Affiliation
a National Research Centre, Cairo
b National Resaerch Centre, Cairo
c National Research Centre
Copyright ©Mohamed et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0. (
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Received 08 August, 2023 |
Accepted in revised form 07 November, 2023 |
Published 23 October, 2024
Abstract
This study explores the potential of utilizing quinoa protein as an egg substitute in bakery products for customers with health, culture/religion, or dietary restrictions.
Quinoa protein was prepared from quinoa seed by alkaline solubilization followed by isoelectric precipitation and drying. Four different formulations of egg-free cakes were prepared by incorporating quinoa protein in egg equivalents of 50 g (Formulation 1), 75 g (Formulation 2), 100 g (Formulation 3), and 150 g (Formulation 4). The research involved Fourier-transform infrared spectroscopy and revealed such functional properties as proximate composition, physical properties, color, texture, microstructure, and sensory characteristics for the batters and the cakes.
The incorporation of different quinoa protein concentrations significantly (p < 0.05) affected all the functional properties of the batters and the cakes. Such variables as crude protein and ash increased while moisture and fat contents decreased. The baking loss went down as the share of quinoa protein went up. The structural analysis showed an increase in gumminess and chewiness accompanied by a decrease in cohesiveness and elasticity. The analysis also revealed hardness and non-uniform changes. The lightness (L*) and yellowness (b*) of the cake surface and crumb decreased while the redness (a*) increased.
The cakes prepared according to Formulation 4 with the greatest share of quinoa protein had a high nutritional value with reasonable concentrations of essential amino acids in general and a high level of lysine in particular. The same sample also received the highest score for overall sensory properties. The sensory assessment proved that quinoa protein could meet consumer expectations of egg-free cakes.
Keywords
Quinoa protein isolate,
functional cake,
egg-free products,
microstructure,
functional and physicochemical attributes,
amino acid composition
REFERENCES
- Bazile D, Baudon F. The dynamics of the global expansion of quinoa growing in view of its high biodiversity. In: Bazile D, Bertero D, Nieto C, editors. State of the art report of quinoa in the world in 2013. Rome: FAO & CIRAD; 2013. pp. 42–55.
- Agarwal A, Rizwana, Tripathi AD, Kumar T, Sharma KP, Patel SKS. Nutritional and functional new perspectives and potential health benefits of quinoa and chia seeds. Antioxidants. 2023;12(7):1413. https://doi.org/10.3390/antiox12071413
- Gojković Cvjetković VS, Škuletić DM, Marjanović-Balaban ŽR, Vujadinović DP, Rajić DZ, Tomović VM. Gliadin proteins in muffins with quinoa flour. Food Processing: Techniques and Technology. 2024;54(1):82–92. https://doi.org/10.21603/2074-9414-2024-1-2490
- Angeli V, Silva PM, Massuela DC, Khan MW, Hamar A, Khajehei F, et al. Quinoa (Chenopodium quinoa Willd.): An overview of the potentials of the “golden grain” and socio-economic and environmental aspects of its cultivation and marketization. Foods. 2020;9(2):216. https://doi.org/10.3390/foods9020216
- Yang X, Zhu K, Guo H, Geng Y, Lv W, Wang S, et al. Characterization of volatile compounds in differently coloured Chenopodium quinoa seeds before and after cooking by headspace-gas chromatography-ion mobility spectrometry. Food Chemistry. 2021;348:1290086. https://doi.org/10.1016/j.foodchem.2021.129086
- Li X, Chen S, Yao Y, Wu N, Xu M, Zhao Y, et al. The quality characteristics formation and control of salted eggs: A review. Foods. 2022;11(19):2949. https://doi.org/10.3390/foods11192949
- Komeroski MR, de Oliveira VR. Influence of the amount and type of whey protein on the chemical, technological, and sensory quality of pasta and bakery products. Foods. 2023;12(14):2801. https://doi.org/10.3390/foods12142801
- Godefroidt T, Ooms N, Pareyt B, Brijs K, Delcour JA. Ingredient functionality during foam-type cake making: A review. Comprehensive Reviews in Food Science and Food Safety. 2019;18:1550–1562. https://doi.org/10.1111/1541-4337.12488
- Yazici GN, Ozer MS. A review of egg replacement in cake production: Effects on batter and cake properties. Trends in Food Science and Technology. 2021;111:346–359. https://doi.org/10.1016/j.tifs.2021.02.071
- Erfanian A, Rasti B. Effects of soy milk on physical, rheological, microbiological and sensory properties of cake. International Food Research Journal. 2019;26(1):237–245.
- Boukid F, Gagaoua M. Vegan egg: A future-proof food ingredient? Foods. 2022;11(2):161. https://doi.org/10.3390/foods11020161
- Nastaj M, Mleko S, Terpiłowski K, Tomczy´nska-Mleko M. Effect of sucrose on physicochemical properties of high-protein meringues obtained from whey protein isolate. Applied Sciences. 2021;11(11):4764. https://doi.org/10.3390/app11114764
- Guerreo-Ochoa MR, Pedreschi R, Chirinos R. Optimised methodology for the extraction of protein from quinoa (Chenopodium quinoa Willd.). International Journal of Food Science and Technology. 2015;50(8):1815. https://doi.org/10.1111/ijfs.12834
- Robertson JA, de Monredon FD, Dysseler P, Guillon F, Amado R, Thibault J-F. Hydration properties of dietary fibre and resistant starch: A European collaborative study. LWT – Food Science and Technology. 2000;33(2):72–79. https://doi.org/10.1006/fstl.1999.0595
- Wani AI, Sogi D, Gill SB. Physicochemical and functional properties of flours from three Black gram (Phaseolus mungo L.) cultivars. International Journal of Food Science and Technology. 2013;48(4):771. https://doi.org/10.1111/ijfs.12025
- Fallah-Delavar M, Farmani J. Recovery and characterization of enzymatic protein hydrolysates and fat from chicken skin. Journal of the American Oil Chemists' Society. 2018;95:1151–1161. https://doi.org/10.1002/aocs.12131
- Shahidi F, Han X-O, Synowiecki J. Production and characteristics of protein hydrolysates from capelin (Mallotus villosus). Food Chemistry. 1995;53(3):285–293. https://doi.org/10.1016/0308-8146(95)93934-J
- Shao Y, Kao J. Foaming properties of soy protein isolates and concentrates. International Conference on Food Security and Nutrition. 2014.
- Özhamamci I, Çakiroglu K, Ertugay MF. The effects of emulsifiers and their different forms on the physical, chemical and textural properties of sponge cakes. International Journal of Engineering Trends and Technology. 2019;67(1). https://doi.org/10.14445/22315381/IJETT-V67I1P201
- Approved Method of the American Association of Cereal Chemists. American Association of Cereal Chemists; 2000. 1200 p.
- Officials Methods of Analysis, 17th Edn. Association of Official Analytical Chemists; 2000.
- Polachini TC, Morales SAV, Filho LRP, Ribeiro EF, Saraiva LS, Basso RC. Physical properties and molecular interactions applied to food processing and formulation. Processes. 2023;11(7):2181. https://doi.org/10.3390/pr11072181
- Zhang Y, Hou R, Zhu B, Yin G, Zhang J, Zhao W, et al. Changes on the conformational and functional properties of soybean protein isolate induced by quercetin. Frontiers in Nutrition. 2022;9:966750. https://doi.org/10.3389/fnut.2022.966750
- Mizutani Y, Shibata M, Yamada S, Nambu Y, Hirotsuka M, Matsumura Y. Effects of heat treatment under low moisture conditions on the protein and oil in soybean seeds. Food Chemistry. 2019;275:577–584. https://doi.org/10.1016/j.foodchem.2018.09.139
- Hedayati S, Tehrani MM. Effect of total replacement of egg by soymilk and lecithin on physical properties of batter and cake. Food Science and Nutrition. 2018;6:1154. https://doi.org/10.1002/fsn3.656
- Li X, Wang Y-M, Sun C-F, Lv J-H, Yang Y-J. Comparative study on foaming properties of egg white with yolk fractions and their hydrolysates. Foods. 2021;10(9):2238. https://doi.org/10.3390/foods10092238
- Dakhili S, Abdolalizadeh L, Hosseini SM, Shojaee-Aliabadi S, Mirmoghtadaie L. Quinoa protein: Composition, structure and functional properties. Food Chemistry. 2019;299:125161. https://doi.org/10.1016/j.foodchem.2019.125161
- Mu J, Qi Y, Gong K, Chen Z, Brennan MA, Qianyun M, et al. Effects of quinoa flour (Chenopodium quinoa Willd) substitution on wheat flour characteristics. Current Research in Food Science. 2023;7:100556. https://doi.org/10.1016/j.crfs.2023.100556
- Tian Y, Rao H, Zhang K, Tao S, Xue W-T. Effects of different thermal processing methods on the structure and allergenicity of peanut allergen Ara h 1. Food Science and Nutrition. 2018;6:1706. https://doi.org/10.1002/fsn3.742
- Harisha R, Singh SK, Ahlawat AK, Narwal S, Jaiswal JP, Sin JB, et al. Elucidating the effects on polyphenol oxidase activity and allelic variation of polyphenol oxidase genes on dough and whole wheat-derived product color parameters. International Journal of Food Properties. 2023;26(2):2716–2731. https://doi.org/10.1080/10942912.2023.2252196
- Rashwan AK, Osma AI, Abdelshafy AM, Mo J, Chen W. Plant-based proteins: Advanced extraction technologies, interactions, physicochemical and functional properties, food and related applications, and health benefits. Critical Reviews in Food Science and Nutrition. 2023. https://doi.org/10.1080/10408398.2023.2279696
- Khalid II, Elharadallou SB. Functional properties of cowpea (Vigna ungiculata L. Walp), and lupin (Lupinus termis) flour and protein isolates. Journal of Nutrition and Food Sciences. 2023;3:234–240. https://doi.org/10.4172/2155-9600.1000234
- Salehi F. Improvement of gluten-free bread and cake properties using natural hydrocolloids: A review. Food Science and Nutrition. 2019;7:3391–3402. https://doi.org/10.1002/fsn3.1245
- Gallego C, Belorio M, Guerra-Oliveira M, Gomez M. Effects of adding chickpea and chestnut flours to layer cakes. International Journal of Food Science and Technology. 2022;57:4840–4846. https://doi.org/10.1111/ijfs.15719
- Blanco Canalis MS, Valentinuzzi MC, Acosta RH, León AE, Ribotta PD. Effects of fat and sugar on dough and biscuit behaviours and their relationship to proton mobility characterized by TD-NMR. Food and Bioprocess Technology. 2018;11:953–965. https://doi.org/10.1007/s11947-018-2063-z
- Bieniek B, Buksa K. Properties and functionality of cereal non-starch polysaccharides in breadmaking. Applied Sciences. 2023;13(4):2282. https://doi.org/10.3390/app13042282
- Vidal L, Ewigmann H, Schuster C, Alpers T, Scherf K, Jekle M, et al. Microscopic analysis of gluten network development under shear load-combining confocal laser scanning microscopy with rheometry. Journal of Texture Studies. 2023;54(4). https://doi.org/10.1111/jtxs.12796
- Romano A, Masi P, Bracciale A, Aiello A, Nicolai MA, Ferranti P. Effect of added enzymes and quinoa flour on dough characteristics and sensory quality of a gluten-free bakery product. European Food Research and Technology. 2018;244:1595–1604. https://doi.org/10.1007/s00217-018-3072-x
How to quote?
Mohamed RK, Ahmed ZS, Abozed SS. Multi-objective development of novel egg free cakes using quinoa protein and its quality attributes. 2025;13(2):276–286.
https://doi.org/10.21603/2308-4057-2025-2-638