Abstract

Introduction. Wild-crafting leads to the local extinction of many medicinal plants that are rich in phenolic substances. In vitro cultivation of cells and organs of higher plants can be the optimal solution to this problem. The research objective was to study the biosynthetic activity of in vitro extracts of wild Siberian plants.
Study objects and methods. The study featured callus, cell suspension, and hairy root extracts of such Siberian medicinal plants as Eleutherococcus senticosus, Codonopsis pilosula, Platanthera bifolia, and Saposhnikovia divaricata. They were obtained by in vitro cultivation using modified nutrient media of Murashige and Skoog and Gamborg. The content of secondary metabolites was studied using the methods of thin-layer and high-performance liquid chromatography. A set of in vitro experiments tested the antioxidant and antimicrobial activity of the extracts.
Results and discussion. All the samples demonstrated a high content of secondary metabolites of phenolic nature. Flavonoglycosides, apigenin, and rutin were found to be the predominant biologically active substances in the callus extracts. Flavonoglycosides dominated in the suspension extracts. The root extracts contained more caffeic acid, rutin, ecdysteroids, quercetin, apigenin, cardiofolin, and coleofolide than the callus and suspension cultures. The list of prevailing secondary metabolites in the root extracts included rutin, apigenin, coleofolide, and quercetin. All the extracts showed antimicrobial and antioxidant activity.
Conclusion. All the extracts demonstrated good antioxidant and antimicrobial properties. Therefore, they can be used for the production of pharmaceuticals and biologically active food supplements as they can be helpful against infectious diseases, as well as oncological, cardiovascular, and neurodegenerative diseases linked to oxidative stress.

Keywords

Callus culture, cell suspension culture, hairy roots, medicinal plant, secondary metabolite, phenolic substances, antioxidant, oxidative stress, antimicrobial properties, extraction

FUNDING

The research was financed by the Ministry of Science and Higher Education of the Russian Federation (Minobrnauka) as part of the Federal Program “Research in Priority Directions for the Development of the Russian Science and Technology Complex for 2014-2020”, agreement No. 075-02-2018-223, November 26, 2018, and agreement No. 075-15- 2019-1362, June 14, 2019 (project identification number: RFMEFI57718X0285).

REFERENCES

1. Roleira FMF, Valera CL, Costa SC, Tavares-da-Silva EJ. Phenolic derivatives from medicinal herbs and plant extracts: anticancer effects and synthetic approaches to modulate biological activity. Studies in Natural Products Chemistry. 2018;57:115–156. https://doi.org/10.1016/B978-0-444-64057-4.00004-1.
2. Khan SS, Cazzonelli CI, Kurata H, Li CG, Badsha B, Munch G, et al. Advancing herbal medicine through metabolic pathway analysis in plants and animals for routine pharmacological assessments. Advances in Integrative Medicine. 2019;6:S106–S107. https://doi.org/10.1016/j.aimed.2019.03.307.
3. Erst AA, Erst AS, Shmakov AI. In vitro propagation of rare species Rhodiola rosea from Altai Mountains. Turczaninowia. 2018;21(4):78–86. https://doi.org/10.14258/turczaninowia.21.4.9.
4. Fibrich BD, Lall N. Maximizing medicinal plants: Steps to realizing their full potential. In: Lall N, editor. Medicinal plants for holistic health and well-being. Academic Press; 2017. pp. 297–300. https://doi.org/10.1016/B978-0-12-812475-8.00010-X.
5. Volenzo T, Odiyo J, Integrating endemic medicinal plants into the global value chains: The ecological degradation challenges and opportunities. Heliyon. 2020;6(9). https://doi.org/10.1016/j.heliyon.2020.e04970.
6. Borrell JS, Dodwrth S, Forest F, Perz-Escobar OA, Lee MA, Mattana E, et al. The climatic challenge: Which plants will people use in the next century? Environmental and Experimental Botany. 2020;170. https://doi.org/10.1016/j.envexpbot.2019.103872.
7. Arya SS, Rookes JE, Cahill DM, Lenka SK. Next-generation metabolic engineering approaches towards development of plant cell suspension cultures as specialized metabolite producing biofactories. Biotechnology Advances. 2020;45. https://doi.org/10.1016/j.biotechadv.2020.107635.
8. Ramakrishna W, Kumaria A, Rahman N, Mandave P. Anticancer activities of plant secondary metabolites: Rice callus suspension culture as a new paradigm. Rice Science. 2021;28(1):13–30. https://doi.org/10.1016/j.rsci.2020.11.004.
9. Cardoso JC, de Oliveira MEBS, Cardoso FCI. Advances and challenges on the in vitro production of secondary metabolites from medicinal plants. Horticultura Brasileira. 2019;37(2):124–132. https://doi.org/10.1590/s0102-053620190201.
10. Garia-Perez P, Lozano-Milo E, Gallego PP, Tojo C, Losada-Barreiro S, Bravo-Diaz C. Plant antioxidants in food emulsions. In: Milani J, editor. Some new aspects of colloidal systems in food. IntechOpen; 2018. pp. 11–29. https://doi.org/10.5772/intechopen.79592.
11. Babich O, Prosekov A, Zaushintsena A, Sukhikh A, Dyshlyuk L, Ivanova S. Identification and quantification of phenolic compounds of Western Siberia Astragalus danicus in different regions. Heliyon. 2019;5(8). https://doi.org/10.1016/j.heliyon.2019.e02245.
12. Kuznecov KV, Gorshkov GI. Siberian ginseng (Eleutherococcus senticosus) – adaptogen, stimulants functions animals and immunomodulators. International Journal of Applied and Fundamental Research. 2016;(11–3):477–485. (In Russ.).
13. Sun Q-L, Li Y-X, Cui Y-S, Jiang S-L, Dong C-X, Du J. Structural characterization of three polysaccharides from the roots of Codonopsis pilosula and their immunomodulatory effects on RAW264.7 macrophages. International Journal of Biological Macromolecules. 2019;130:556–563. https://doi.org/10.1016/j.ijbiomac.2019.02.165.
14. Zhang P, Hu L, Bai R, Zheng X, Ma Y, Gao X, et al. Structural characterization of a pectic polysaccharide from Codonopsis pilosula and its immunomodulatory activities in vivo and in vitro. International Journal of Biological Macromolecules. 2017;104:1359–1369. https://doi.org/10.1016/j.ijbiomac.2017.06.023.
15. Abramchuk AV. Rare and disappearing kinds medicinal plants of flora of the secondary Urals. Vestnik Biotekhnologii [Bulletin of Biotechnology]. 2018;17(3). (In Russ.).
16. Bolʹshaya illyustrirovannaya ehntsiklopediya. Lekarstvennye rasteniya [Great visual encyclopedia. Medicinal plants]. St. Petersburg: Kristall, SZKEHO; 2017. 224 p. (In Russ.).
17. Chun JM, Kim HS, Lee AY, Kim S-H, Kim HK. Anti-inflammatory and antiosteoarthritis effects of Saposhnikovia divaricata ethanol extract: in vitro and in vivo studies. Evidence-based Complementary and Alternative Medicine. 2016;2016. https://doi.org/10.1155/2016/1984238.
18. Jiang XL, Jin MY, Piao XC, Yin CR, Lian ML. Fed-batch culture of Oplopanax elatus adventitious roots: Feeding medium selection through comprehensive evaluation using an analytic hierarchy process. Biochemical Engineering Journal. 2021;167. https://doi.org/10.1016/j.bej.2021.107927.
19. Chen L, Cai Y, Liu X, Guo C, Sun S, Wu C, et al. Soybean hairy roots produced in vitro by Agrobacterium rhizogenesmediated transformation. Crop Journal. 2018;6(2):162–171. https://doi.org/10.1016/j.cj.2017.08.006.
20. Lone SA, Wani SH, Tafazul M. Cost effective protocol for in vitro propagation of Adhatoda vasica Nees. Using modified tissue culture media. International Journal of Advanced Science and Research. 2017;2(2):67–69.
21. Wang D, Wang H, Gu L. The antidepressant and cognitive improvement activities of the traditional Chinese herb Cistanche. Evidence-based Complementary and Alternative Medicine. 2017;2017. https://doi.org/10.1155/2017/3925903.
22. Bonamidan BSR, Weis GCC, da Rosa JR, Assmann CE, Alves AO, Longhi P, et al. Effects of caffeic acid on oxidative balance and cancer. In: Preedy VR, Patel VB, editors. Cancer. Oxidative stress and dietary antioxidants. Academic Press; 2021. pp. 291–300. https://doi.org/10.1016/B978-0-12-819547-5.00026-2.
23. Lima VN, Oliveira-Tintino CDM, Santos ES, Morais LP, Tintino SR, Freitas TS, et al. Antimicrobial and enhancement of the antibiotic activity by phenolic compounds: Gallic acid, caffeic acid and pyrogallol. Microbial Pathogenesis. 2016;99:56–61. https://doi.org/10.1016/j.micpath.2016.08.004.
24. Santos JFS, Tintino SR, de Freitas TS, Campina FF, Menezes IRA, Siqueira-Junior JP, et al. In vitro e in silico evaluation of the inhibition of Staphylococcus aureus efflux pumps by caffeic and gallic acid. Comparative Immunology, Microbiology and Infectious Diseases. 2018;57:22–28. https://doi.org/10.1016/j.cimid.2018.03.001.
25. Davatgaran-Taghipour Y, Masoomzadeh S, Farzaei MH, Bahramsoltani R, Karimi-Soureh Z, Rahimi R, et al. Polyphenol nanoformulations for cancer therapy: experimental evidence and clinical perspective. International Journal of Nanomedicine. 2017;12:2689–2702. https://doi.org/10.2147/IJN.S131973.
26. Xiong H, Wang J, Ran Q, Lou G, Peng C, Gan Q, et al. Hesperidin: A therapeutic agent for obesity. Drug Design, Development and Therapy. 2019;13:3855–3866. https://doi.org/10.2147/DDDT.S227499.
27. Orsavová J, Hlaváčová I, Mlček J, Snopek L, Mišurcová L. Contribution of phenolic compounds, ascorbic acid and vitamin E to antioxidant activity of currant (Ribes L.) and gooseberry (Ribes uva-crispa L.) fruits. Food Chemistry. 2019;284:323–333. https://doi.org/10.1016/j.foodchem.2019.01.072.
28. Nugroho A, Choi JS, Seong SH, Song B-M, Park K-S, Park H-J. Isolation of flavonoid glycosides with cholinesterase inhibition activity and quantification from Stachys japonica. Natural Product Sciences. 2018;24(4):259–265. https://doi.org/10.20307/nps.2018.24.4.259.
29. Wang W, Sun C, Mao L, Ma P, Liu F, Yang J, et al. The biological activities, chemical stability, metabolism and delivery systems of quercetin: A review. Trends in Food Science and Technology. 2016;56:21–38. https://doi.org/10.1016/j.tifs.2016.07.004.
30. Jarial R, Shard A, Thakur S, Sakinah M, Zularisam AW, Rezania S, et al. Characterization of flavonoids from fern Cheilanthes tenuifolia and evaluation of antioxidant, antimicrobial and anticancer activities. Journal of King Saud University – Science. 2018;30(4):425–432. https://doi.org/10.1016/j.jksus.2017.04.007.
31. Agustini FD, Arozal W, Louisa M, Siswanto S, Soetikno V, Nafrialdi N, et al. Cardioprotection mechanism of mangiferin on doxorubicin-induced rats: Focus on intracellular calcium regulation. Pharmaceutical Biology. 2016;54(7):1289–1297. https://doi.org/10.3109/13880209.2015.1073750.