ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Influence of blackberry juice addition on mead fermentation and quality

Introduction. Mead, one of the oldest alcoholic beverages that man consumed is obtained by fermentation of honey solution, and contains from 8 to 18% vol. ethanol. Honey can be considered as an excellent source of carbohydrates for the fermentation process, but low concentrations of other substances in the honey can slow down the process. Blackberry (Rubus fruticosus L.) contains dietary fibers, vitamin C (ascorbic acid), vitamin A, vitamin E, potassium and calcium, along with the phenolic metabolites that are a source of possible health benefits.
Study objects and methods. In this study was investigated the influence of blackberry juice addition on mead fermentation process, chemical composition and antioxidative activity. Dynamics of the fermentation process were controlled based on weighing the flasks in time on a scale every 24 h throughout the alcoholic fermentation. At the end of fermentations, oenological parameters of mead: dry matter content, pH, volatile acidity and ethanol content and reducing sugars. For the determination of antioxidative capacity the content of total phenolics, total flavonoids and total flavonols were measured and two tests were performed: DPPH and ABTS.
Results and discussion. Addition of blackberry juice had a positive effect on fermentation dynamics (almost 25% higher rate of fermentation than in control samples), and improved all physicochemical characteristics and composition of resultant meads. Also, meads with the addition of blackberry juice had a significantly higher concentration of total phenolics, total flavonoids and total flavonols and significantly stronger antioxidative properties compared to the control meads without juice addition. The highest total phenolics, total flavonoids and total flavonols content was determined in the mead with the maximum addition of blackberry juice (B20W): it reached 490.88, 50.34 and 62.57 μgQE.mL–1, respectively, and was 6-fold higher for total phenolics and total flavonoids content, and 10-fold higher for total flavonols content than in the mead without juice addition (CW). The strongest antioxidative activity was determined in the B10W mead; it accounted for 6.98 μgTE.mL–1 (DPPH assay) and 0.65 μgTE.mL–1 (ABTS assay), what was 1.5-fold and 3-fold higher, respectively, than in the mead without juice addition (CW). Conclusion. The conducted study demonstrated that the use of blackberry juice influenced the course of fermentation of meads as well as their physicochemical and antioxidative properties (positive effect on fermentation dynamics – almost 25% higher rate of fermentation than in control samples, and improvement of all physicochemical characteristics and composition of resultant meads).
Fruit, blackberry, beverages, fermentation rate, mead, antioxidant activity, kinetic model
This study is a result of the research conducted within the Project (19/6-020/961-68/18) financially supported by the Ministry for Scientific and Technological Development, Higher Education and Information Society of the Republic of Srpska, Bosnia and Herzegovina.
  1. Pereira AP, Mendes-Ferreira A, Estevinho LM, Mendes-Faia A. Improvement of mead fermentation by honey-must supplementation. Journal of the Institute of Brewing. 2015;121(3):405–410.
  2. Kempka AP, Frühauf M, Pagliarini MA, Matiello JA, Fachinello F, Prestes RC. Influence of the addition of pollen and brewer’s yeast on growth of Saccharomyces cerevisiae in honey-must. International Food Research Journal. 2015;22(3):1288–1292.
  3. Adamenko K, Kawa-Rygielska J, Kucharska AZ, Piórecki N. Characteristics of biologically active compounds in cornelian cherry meads. Molecules. 2018;23(8).
  4. Amorim TS, Lopes SDB, Bispo JAC, Bonafe CFS, de Carvalho GBM, Martínez EA. Influence of acerola pulp concentration on mead production by Saccharomyces cerevisiae AWRI 796. LWT – Food Science and Technology. 2018;97:561–569.
  5. Balogu T, Towobola O. Production and quality analysis of wine from honey and coconut milk blend using Saccharomyces cerevisiae. Fermentation. 2017;3(2).
  6. United States Department of Agriculture. FoodData Central [Internet]. [cited 2020 Apr 10]. Available from:
  7. Socha R, Pajak P, Fortuna T, Buksa K. Phenolic profile and antioxidant activity of polish meads. International Journal of Food Properties. 2015;18(12):2713–2725.
  8. Akalin H, Bayram M, Anli RE. Determination of some individual phenolic compounds and antioxidant capacity of mead produced from different types of honey. Journal of the Institute of Brewing. 2016;123(1):167–174.
  9. Czabaj S, Kawa-Rygielska J, Kucharska AZ, Kliks J. Effects of mead wort heat treatment on the mead fermentation process and antioxidant activity. Molecules. 2017;22(5).
  10. Cuenca M, Ciesa F, Romano A, Robatscher P, Scampicchio M, Biasioli F. Mead fermentation monitoring by proton transfer reaction mass spectrometry and medium infrared probe. European Food Research and Technology. 2016;242(10):1755–1762.
  11. Papuga S, Savić A, Kisin Z. Matematičko modelovanje proizvodnje etanola u toku fermentacije medovine [Mathematical modeling of ethanol production in the mead fermentation]. Glasnik Hemičara, Tehnologa i Ekologa Republike Srpske [Journal of Chemists, Technologists and Ecologists of the Republic of Srpska]. 2018;10(14)15–22. (In Serbian).
  12. Ordinance on honey and other bee products. Official Gazette of Bosnia and Herzegovina. 2009;(37).
  13. Ordinance on methods for control of honey and other bee products. Official Gazette of Bosnia and Herzegovina. 2009;(37).
  14. Compendium of international methods of wine and must analysis. Paris: Organisation Internationale de la Vigne et du Vin; 2015.
  15. Miller GL. Use of dinitrosalicylic acid reagent for determination of reducing sugar. Analytical Chemistry. 1959;31(3):426–428.
  16. Wolfe K, Wu X, Liu RH. Antioxidant activity of apple peels. Journal of Agricultural and Food Chemistry. 2003;51(3):609–614.
  17. Kumaran A, Karunakaran RJ. In vitro antioxidant activities of methanol extracts of five Phyllanthus species from India. LWT – Food Science and Technology. 2007;40(2):344–352.
  18. Ordenez AAL, Gomez JD, Vattuone MA, Isla MI. Antioxidant activities of Sechium edule (Jacq.) Swartz extracts. Food Chemistry. 2006;97(3):452–458.
  19. Liyana-Pathiranan CM, Shahidi F. Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric ph conditions. Journal of Agricultural and Food Chemistry. 2005;53(7):2433–2440.
  20. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9–10):1231–1237.
  21. Malherbe S, Bauer FF, Du Toit M. Understanding problem fermentations – A review. South African Journal of Enology and Viticulture. 2007;28(2):169–185.
  22. Pereira AP, Mendes-Ferreira A, Oliveira JM, Estevinho LM, Mendes-Faia A. High-cell-density fermentation of Saccharomyces cerevisiae for the optimisation of mead production. Food Microbiology. 2013;33(1):114–123.
  23. Kawa-Rygielska J, Adamenko K, Kucharska AZ, Szatkowska K. Fruit and herbal meads – Chemical composition and antioxidant properties. Food Chemistry. 2019;283:19–27.
  24. Gomes T, Barradas C, Dias T, Verdial J, Morais JS, Ramalhosa E, et al. Optimization of mead production using Response Surface Methodology. Food and Chemical Toxicology. 2013;59:680–686.
  25. Bonvehi JS, Coll FV. Evaluation of bitterness and astringency of polyphenolic compounds in cocoa powder. Food Chemistry. 1997;60(3):365−370.
How to quote?
Savić A, Velemir A, Papuga S, Stojković M. Influence of blackberry juice addition on mead fermentation and quality. Foods and Raw Materials. 2021;9(1):146–152.
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