Abstract

The paper deals with urgent issues of development of resource-saving methods of biotechnological processes intensification. The main purpose of the work is to show the efficiency of using extremely low doses of physical factors impact in food biotechnology. Acoustic and electric treatment was in various modes. Impact capacity did not exceed 10-4 W/kg. The duration of exposure ranged from 5 to 15 min. Barley grains enzymes and lactic acid microorganisms were subjected to treatment. Impact of vibration as a physical factor, its frequency is considered as a priority in controlling growth and biochemical processes in biological objects. Impact frequency in the range of 50–10000 Hz influences the activity of hydrolytic enzymes in bimodal way. It is presented in the article. The coincidence of the frequency ranges of the maximum activity of enzymes in the model reactions with the ones in the grain of barley under the treatment of alternating current and sound is noted. Improvement of all indexes of germinating barley and improvement of the quality of the finished malt were observed in these ranges. Low-intensity acoustic treatment at a frequency of 2000 Hz contributed to an increase in β-galactosidase activity of the CT-95 Str. thermophilus strain. Selected ST-95 Str. thermophilus strain was used in the starter composition for the experimental sample production of fermented melted milk. The fermentation process intensification was observed. The lactose content of this product is 30% less in comparison with the feedstock. The use of traditional starter without a selected strain can reduce the lactose content by an average of 7.5%. The results of biotechnological processes modification by means of the treatment of enzymes and extremely low doses of physical factors impact used in meat technologies are observed. Thus, the prospects of using low-intensity physical effects of vibration in the development of innovative food technologies are substantiated. It is also promising to use these technologies in making absolutely new food products with different qualities.

Keywords

Food biotechnology, biotransformation, hydrolytic enzymes, barley grain, lactic acid microorganisms, the impact of physical factors

REFERENCES

1. Tompkins P. and Berd K. Taynaya zhiznʹ rasteniy [The secret life of plants]. Moscow: Homeopathic Medicine Publ., 2006. 444 p. (In Russ.).
2. Rogov I.A. Ehlektrofizicheskie metody obrabotki pishchevykh produktov [Electrophysical methods of processing food products]. Moscow: Agropromizdat Publ., 1988. 582 p. (In Russ.).
3. Betskii O.V., Devyatkov N.D., and Kislov V.V. Low Intensity Millimeter Waves in Medicine and Biology. Critical Reviews in Biomedical Engineering, 2000, vol. 28, no. 1–2, pp. 247–268.
4. Rogov I.A. Biologicheskie aspekty mikrovolnovykh pishchevykh tekhnologiy [Biological aspects of microwave food technologies]. Electronic processing of materials, 2000, no. 5, pp. 115–125. (In Russ.).
5. Rubin A.B. Fundamentals of Biophysics. Beverly: Scrivener Publ., 2014. 226 p. DOI: https://doi.org/10.1002/9781118842768.
6. Slavetskaya M.B. and Kapay N.A. Sverkhmalye dozy biologicheski aktivnykh veshchestv kak osnova lekarstvennykh preparatov [Ultra-low doses of biologically active substances as the basis of drugs]. Moscow: Advertising group Department–72 Publ., 2011. 168 p. (In Russ.).
7. Voeykov V.L. and Korotkov K.G. Novaya nauka o vode [New science of water]. Moscow: DrK Publ., 2017. 212 p. (In Russ.).
8. Epstein O.I. Ultralow doses (History of the research). Moscow: RAMS Publ., 2009. 302 p. (In Russ.).
9. Burlakova E.B., Konradov A.A., and Maltseva E.L. Deystvie sverkhmalykh doz biologicheski aktivnykh veshchestv i nizkointensivnykh fizicheskikh faktorov [The impact of ultra-low dose of biologically active substances and low-intensity physical factors]. Russian Journal of Physical Chemistry B: Focus on Physics, 2003, vol. 22, no. 2, p. 21–40. (In Russ.).
10. Kulichenko E.N. Chudo sverkhmalykh doz [Miracle of ultra-low doses]. Tyumen: Russian week Publ., 2014. 152 p. (In Russ.).
11. Konovalov A.I. and Ryzhkina I.S. Highly diluted aqueous solutions: Formation of nano-sized molecular assemblies (nanoassociates). Geochemistry International, 2014, vol. 52, no. 13, pp. 1207–1226. DOI: https://doi.org/10.1134/S0016702914130072.
12. Ivanenko G.F. and Burlakova E.B. The effect of low dose-rate radiation on thiol-disulfide system and lipid antioxidants in blood plasma of exposed persons. Radiation and Risk, 2017, vol. 26, no. 4, pp. 111–123.
13. Ratushnyak A.A., Ratushnyak A.Yu., and Trushin M.V. Development of Methodology for Detection of Ecological Maximum Permissible Concentration of Xenobiotics (On the Example of Pyrethroid Insecticides). World Applied Sciences Journal, 2013, vol. 24, no. 12, pp. 1616–1620. DOI:https://doi.org/10.5829/idosi.wasj.2013.24.12.13298.
14. Binhi V.N. and Savin A.V. Effects of weak magnetic fields on biological systems: Physical aspects. Physics–Uspekhi, 2003, vol. 46, no. 3, pp. 259–291. DOI: https://doi.org/10.1070/PU2003v046n03ABEH001283.
15. Zhorina L.V. and Zmievskaya G.N. Osnovy vzaimodeystviya fizicheskikh poley s bioobʺektami [Bases of interaction of physical fields with biological objects]. Moscow: N.E. Bauman MSTU Publ., 2014. 374 p.
16. Narziss L. and Back W. Die Bierbrauerei: Band 1 - Die Technologie Der Malzbereitung. Germany: Wiley-Vch, 2012. 910 p.
17. Aksenova B.K., Rebezov M.B., and Topuriya G.M. Kontrolʹ kachestva moloka i molochnykh produktov [Quality control of milk and dairy products]. Moscow: SSU Publ., 2013. 212 p.
18. Tihomirova N.A. Present day situation and prospects of functional products developments. Dairy industry, 2009, no. 7, pp. 5–8.
19. Zhang Z., Xu F., Liu Z., Wang R., and Wen T. MicroRNA-Mediated Regulation in Biological Systems with Oscillatory Behavior. BioMed Research International, 2013, vol. 2013, p. 7. DOI: https://doi.org/10.1155/2013/285063.
20. Fung E.A, Wong W.W., Suen J.K., et al. A synthetic gene-metabolic. Nature, 2005, vol. 435, no. 7038, pp. 118–122.
21. Sticker J., Cookson S., and Bennet M.R. A fast, robust and tunable synthetic gene oscillator. Nature, 2008, vol. 456, no. 7221, pp. 516–519. DOI: https://doi.org/10.1038/nature07389
22. Uriu K. Genetic oscillators in development. Development, Growth & Differentiation, 2016, vol. 58, no. 1, pp. 16–30. DOI: https://doi.org/10.1111/dgd.12262.
23. Amiranashvili A., Schnellbächer N.D., and Schwarz U.S. Stochastic switching between multistable oscillation patterns of the Min-system. New Journal of Physics, 2016, vol. 18, no. 9.DOI: https://doi.org/10.1088/1367-2630/18/9/093049.
24. Danino T., Mondrogon-Palomino O., Tsimring L., and Hasty J. A synchronized guorum of genetic cloks. Nature, 2010, vol. 463, no. 7279, pp. 326–330. DOI: https://doi.org/10.1038/nature08753.
25. Meledina T.V., Prokhorchik T.P., and Kuznetsova L.I. Biokhimicheskie protsessy pri proizvodstve soloda [Biochemical processes in the production of malt]. St. Petersburg: NIU ITMO; IhiBT Publ., 2013. 89 p.
26. Astakhova L., Babich O., Prosekov A., et al. Short chain fatty acids (SCFA) reprogram gene expression in human malignant epithelial and lymphoid cells. Plos One, 2016, vol. 11, no. 7. DOI: https://doi.org/10.1371/journal.pone.0154102.
27. Danilchuk T.N., Rogov I.A., and Abdrashitova G.G. Innovative Technologies for Processing Meat Raw Material Using Low-Intensity Acoustic Exposure. Storage and processing of farm products, 2017, no. 4, pp. 15–17.
28. Rubin A. and Riznichenko G. Matematical Biophisics. Springer Series in Chemical Physics, 2014yu p. 273
29. Romanovsky Yu.M., Kargovsky A.V., and Ebeling W. Models of active Brownian motors on internal oscillations. European Physical Journal: Special Topics, 2013, vol. 222, no. 10, pp. 2465–2479.
30. Riznichenko G.Yu. Mathematical Models in Biophisics. Encyclopedia of Life Support Systems (EOLSS), Mathematical models of life support systems, 2015, vol. 2, pp. 137–192.
31. Rogov I.A. and Danilchuk T.N. Mekhanizm biologicheskikh ehffektov krayne nizkikh doz kolebatelʹnykh i volnovykh vozdeystviy v oblasti zvukovykh chastot. Chastʹ II. Fiziko-khimicheskaya modelʹ vliyaniya nizkointensivnykh fizicheskikh faktorov na aktivnostʹ gidroliticheskikh fermentov [The Mechanism of biological effects of extremely low-dose vibrational and wave impact in the field of sound frequencies.Part II. Physical-chemical model of low-intensity physical factors impact on the activity of hydrolytic enzymes]. Elektronnaya obrabotka materialov, 2017, vol. 53, no. 1, pp. 70–77. (In Russ.).