De Michele R., et al. Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d’Avola’ as revealed by microsatellite markers. Genetic Resources and Crop Evolution, 2011, vol. 58, no. 7, pp. 967–975. DOI: https://doi.org/10.1007/s10722-011-9731-4.
  39. Guo D.-L., Yu Y.-H., Xi F.-F., Shi Y.-Y., and Zhang G.-H. Histological and Molecular Characterization of Grape Early Ripening Bud Mutant. International Journal of Genomics, 2016, 7 p. DOI: https://doi.org/10.1155/2016/5620106.
  40. Aversano R., Basile B., Buonincontri M.P., et al. Dating the beginning of the Roman viticultural model in the Western Mediterranean: The case study of Chianti (Central Italy). PLoS One, 2017, vol. 12, no. 11. DOI: https://doi.org/10.1371/journal.pone.0186298.
  41. Lukyanov A.A., Bolshakov V.A., Ilnitskaya E.T. Creation of database and DNA-sertification of varieties of anapic ampelographic collection. Fruit growing and viticulture of South Russia, 2018, vol. 51, no. 3, pp. 49–58. DOI: https://doi.org/10.30679 / 2219-5335-2018-3-51-49-58. (In Russ.).
  42. Scali M., Elisa P., Jacopo B., Mauro C., and Vignani R. Vineyards genetic monitoring and Vernaccia di San Gimignano wine molecular fingerprinting. Advances in Bioscience and Biotechnology, 2014, vol. 5, no. 2, pp. 142–154. DOI: https://doi.org/10.4236/abb.2014.52018.
  43. Chung S.-M. and Staub J.E. The development and evaluation of consensus chloroplast primer pairs that possess highly variable sequence regions in a diverse array of plant taxa. Theoretical and Applied Genetics, 2003, vol. 107, no. 4, pp. 757–767. DOI: https://doi.org/10.1007/s00122-003-1311-3.
  44. Weising K. and Gardner R.C. A set of conserved PCR primers for the analysis of simple sequence repeat polymorphisms in chloroplast genomes of dicotyledonous angiosperms. Genome, 1999, vol. 42, no. 1, pp. 9–19. DOI: https://doi.org/10.1139/g98-104.
  45. Arroyo-Garcı´a R., Lefort F., de Andre´s M.T., et al. Chloroplast microsatellite polymorphisms in Vitis species. Genome, 2002, vol. 45, no. 6, pp. 1142–1149. DOI: https://doi.org/10.1139/g02-087.
  46. Ebert D. and Peakall R. Chloroplast simple sequence repeats (cpSSRs): technical resources and recommendations for expanding cpSSR discovery and applications to a wide array of plant species. Molecular Ecology Resources, 2009, vol. 9, no. 3, pp. 673–690. DOI: https://doi.org/10.1111/j.1755-0998.2008.02319.x.
  47. Bryan G.J., McNicoll J., Ramsay G., and Meyer R.C. Polymorphic simple sequence repeat markers in chloroplast genomes of Solanaceous plants. Theoretical and Applied Genetics, 1999, vol. 99, pp. 859–867. DOI: https://doi.org/10.1007/s001220051306.
  48. Arroyo-García R., Ruiz-García L., Bolling L., et al. Multiple origins of cultivated grapevine (Vitis vinifera L. ssp. sativa) based on chloroplast DNA polymorphisms. Molecular Ecology, 2006, vol. 15, no. 12, pp. 3707–3714. DOI: https://doi.org/10.1111/j.1365-294X.2006.03049.x.
  49. Weising K., Winter P., Hüttel B., and Kahl G. Microsatellite Markers for Molecular Breeding. Journal of Crop Production, 1997, vol. 1, no. 1, pp. 113–143. DOI: https://doi.org/10.1300/J144v01n01_06.
  50. Pe´ros J.-P., Berger G., Portemont A., Boursiquot J.-M., and Lacombe T. Genetic variation and biogeography of the disjunct Vitis subg. Vitis (Vitaceae). Journal of Biogeography, 2011, vol. 38, no. 3, pp. 471–486. DOI: https://doi.org/10.1111/j.1365-2699.2010.02410.x.
  51. Nishikawa T., Vaughan D.A., and Kadowaki K. Phylogenetic analysis of Oryza species, based on simple sequence repeats and their flanking nucleotide sequences from the mitochondrial and chloroplast genomes. Theoretical and Applied Genetics, 2005, vol. 110, no. 4, pp. 696–705. DOI: https://doi.org/10.1007/s00122-004-1895-2.
  52. Bergman, C.J., Bhattacharya, K.R., Ohtsubo, K. Rice end-use quality analysis. In: Champagne E.T. (ed) Rice: Chemistry and Technology. 3 ed. St. Paul, USA: American Association of Cereal Chemists Publ., 2004. pp. 415–472.
  53. Ohtsubo K. and Nakamura S. Cultivar Identification of Rice (Oryza sativa L.) by Polymerase Chain Reaction Method and Its Application to Processed Rice Products. Journal of Agricultural and Food Chemistry, 2007, vol. 55, no. 4, pp. 1501–1509. DOI: https://doi.org/10.1021/jf062737z.
  54. Ohtsubo K., Nakamura S., Yoza K., and Shishido K. Identification of glutinous rice cultivars using rice cake as samples by the PCR method. Journal of the Japanese Society for Food Science and Technology, 2001, vol. 48, no. 4, pp. 306–310.
  55. Lijavetzky D., Cabezas J.A., Ibáñez A., Rodríguez V., and Martínez-Zapater J.M. High throughput SNP discovery and genotyping in grapevine (Vitis vinifera L.) by combining a re-sequencing approach and SNPlex technology. BMC Genomics, 2007, vol. 8, pp. 424. DOI: https://doi.org/10.1186/1471-2164-8-424.
  56. Cabezas J.A., Ibáñez J., Lijavetzky D., et al. A 48 SNP set for grapevine cultivar identification. BMC Plant Biology, 2011, vol. 11, pp. 153. DOI: https://doi.org/10.1186/1471-2229-11-153.
  57. Pereira L. and Martins-Lopes P. Vitis vinifera L. Single-Nucleotide Polymorphism Detection with High-Resolution Melting Analysis Based on the UDP-Glucose: Flavonoid 3-O-Glucosyltransferase Gene. Journal of Agricultural and Food Chemistry, 2015, vol. 63, no. 41, pp. 9165–9174. DOI: https://doi.org/10.1021/acs.jafc.5b03463.
  58. Gomes S., Castro C., Barrias S., et al. Alternative SNP detection platforms, HRM and biosensors, for varietal identification in Vitis vinifera L. using F3H and LDOX genes. Scientific Reports, 2018, vol. 8, no. 1, pp. 5850. DOI: https://doi.org/10.1038/s41598-018-24158-9.
  59. Druml B. and Cichna-Markl M. High resolution melting (HRM) analysis of DNA – Its role and potential in food analysis. Food Chemistry, 2014, vol. 158, pp. 245–254. DOI: https://doi.org/10.1016/j.foodchem.2014.02.111.
  60. Petrov A.N., Khanferyan R.A., and Galstyan A.G. Current aspects of counteraction of foodstuff's falsification. Problems of Nutrition, 2016, vol. 85, no. 5, pp. 86–92. (In Russ.).
How to quote?
About journal

Contents
Abstract
Keywords
References
Foods and Raw Materials uses cookies. By continuing to use the portal, you agree to the storage and use of cookies by the portal and partner sites on your device.
Manage files