ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Genoprotective, antimutagenic, and antioxidant effect of methanolic leaf extract of Rhamnus alaternus L. from the Bissa mountains in Algeria

Abstract
Rhamnus alaternus L. is a Rhamnaceae shrub and a popular traditional medicine in Algeria. The present research objective was to investigate the antioxidant, genotoxic, and antigenotoxic properties of R. alaternus methanolic leaf extract. Antiradical scavenging activity was tested by α, α-diphenyl-β-picrylhydrazyl free radical scavenging and β-carotene bleaching method. DNA damage and repair were measured by the Allium cepa test with sodium azide as a mutagenic agent. Mitotic index and chromosomal aberrations were calculated by microscopy of meristem roots stained with 2% carmine acetic. The methanolic extract of R. alaternus leaves inhibited the free radical DPPH (IC50 = 0.74 ± 0.3 mg/mL) and prevented the oxidation of β-carotene (50.71 ± 4.17%). The root phenotyping showed that sodium azide changed their color and shape, decreased their stiffness, and significantly reduced their length. The roots treated with both R. alaternus leaf extract and sodium azide demonstrated a better root growth. The roots treated with the methanolic extract were much longer than the control roots (P < 0.001). The microscopy images of root meristem treated with the sodium azide mitodepressant agent showed significant chromosomal aberrations, which indicated a disruption of the cell cycle. The R. alaternus leaf extract appeared to have a beneficial effect on cytotoxicity. The antioxidant properties of R. alaternus L. makes this plant an excellent genoportector.
Keywords
Rhamnus alaternus L. , antioxidant activity , Allium cepa , chromosomal aberrations , antigenotoxicity , mitotic index
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How to quote?
Gadouche Leila, Zidane Azdinia, Zerrouki Khayra, Ababou Adda, Bachir Elazaar Ibtissem, Merabet Dounya, Henniche Wissam, Ikhlef Samah Genoprotective, antimutagenic, and antioxidant effect of methanolic leaf extract of Rhamnus alaternus L. from the Bissa mountains in Algeria. Foods and Raw Materials, 2022, vol. 10, no. 2, pp. 196-205
DOI
http://doi.org/10.21603/2308-4057-2022-2-530
Publisher
Kemerovo State University
https://kemsu.ru
ISSN
2308-4057 (Print) /
2310-9599 (Online)
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