ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Antagonistic effects of raffia sap with probiotics against pathogenic microorganisms

Abstract
Introduction. Probiotics are known for their beneficial properties. Numerous studies have been conducted to find advantages that probiotics can provide. This study aimed to evaluate the functional properties of raffia sap, a Cameroonian drink, fermented with probiotics by investigating its antagonistic activity against pathogenic bacteria.
Study objects and methods. The study objective was raffia sap fermented by Lactobacillus fermentum and Bifidobacterium bifidum. Box-Behnken design with four factors (seeding rates of L. fermentum and B. bifidum, temperature, and incubation time) was used to generate mathematical models. The disc diffusion method was used to evaluate an antagonistic effect of the probiotics against four pathogenic bacteria (Escherichia coli, Listeria monocytogenes, Salmonella sp., and Bacillus cereus). An optimization of mathematical models of the inhibition diameters allowed to determine the optimal conditions of antagonistic effect.
Results and discussion. The experimental data showed that zones of inhibition were 0‒21 mm for Salmonella sp., 0‒23 mm for E. coli, 0‒20 mm for L. monocytogenes, and 0‒22 mm for B. cereus. ANOVA results and the mathematical models obtained showed that L. fermentum was effective against B. cereus and B. bifidum against Salmonella sp., E. coli, and B. cereus. The optimization of the models revealed maximum zones of inhibition at the seeding rates of L. fermentum and B. bifidum of 2 and 10%, respectively, incubation time of 48 h, and temperature of 37°C.
Conclusion. Raffia sap fermented by L. fermentum and B. bifidum demonstrated antagonistic effect against pathogenic bacteria such as E. coli, L. monocytogenes, Salmonella sp., and B. cereus.
Keywords
Probiotics, antagonistic activity, pathogenic bacteria, response surface methodology, mathematical model
REFERENCES
  1. Salminen S. Human studies on probiotics: aspects of scientific documentation. Scandinavian Journal of Nutrition/ Naringsforskning. 2001;45(1):8–12.
  2. Chen H, Tian M, Chen L, Cui X, Meng J, Shu G. Optimization of composite cryoprotectant for freeze-drying Bifidobacterium bifidum BB01 by response surface methodology. Artificial Cells Nanomedicine and Biotechnology. 2019;47(1):1559–1569. https://doi.org/10.1080/21691401.2019.1603157.
  3. Schrezenmeir J, de Vrese M. Probiotics, prebiotics, and synbiotics – approaching a definition. American Journal of Clinical Nutrition. 2001;73(2):361S–364S.
  4. Heller KJ. Probiotic bacteria in fermented foods: product characteristics and starter organisms. American Journal of Clinical Nutrition. 2001;73(2):374S–379S.
  5. Tadesse G, Ephraim E, Ashenafi M. Assessment of the antimicrobial activity of lactic acid bacteria isolated from Borde and Shamita, traditional Ethiopian fermented beverages, on some foodborne pathogens and effect of growth medium on inhibitory activity. Internet Journal of Food Safety. 2005;5:13–20.
  6. Saarela M, Mogensen G, Fonden R, Matto J, Mattila-Sandholm T. Probiotic bacteria: safety, functional and technological properties. Journal of Biotechnology. 2000;84(3):197–215. https://doi.org/10.1016/s0168-1656(00)00375-8.
  7. Yu HS, Lee NK, Choi AJ, Choe JS, Bae CH, Paik HD. Antagonistic and antioxidant effect of probiotic Weissella cibaria JW15. Food Science and Biotechnology. 2019;28(3):851–855. https://doi.org/10.1007/s10068-018-0519-6.
  8. Lim SM, Im DS. Inhibitory effects of antagonistic compounds produced from Lactobacillus brevis MLK27 on adhesion of Listeria monocytogenes KCTC3569 to HT-29 cells. Food Science and Biotechnology. 2012;21(3):775–784. https://doi.org/10.1007/s10068-012-0101-6.
  9. Saxelin M, Tynkkynen S, Mattila-Sandholm T, de Vos WM. Probiotic and other functional microbes: from markets to mechanisms. Current Opinion in Biotechnology. 2005;16(2):204–211. https://doi.org/10.1016/j.copbio.2005.02.003.
  10. Ratsep M, Naaber P, Koljalg S, Smidt I, Shkut E, Sepp E. Effect of Lactobacillus plantarum strains on clinical isolates of Clostridium difficile in vitro. Journal of Probiotics and Health. 2014;2(1). https://doi.org/10.4172/2329-8901.1000119.
  11. Arqués JL, Rodríguez E, Langa S, Landete JM, Medina M. Antimicrobial activity of lactic acid bacteria in dairy products and gut: Effect on pathogens. Biomed Research International. 2015;2015. https://doi.org/10.1155/2015/584183.
  12. Kasra-Kermanshahi R, Mobarak-Qamsari E. Inhibition effect of lactic acid bacteria against food born pathogen, Listeria monocytogenes. Applied Food Biotechnology. 2015;2(4):11–19.
  13. Mbarga MJA, Desobgo ZSC, Ngouné TL, Meisam Z, Engeribo A, Maryam B. Producing probiotic beverage based on raffia sap fermented by Lactobacillus fermentum and Bifidobacterium bifidum. Research on Crops. 2019;20(3):629–634. https://doi.org/10.31830/2348-7542.2019.092.
  14. Sukhikh AS, Zakharova YuV, Yuzhalin AE, Bykov AT, Kotova TV, Poznyakovskiy VM. Criteria for standardization of probiotic components in functional food products. Foods and Raw Materials. 2018;6(2):457–466. https://doi.org/10.21603/2308-4057-2018-2-457-466.
  15. Ojo OC, Agboola SA. Evaluation of the antagonistic activity of bacteria isolated from palm wine (Raphia vinifera) on Salmonella typhi from different sources. Journal of Advances in Microbiology. 2018;13(1):1–9.
  16. Garcıa P, Rodrıguez L, Rodrıguez A, Martınez B. Food biopreservation: Promising strategies using bacteriocins, bacteriophages and endolysins. Trends in Food Science and Technology. 2010;21(8):373–382. https://doi.org/10.1016/j.tifs.2010.04.010.
  17. Devaraj NK, Suppiah S, Veettil SK, Ching SM, Lee, KW, Menon RK, et al. The effects of probiotic supplementation on the incidence of diarrhea in cancer patients receiving radiation therapy: A systematic review with meta-analysis and trial sequential analysis of randomized controlled trials. Nutrients. 2019;11(12). https:/doi.org/10.3390/nu11122886.
  18. Luquet F-M, Corrieu.G. Bactéries lactiques et probiotiques. Lavoisier; 2008. 320 p.
  19. Cintas LM, Casaus MP, Herranz C, Nes IF, Hernandez PE. Review: Bacteriocins of lactic acid bacteria. Food Science and Technology International. 2001;7(4):281–305. https://doi.org/10.1106/r8de-p6hu-clxp-5ryt.
  20. Hwang JY, Park JH. Characteristics of enterotoxin distribution, hemolysis, lecithinase, and starch hydrolysis of Bacillus cereus isolated from infant formulas and ready-to-eat foods. Journal of Dairy Science. 2015;98(3):1652–1660. https://doi.org/10.3168/jds.2014-9042.
  21. Aslim B, Yuksekdag ZN, Sarikaya E, Beyatli Y. Determination of the bacteriocin-like substances produced by some lactic acid bacteria isolated from Turkish dairy products. LWT – Food Science and Technology. 2005;38(6):691–694. https://doi.org/10.1016/j.lwt.2004.08.001.
  22. Soleimani NA, Kermanshahi RK, Yakhchali B, Sattari TN. Antagonistic activity of probiotic lactobacilli against Staphylococcus aureus isolated from bovine mastitis. African Journal of Microbiology Research. 2010;4(20):2169–2173.
  23. Callaway TR, Edrington TS, Anderson RC, Harvey RB, Genovese KJ, Kennedy CN, et al. Probiotics, prebiotics and competitive exclusion for prophylaxis against bacterial disease. Animal Health Research Reviews. 2008;9(2):217–225. https://doi.org/10.1017/S1466252308001540.
  24. Akarca G, Tomar O, Güney İ, Erdur S, Gök V. Determination of sensitivity of some food pathogens to spice extracts. Journal of Food Science and Technology. 2019;56(12):5253–5261. https://doi.org/10.1007/s13197-019-03994-1.
  25. O’Sullivan L, Ross RP, Hill C. Potential of bacteriocin-producing lactic acid bacteria for improvements in food safety and quality. Biochimie. 2002;84(5–6):593–604. https://doi.org/10.1016/s0300-9084(02)01457-8.
  26. Ju J, Xie Y, Guo Y, Cheng Y, Qian H, Yao W. The inhibitory effect of plant essential oils on foodborne pathogenic bacteria in food. Critical Reviews in Food Science and Nutrition. 2019;59(20):3281–3292. https://doi.org/10.1080/10408398.2018.1488159.
  27. You YS, Cha YJ, Choi SS. Effect of lactic acid bacteria on intestinal E. coli in Caenorhabditis elegans. Food Science and Biotechnology. 2015;24(5):1853–1858. https://doi.org/10.1007/s10068-015-0242-5.
  28. Abdelhamid AG, El-Masry SS, El-Dougdoug NK. Probiotic Lactobacillus and Bifidobacterium strains possess safety characteristics, antiviral activities and host adherence factors revealed by genome mining. EPMA Journal. 2019;10(4):337–350. https://doi.org/10.1007/s13167-019-00184-z.
  29. Poroś-Głuchowska J, Markiewicz Z. Antimicrobial resistance of Listeria monocytogenes. Acta Microbiologica Polonica. 2003;52(2):113–129.
  30. Lee VC. The antibiotic resistance crisis. Part 1: Causes and threats. P&T. 2015;40(4):277–283.
  31. Unal Turhan E, Erginkaya Z, Uney MH, Ozer EA. Inactivation effect of probiotic biofilms on growth of Listeria monocytogenes. Kafkas Universitesi Veteriner Fakultesi Dergisi. 2017;23(4):541–546. https://doi.org/10.9775/kvfd.2016.17253.
How to quote?
Mbarga MJA, Desobgo SCZ, Tatsadjieu LN, Kavhiza N, Kalisa L. Antagonistic effects of raffia sap with probiotics against pathogenic microorganisms. Foods and Raw Materials. 2021;9(1):24–31. https://doi.org/10.21603/2308-4057-2021-1-24-31
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