ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Antioxidant and antimicrobial properties of oregano extract (Origani vulgaris herba L.)

Abstract
Introduction. Some ingredients of plant origin possess both antioxidant and bacteriostatic properties. If used in the food industry, they can inhibit microbiological and oxidative damage, thus increasing the shelf life of meat products. Oregano extract is one of such substances, which means that it can be used as an antioxidant and preservative. Therefore, the study of this plant has a significant theoretical and practical potential for the food industry.
Study objects and methods. The present research featured ground trimmed beef. The samples with 20% of fat tissue were used to determine the microbiological parameters, while the samples with 30% of fat were used to obtain data on oxidative stability. The control sample contained no additional ingredients. The sample with food additives was pre-treated with acidity regulators and antioxidants, namely sodium acetate E262, ascorbic acid E300, sodium ascorbate E301, sodium citrate E331, and rosemary extract E392. The sample with oregano extract was pre-treated with oregano extract (Origani vulgaris herba L.) in the ratio of 5 g of extract per 1 kg of meat. The extract had been dissolved in 100 g of water. The samples were stored at 4 ± 2°С for 12 days. A CM5 spectrophotometer (Konica Minolta, Japan) was used to determine the color characteristics. The induction period of oxidative stability was determined using an Oxitest oxidative stability analyzer (Velp Scientifica, Italy). The studies were conducted in Austria, Linz.
Results and discussion. Oregano extract stabilized the redness rating. For the sample with oregano extract, the induction period of oxidative stability was twice as long as for the control sample and the sample with antioxidants. In addition, oregano inhibited the growth of aerobic and anaerobic microorganisms.
Conclusion. The antioxidant and antimicrobial properties of oregano extract prolong the shelf life of ground beef, which makes it possible to reduce the amount of food additives.
Keywords
Meat, Origani vulgaris herba, antioxidants, colour stability, oxidative stability, antimicrobial properties
REFERENCES
  1. Domínguez R, Pateiro M, Gagaoua M, Barba FJ, Zhang W, Lorenzo JM. A comprehensive review on lipid oxidation in meat and meat products. Antioxidants. 2019;8(10). DOI: https://doi.org/10.3390/antiox8100429.
  2. Niki E, Yoshida Y, Saito Y, Noguchi N. Lipid peroxidation: mechanisms, inhibition, and biological effects. Biochemical and Biophysical Research Communications. 2005;338(1):668–676. DOI: https://doi.org/10.1016/j.bbrc.2005.08.072.
  3. Amaral AB, da Silva MV, Lannes SCS. Lipid oxidation in meat: mechanisms and protective factors – a review. Food Science and Technology. 2018;38:1–15. DOI: https://doi.org/10.1590/fst.32518.
  4. Tunieva EK, Gorbunova NA. Possibilities to use natural antioxidants. Meat Industry. 2015;(9):40–44. (In Russ.).
  5. Rather SA, Masoodi FA, Akhter R, Rather JA, Shiekh KA. Advances in use of natural antioxidants as food additives for improving the oxidative stability of meat products. Madridge Journal of Food Technology. 2016;1(1):10–17.
  6. Pennington JAT, Fisher RA. Classification of fruits and vegetables. Journal of Food Composition and Analysis. 2009;22(1):S23–S31. DOI: https://doi.org/10.1016/j.jfca.2008.11.012.
  7. Karre L, Lopez K, Getty KJK. Natural antioxidants in meat and poultry products. Meat Science. 2013;94(2):220–227. DOI: https://doi.org/10.1016/j.meatsci.2013.01.007.
  8. El-Zainy AR, Morsy AE, Sedki AG, Mosa NM. Polyphenols grape seeds extract as antioxidant and antimicrobial in beef sausage. International Journal of Current Science. 2016;19(2):112–121.
  9. Zhang H, Wu J, Guo X. Effects of antimicrobial and antioxidant activities of spice extracts on raw chicken meat quality. Food Science and Human Wellness. 2016;5(1):39–48. DOI: https://doi.org/10.1016/j.fshw.2015.11.003.
  10. Banerjee R, Verma AK, Das AK, Rajkumar V, Shewalkar AA, Narkhede HP. Antioxidant effects of broccoli powder extract in goat meat nuggets. Meat Science. 2012;91(2):179–184. DOI: https://doi.org/10.1016/j.meatsci.2012.01.016.
  11. Ham Y-K, Hwang KE, Song D-H, Kim YJ, Shin DJ, Kim KI, et al. Lotus (Nelumbo nucifera) rhizome as an antioxidant dietary fiber in cooked sausage: effects on physicochemical and sensory characteristics. Korean Journal for Food Science of Animal Resources. 2017;37(2):219–227. DOI: https://doi.org/10.5851/kosfa.2017.37.2.219.
  12. García-Lomillo J, Gonzalez-San Jose ML, Del Pino-García R, Ortega-Heras M, Muñiz-Rodríguez P. Antioxidant effect of seasonings derived from wine pomace on lipid oxidation in refrigerated and frozen beef patties. LWT – Food Science and Technology. 2017;77:85–91. DOI: https://doi.org/10.1016/j.lwt.2016.11.038.
  13. Munekata PES, Paseto Fernandes RDP, de Melo MP, Trindade MA, Lorenzo JM. Influence of peanut skin extract on shelf-life of sheep patties. Asian Pacific Journal of Tropical Biomedicine. 2016;6(7):586–596. DOI: https://doi.org/10.1016/j.apjtb.2016.05.002.
  14. Andrés AI, Petrón MJ, Adámez JD, López M, Timón ML. Food by-products as potential antioxidant and antimicrobial additives in chill stored raw lamb patties. Meat Science. 2017;129:62–70. DOI: https://doi.org/10.1016/j.meatsci.2017.02.013.
  15. Aminzare M, Hashemi M, Ansarian E, Bimkar M, Azar HH, Mehrasbi MR, et al. Using natural antioxidants in meat and meat products as preservatives: A review. Advances in Animal and Veterinary Sciences. 2019;7(5):417–426. DOI: https://doi.org/10.17582/journal.aavs/2019/7.5.417.426.
  16. Lisitsyn AB, Tuniyeva EK, Gorbunova NA. Oxidation of lipids: the mechanism, dynamics, inhibition. All about the meat. 2015;(1):10–15. (In Russ.).
  17. Johns AM, Birkinshaw LH, Ledward DA. Catalysts of lipid oxidation in meat products. Meat Science. 1989;25(3):209–220. DOI: https://doi.org/10.1016/0309-1740(89)90073-9.
  18. Rhee KS, Ziprin YA. Lipid oxidation in retail beef, pork and chicken muscles as affected by concentrations of heme pigments and non-heme iron and microsomal enzymic lipid peroxidation activity. Journal of Food Biochemistry. 1987;11(1):1–15. DOI: https://doi.org/10.1111/j.1745-4514.1987.tb00109.x.
  19. Baron CP, Andersen HJ. Myoglobin-induced lipid oxidation. A review. Journal of Agricultural and Food Chemistry. 2002;50(14):3887–3897. DOI: https://doi.org/10.1021/jf011394w.
  20. Tichivangana JZ, Morrissey PA. Metmyoglobin and inorganic metals as pro-oxidants in raw and cooked muscle systems. Meat Science. 1985;15(2):107–116. DOI: https://doi.org/10.1016/0309-1740(85)90051-8.
  21. Borella TG, Peccin MM, Mazon JM, Roman SS, Cansian RL, Soares MBA. Effect of rosemary (Rosmarinus officinalis) antioxidant in industrial processing of frozen-mixed hamburger during shelf life. Journal of Food Processing and Preservation. 2019;43(9). DOI: https://doi.org/10.1111/jfpp.14092.
  22. Mozaffari Nejad AS, Shabani S, Bayat M, Hosseini SE. Antibacterial effect of garlic aqueous extract on Staphylococcus aureus in hamburger. Jundishapur Journal of Microbiology. 2014;7(11):1–5. DOI: https://doi.org/10.5812/jjm.13134.
  23. Baker IA, Alkass JE, Saleh HH. Reduction of oxidative rancidity and microbial activities of the karadi lamb patties in freezing storage using natural antioxidant extracts of rosemary and ginger. International Journal of Agricultural and Food Research. 2013;2(1):31–42.
  24. Koné AP, Desjardins Y, Gosselin A, Cinq-Mars D, Guay F, Saucier L. Plant extracts and essential oil product as feed additives to control rabbit meat microbial quality. Meat Science. 2019;150:111–121. DOI: https://doi.org/10.1016/j.meatsci.2018.12.013.
  25. Trindade RA, Mancini-Filho J, Villavicencio ALCH. Natural antioxidants protecting irradiated beef burgers from lipid oxidation. LWT – Food Science and Technology. 2010;43(1):98–104. DOI: https://doi.org/10.1016/j.lwt.2009.06.013
  26. Tsigarida E, Skandamis P, Nychas G-JE. Behaviour of Listeria monocytogenes and autochthonous flora on meat stored under aerobic, vacuum and modified atmosphere packaging conditions with or without the presence of oregano essential oil at 5°C. Journal of Applied Microbiology. 2000;89(6):901–909. DOI: https://doi.org/10.1046/j.1365-2672.2000.01170.x.
  27. Skandamis PN, Nychas G-JE. Effect of oregano essential oil on microbiological and physico-chemical attributes of minced meat stored in air and modified atmospheres. Journal of Applied Microbiology. 2001;91(6):1011–1022. DOI: https://doi.org/10.1046/j.1365-2672.2001.01467.x.
  28. Moreno Y, Arteaga-Minano HL. Natural conservation of guinea pig (Cavia porcellus) meat vacuum packed: Oregano essential oil effect on the physicochemical, microbiological and sensory characteristics. Scientia Agropecuaria. 2018;9(4):467–476. DOI: https://doi.org/10.17268/sci.agropecu.2018.04.01.
  29. Radha krishnan K, Babuskin S, Azhagu Saravana Babu P, Sasikala M, Sabina K, Archana G, et al. Antimicrobial and antioxidant effects of spice extracts on the shelf life extension of raw chicken meat. International Journal of Food Microbiology. 2014;171:32–40. DOI: https://doi.org/10.1016/j.ijfoodmicro.2013.11.011.
  30. Rojas MC, Brewer MS. Effect of natural antioxidants on oxidative stability of frozen, vacuum-packaged beef and pork. Journal of Food Quality. 2008;31(2):173−188. DOI: https://doi.org/10.1111/j.1745-4557.2008.00196.x.
  31. Cattelan MG, de Castilhos MBM, Sales PJP, Hoffmann FL. Antibacterial activity of oregano essential oil against foodborne pathogens. Nutrition and Food Science. 2013;43(2):169–174. DOI: https://doi.org/10.1108/00346651311313544.
  32. Lambert RJW, Skandamis PN, Coote PJ, Nychas G-JE. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. Journal of Applied Microbiology. 2001;91(3):453–462. DOI: https://doi.org/10.1046/j.1365-2672.2001.01428.x.
  33. Govaris A, Solomakos N, Pexara A, Chatzopoulou PS. The antimicrobial effect of oregano essential oil, nisin and their combination against Salmonella Enteritidis in minced sheep meat during refrigerated storage. International Journal of Food Microbiology. 2010;137(2–3):175–180. DOI: https://doi.org/10.1016/j.ijfoodmicro.2009.12.017.
  34. Cui H, Zhang C, Li C, Lin L. Antibacterial mechanism of oregano essential oil. Industrial Crops and Products. 2019;139. DOI: https://doi.org/10.1016/j.indcrop.2019.111498.
  35. Agrimonti C, White JC, Tonetti S, Marmiroli N. Antimicrobial activity of cellulosic pads amended with emulsions of essential oils of oregano, thyme and cinnamon against microorganisms in minced beef meat. International Journal of Food Microbiology. 2019;305. DOI: https://doi.org/10.1016/j.ijfoodmicro.2019.108246.
  36. Burt S. Essential oils: their antibacterial properties and potential applications in foods – a review. International Journal of Food Microbiology. 2004;94(3):223–253. DOI: https://doi.org/10.1016/j.ijfoodmicro.2004.03.022.
  37. Karabagias I, Badeka A, Kontominas MG. Shelf life extension of lamb meat using thyme or oregano essential oils and modified atmosphere packaging. Meat Science. 2011;88(1):109–116. DOI: https://doi.org/10.1016/j.meatsci.2010.12.010.
How to quote?
Oleynikov VV. Antioxidant and antimicrobial properties of oregano extract (Origani vulgaris herba L.). Foods and Raw Materials. 2020;8(1):84–90. DOI: http://doi.org/10.21603/2308-4057-2020-1-84-90
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