Abstract

Breads with proven hepatoprotective properties can make a significant contribution to preventing liver disease. This work aimed to study hepatoprotective and antioxidant effects of breads enriched with water and ethanol extracts of polyphenol-containing viburnum (Viburnum sargentii Koehne L.), magnolia-vine (Schisandra chinensis L.), and grapes (Vitis amurénsis L.). It was based on an experimental model of toxic hepatitis in mice intoxicated with carbon tetrachloride. Experimental groups of animals were fed on bread with extracts for 7 days and control groups had a bread-free diet. We analysed their body weight, liver lipid metabolism, “lipid peroxidation – antioxidant protection” system, and antiradical activity. The level of reduced glutathione and malonic dialdehyde was determined by micro-thin-layer chromatography. Superoxide dismutase, glutathione reductase, and glutathione peroxidase activity was measured to analyse the antioxidant system. The total content of common polyphenols in breads was determined by the colorimetric method with the Folin-Chocalteu reagent. The animals on a bread-free diet showed an impaired lipid metabolism and higher activity of liver enzymes. They had a 22% increase in liver weight and a 1.9 times depletion of antiradical protection (6.65 ± 0.15 Trolox units/mg protein vs. 13.15 ± 0.21 Trolox units/mg protein in the control; P < 0.001). We also registered a 2.5 times decrease in superoxide dismutase, a key enzyme of the antioxidant defence system. The animals fed on breads with the above extracts showed a statistically significant normalization of the parameters, compared to the bread-free group. We found that those breads had hepatoprotective and antioxidant effects on the animals, stabilizing their general condition and normalizing their biochemical parameters and antioxidant system.

Keywords

Bread, plant extracts, viburnum, magnolia-vine, grapes, polyphenols, antioxidant, hepatoprotector

REFERENCES

1. Postanovlenie Pravitelʹstva RF ot 15 iyulya 2010 g. N 530 “Ob utverzhdenii Pravil ustanovleniya predelʹno dopustimykh roznichnykh tsen na otdelʹnye vidy sotsialʹno znachimykh prodovolʹstvennykh tovarov pervoy neobkhodimosti, perechnya otdelʹnykh vidov sotsialʹno znachimykh prodovolʹstvennykh tovarov pervoy neobkhodimosti, v otnoshenii kotorykh mogut ustanavlivatʹsya predelʹno dopustimye roznichnye tseny, i perechnya otdelʹnykh vidov sotsialʹno znachimykh prodovolʹstvennykh tovarov, za priobretenie opredelennogo kolichestva kotorykh khozyaystvuyushchemu subʺektu, osushchestvlyayushchemu torgovuyu deyatelʹnostʹ, ne dopuskaetsya vyplata voznagrazhdeniya” [The RF Government Decree No. 530 of July 15, 2010 “Approving the Rules for establishing maximum allowable retail prices for certain types of basic foodstuffs, the List of certain types of basic foodstuffs for which maximum allowable retail prices can be established, and the List of certain types of basic foodstuffs for the acquisition of a certain amount of which the retailer is not entitled to remuneration”].
2. Lobanov VG, Slepokurova YI, Zharkova IM, Koleva TN, Roslyakov YF, Krasteva AP. Economic effect of innovative flour-based functional foods production. Foods and Raw Materials. 2018;6(2):474–482. DOI: http://doi. org/10.21603/2308-4057-2018-2-474-482.
3. Vengerovskiy AI, Markova IV. Doklinicheskoe izuchenie gepatozashchitnykh sredstv [A preclinical study of hepatoprotective agents]. Vedomosti farmakologicheskogo komiteta [Vedomosti Farmakologicheskogo Komiteta]. 1999;(2):9–12. (In Russ.).
4. Sanz, MJ, Ferrandiz ML, Cejudo M, Terencio MC, Gil B, Bustos G, et al. Influence of a series of natural flavonoids on free-radical generating systems and oxidative stress. Xenobiotica. 1994;24(7):689–699. DOI: https://doi. org/10.3109/00498259409043270.
5. Es-Safi N-E, Ghidouche S, Ducrot PH. Flavonoids: hemisynthesis, reactivity, characterization and free radical scavenging activity. Molecules. 2007;12(9):2228–2258. DOI: https://doi.org/10.3390/12092228.
6. Terao J. Dietary flavonoids as antioxidants. Forum of Nutrition. 2009;61:87–94. DOI: https://doi.org/10.1159/000212741.
7. De Souza RF, De Giovani WF. Antioxidant properties of complexes of flavonoids with metal ions. Redox Report. 2004;9(2):97–104. DOI: https://doi.org/10.1179/135100004225003897.
8. Pamplona R. Advanced lipoxidation end-products. Chemico-Biological Interactions. 2011;192(1–2):14–20. DOI: https://doi.org/10.1016/j.cbi.2011.01.007.
9. Momot TV. Prirodnye rastitelʹnye fenolʹnye soedineniya v profilaktike stressovykh narusheniy metabolicheskikh reaktsiy organizma zhivotnykh i cheloveka [Natural plant phenolic compounds in preventing stress disorders of metabolic reactions in animals and humans: doctoral thesis in medical science]. Dr. med. sci. diss. Vladivostok: Pacific State Medical University; 2018. 347 p.
10. Olszewska MA, Michel P. Antioxidant activity of inflorescences, leaves and fruits of three Sorbus species in relation to their polyphenolic composition. Natural Product Research. 2009;23(16):1507–1521. DOI: https://doi.org/10.1080/14786410802636177.
11. Kharchenko YuA, Avdonina OO, Eremenko SV. Hepatoprotective properties of new drags. Modern problems of science and education. 2012;(1):215. (In Russ.).
12. Zain MZM, Baba AS, Shori AB. Effect of polyphenols enriched from green coffee bean on antioxidant activity and sensory evaluation of bread. Journal of King Saud University – Science. 2018;30(2):278–282. DOI: https://doi.org/10.1016/j.jksus.2017.12.003.
13. Sivam AS, Sun-Waterhouse D, Perera CO, Waterhouse GIN. Exploring the interactions between blackcurrant polyphenols, pectin and wheat biopolymers in model breads; a FTIR and HPLC investigation. Food Chemistry. 2012;131(3):802–810. DOI: https://doi.org/10.1016/j.foodchem.2011.09.047.
14. Sun-Waterhouse D, Sivam AS, Cooney J, Zhou J, Perera CO, Waterhouse GIN. Effects of added fruit polyphenols and pectin on the properties of finished breads revealed by HPLC/LC-MS and Size-Exclusion HPLC. Food Research International. 2011;44(9):3047–3056. DOI: https://doi.org/10.1016/j.foodres.2011.07.022.
15. Prokopov T, Chonova V, Slavov A, Dessev T, Dimitrov N, Petkova N. Effects on the quality and health-enhancing properties of industrial onion waste powder on bread. Journal of Food Science and Technology. 2018;55(12):5091– 5097. DOI: https://doi.org/10.1007/s13197-018-3448-8.
16. Hidalgo A, Brandolini A, Čanadanović-Brunet J, Ćetković G, Tumbas Šaponjac V. Microencapsulates and extracts from red beetroot pomace modify antioxidant capacity, heat damage and colour of pseudocereals-enriched einkorn water biscuits. Food Chemistry. 2018;268:40–48. DOI: https://doi.org/10.1016/j.foodchem.2018.06.062.
17. Rico D, Martin-Diana AB, Lasa A, Aguirre, Milton-Laskibar I, de Luis DA, et al. Effect of wakame and carob pod snacks on non-alcoholic fatty liver disease. Nutrients. 2019;11(1). DOI: https://doi.org/10.3390/nu11010086.
18. Tang G-Y, Zhao C-N, Liu Q, Feng X-L, Xu X-Y, Cao S-Y, et al. Potential of grape wastes as a natural source of bioactive compounds. Molecules. 2018;23(10). DOI: https://doi.org/10.3390/molecules23102598.
19. Rico D, Alonso de Linaje A, Herrero A, Asensio-Vegas C, Miranda J, Martínez-Villaluenga C, et al. Carob by-products and seaweeds for the development of functional bread. Journal of Food Processing and Preservation. 2018;42(8). DOI: https://doi.org/10.1111/jfpp.13700.
20. Smertina ES, Fedyanina LN, Kalenik TK, Vigerina NS, Karaseva SV, Kushnerova NF, et al. Kompozitsiya dlya prigotovleniya testa dlya khlebobulochnykh izdeliy (varianty) [Bread dough composition (variants)]. Russian patent RU 2483548C2. 2013.
21. Petrov AN, Maslennikova GA. Physical and chemical aspects of vacuum drying of berry raw materials. Foods and Raw Materials. 2016;4(1):129–134. DOI: http://doi.org/10.21179/2308-4057-2016-1-129-134.
22. Parfenova TV. Obosnovanie primeneniya polifenolʹnykh kompleksov iz dikorosov kak antioksidantov dlya proizvodstva marmelada funktsionalʹnogo naznacheniya i ego tovarovednaya kharakteristika [Substantiating the use of polyphenolic complexes from wild plants as antioxidants in producing functional fruit jelly and its product characteristics]. Cand. eng. sci. diss. Khabarovsk: Pacific National University; 2006. 26 p.
23. Fomenko SE, Kushnerova NF, Sprigin VG, Parfenova TV, Kushnerova TV. Application of plant polyphenols in the composition of functional foods. The bulletin of the Far Eastern Federal University. Economics and Management. 2009;49(1):62–69. (In Russ.).
24. Parfenova TV, Kushnerova NF, Korastyleva LA, Bystrova AM. Jelly fruit candy with biological active additives from wild plants. Storage and processing of farm products. 2007;(3):70–72. (In Russ.).
25. Vershinina AG, Kushnerova NF, Lentsova LV, Parfenova TV, Kalenik TK. Vozmozhnostʹ ispolʹzovaniya novykh rastitelʹnykh dobavok iz dikorosov ussuriyskoy taygi kak antioksidantov dlya ehmulʹsionnoy pishchevoy produktsii dlitelʹnogo khraneniya [The possibility of using new plant supplements from the Ussuri taiga as antioxidants for long shelf life emulsion food products]. News of institutes of higher education. Food Technology. 2004;278(1):62–64. (In Russ.).
26. Fedyanina LN, Kalenik TK, Smertina ES, Zvyagintseva TN, Imbs TI, Shevchenko NM, et al. To the question about security of ingredients using for the making bakery products for the purpose of medioprophylactic. The bulletin of the Far Eastern Federal University. Economics and Management. 2011;60(4):82–86. (In Russ.).
27. Smertina ES, Fedyanina LN, Lyakh VA. Seaweed BAA production waste usage as the functional ingredients in baking products contents. The bulletin of the Far Eastern Federal University. Economics and Management. 2012;64(4):94– 102. (In Russ.).
28. Fedyanina LN, Smertina ES, Vigerina NS, Stepanov SV. On the efficiency of bakery products c bioactive food supplements adaptogennoe effect. Technology and merchandising of the innovative foodstuff. 2012;14(3):51–56. (In Russ.).
29. Sprygin VG, Kushnerova NF, Fomenko SE. Waste from processing the far-east wild-growing plants – perspective sources of nutritional antioxidants. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2010;12(1–3):812–815. (In Russ.).
30. Momot TV, Kushnerova NF. Justification of the choice of raw sources from far east flora for receiving the pharmaceutical preparations. Izvestia of Samara Scientific Center of the Russian Academy of Sciences. 2016;18(2):146–149. (In Russ.).
31. Sbornik tekhnologicheskikh instruktsiy dlya proizvodstva khlebobulochnykh izdeliy [Collected technological instructions for bread production]. Moscow: Preyskurantizdat; 1989. 1080 p. (In Russ.).
32. Singleton VL, Orthofer R, Lamuela-Raventós RM. Analysis of total phenols and other oxidation substrates and antioxidants by means of Folin-ciocalteu reagent. Methods in Enzymology. 1998;299:152–178. DOI: https://doi. org/10.1016/S0076-6879(99)99017-1.
33. Vegerovskiy AI, Markova IV, Saratikov AS. Metodicheskie ukazaniya po izucheniyu gepatozashchitnoy aktivnosti farmakologicheskikh veshchestv [Guidelines for studying hepatoprotective activity of pharmacological substances]. In: Khabriev RYu, editor. Rukovodstvo po ehksperimentalʹnomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv [Guidelines for an experimental (preclinical) study of new pharmacological substances]. Moscow: Meditsina; 1990. pp. 228–231. (In Russ.).
34. Khabriev RYu. Rukovodstvo po ehksperimentalʹnomu (doklinicheskomu) izucheniyu novykh farmakologicheskikh veshchestv [Guidelines for an experimental (preclinical) study of new pharmacological substances]. Moscow: Meditsina; 2005. 832 p. (In Russ.).
35. Paoletti F, Aldinucci D, Mocali A, Caparrini A. A sensitive spectrophotometric method for the determination of superoxide dismutase activity in tissue extracts. Analytical Biochemistry. 1986;154(2):536–541. DOI: https://doi. org/10.1016/0003-2697(86)90026-6.
36. Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS+ radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9–10):1231–1237. DOI: https://doi.org/10.1016/S0891-5849(98)00315-3.
37. Novgorodtseva TP, Ehndakova EhA, Yanʹkova VI. Rukovodstvo po metodam issledovaniya parametrov sistemy “perekisnoe okislenie lipidov-antioksidantnaya zashchita” v biologicheskikh zhidkostyakh [Guidelines for methods of studying the “lipid peroxidation – antioxidant protection” system parameters in biological fluids]. Vladivostok: Far Eastern Federal University; 2003. 80 p. (In Russ.).
38. Folch J, Lees M, Sloane Stanley GH. A simple method for the isolation and purification of total lipids from animal tissue. The Journal of biological chemistry. 1957;226(1):497–509.
39. Amenta JS. A rapid chemical method for quantification of lipids separated by thin-layer chromatography. Journal of Lipid Research. 1964;5(2):270–272.
40. Brattin WJ, Glende AJ, Recknagel RO. Pathological mechanisms in carbon tetrachloride hepatotoxicity. Journal of Free Radicals in Biology and Medicine. 1985;1(1):27–38. DOI: https://doi.org/10.1016/0748-5514(85)90026-1.
41. Tsuchiya H. Effects of green tea catechins on membrane fluidity. Pharmacology. 1999;59(1):34–44. DOI: https://doi.org/10.1159/000028303.
42. Maffei Facino R, Carini M, Aldini G, Calloni MT, Bombardelli E, Morazzoni P. Sparing effect of procyanidins from Vitis vinifera on vitamin E: In vitro studies. Planta Medica. 1998;64(4):343–347. DOI: https://doi. org/10.1055/s-2006-957448.
43. Young IS, Woodside JV. Antioxidants in health and disease. Journal of Clinical Pathology. 2001;54(3):176–186. DOI: https://doi.org/10.1136/jcp.54.3.176.
44. Sprygin VG, Kushnerova NF, Rakhmanin YuA. An antioxidant action produced by oligomeric anthocyanidins, isolated from viburnum, in lesions of the liver by carbon tetrachloride and prevention of its toxic effect. Hygiene and sanitation. 2003;(3):57–60. (In Russ.).
45. Kushnerova NF, Sprygin VG. Vliyanie kompleksnogo polifenolʹnogo preparata “kalifen” na protsessy vosstanovleniya biokhimicheskikh pokazateley pecheni posle porazheniya ehtilovym spirtom [The effect of the Caliphene polyphenolic complex on the recovery of liver biochemical parameters after intoxication with ethyl alcohol]. Problems of Biological, Medical and Pharmaceutical Chemistry. 2002;(4):22–26. (In Russ.).