ISSN 2308-4057 (Print),
ISSN 2310-9599 (Online)

Mycotoxin-contaminated food and feed in Saudi Arabia: review of occurrence and toxicity

Abstract
Introduction. Mycotoxins are reported to have a considerable impact on the health of consumers. There has been plentiful research into the effects of mycotoxins, fungi, and bacteria on the safety and quality of food and feed. This research paper reviews the literature on mycotoxins.
Study objects and methods. The study focused on mycotoxins in food and feed produced in Saudi Arabia. We reviewed literature on the occurrence and health impacts of foodborne mycotoxins. We also studied the presence of mycotoxins in herbs, nuts, cereals, dried fruits and vegetables, infant formulas and baby foods, as well as dairy products. Finally, the paper offers a review of mycotoxin analysis methods.
Results and discussion. The findings showed that mycotoxins attract a lot of scientific interest in Saudi Arabia. Certain types of mycotoxins (zearalenone, aflatoxins) and fungi (Aspergillus flavus, Penicillium chrysogenum, and Aspergillus niger) were common in the samples (isolates) of products tested in the Saudi regions. Furthermore, the researchers used different techniques of analysis such as the HPLC method, dilution plate method, thin layer chromatography, total plate count method, and seed-plate method to detect, identify, and isolate mycotoxins.
Conclusion. Most importantly, the results showed that mycotoxins have serious health impacts on consumers and most of the contamination cases are caused by improper storage conditions and/or inappropriate handling and harvesting practices.
Keywords
Toxins, fungi, herbs, nuts, cereals, dried fruits, vegetables, baby foods, dairy products, health, food contamination
REFERENCES
  1. Ben Taheur F, Kouidhi B, Al Qurashi YMA, Ben Salah-Abbes J, Chaieb K. Review: Biotechnology of mycotoxins detoxification using microorganisms and enzymes. Toxicon. 2019;160:12–22. https://doi.org/10.1016/j.toxicon.2019.02.001.
  2. Zain ME. Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society. 2011;15(2):129–144. https://doi.org/10.1016/j.jscs.2010.06.006.
  3. Nasser LA. Fungal contamination of white cheese at the stage of consumption in Saudi Arabia. Pakistan Journal of Biological Science. 2001;4(6):733–735. https://doi.org/10.3923/pjbs.2001.733.735.
  4. Shawish RR, Al-Humam NA. Contamination of beef products with staphylococcal classical enterotoxins in Egypt and Saudi Arabia. GMS Hygiene and Infection Control. 2016;11. https://doi.org/10.3205/dgkh000268.
  5. Nasser LA. Molecular identification of isolated fungi, microbial and heavy metal contamination of canned meat products sold in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences. 2015;22(5):513–520. https://doi.org/10.1016/j.sjbs.2014.08.003.
  6. Bokhari FM. Spices mycobiota and mycotoxins available in Saudi Arabia and their abilities to inhibit growth of some toxigenic fungi. Mycobiology. 2007;35(2):47–53.
  7. Al-Hindi RR, Aly SE, Hathout AS, Alharbi MG, Al-Masaudi S, Al-Jaouni SK, et al. Isolation and molecular characterization of mycotoxigenic fungi in agarwood. Saudi Journal of Biological Sciences. 2018;25(8):1781–1787. https://doi.org/10.1016/j.sjbs.2017.07.008.
  8. Bokhari FM. Mycotoxins and toxigenic fungi in Arabic coffee beans in Saudi Arabia. Toxicology and Applied Pharmacology. 2004;197(3):246.
  9. Alsalah D, Al-Jassim N, Timraz K, Hong PY. Assessing the groundwater quality at a Saudi Arabian agricultural site and the occurrence of opportunistic pathogens on irrigated food produce. International Journal of Environmental Research and Public Health. 2015;12(10):12391–12411. https://doi.org/10.3390/ijerph121012391.
  10. El Sheikha AF. Food safety issues in Saudi Arabia. Nutrition and Food Technology. 2015;1(1). https://doi.org/10.16966/2470-6086.103.
  11. Alwakeel SS. Molecular identification of isolated fungi from stored apples in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences. 2013;20(4):311–317. https://doi.org/10.1016/j.sjbs.2013.05.002.
  12. Bokhari FM. Implications of fungal infections and mycotoxins in camel diseases in Saudi Arabia. Saudi Journal of Biological Sciences. 2010;17(1):73–81. https://doi.org/10.1016/j.sjbs.2009.12.011.
  13. Hessain AM, Al-Arfaj AA, Zakri AM, El-Jakee JK, Al-Zogibi OG, Hemeg HA, et al. Molecular characterization of Escherichia coli O157: H7 recovered from meat and meat products relevant to human health in Riyadh, Saudi Arabia. Saudi Journal of Biological Sciences. 2015;22(6):725–729. https://doi.org/10.1016/j.sjbs.2015.06.009.
  14. Al-Jaal B, Salama S, Al-Qasmi N, Jaganjac M. Mycotoxin contamination of food and feed in the Gulf Cooperation Council countries and its detection. Toxicon. 2019;171:43–50. https://doi.org/10.1016/j.toxicon.2019.10.003.
  15. Abudabos AM, Al-Atiyat RM, Khan RU. A survey of mycotoxin contamination and chemical composition of distiller’s dried grains with solubles (DDGS) imported from the USA into Saudi Arabia. Environmental Science and Pollution Research. 2017;24(18):15401–15405. https://doi.org/10.1007/s11356-017-9130-2.
  16. Alwakeel S, Nasser L. Microbial contamination and mycotoxins from nuts in Riyadh, Saudi Arabia. American Journal of Food Technology. 2011;6(8):613–630. https://doi.org/10.3923/ajft.2011.613.630.
  17. Aly MM, Al-Seeni MN, Qusti SY, El-Sawi NM. Mineral content and microbiological examination of some white cheese in Jeddah, Saudi Arabia during summer 2008. Food and Chemical Toxicology. 2010;48(11):3031–3034. https://doi.org/10.1016/j.fct.2010.05.016.
  18. El Tawila MM, Neamatallah A, Serdar SA. Incidence of aflatoxins in commercial nuts in the holy city of Mekkah. Food Control. 2013;29(1):121–124. https://doi.org/10.1016/j.foodcont.2012.06.004.
  19. Abdel-Gawad KM, Zohri AA. Fungal flora and mycotoxins of six kinds of nut seeds for human consumption in Saudi Arabia. Mycopathologia. 1993;124(1):55–64. https://doi.org/10.1007/BF01103057.
  20. Al Husnain L, AlKahtani M. Molecular heterogeneity in the 18s DNA gene of Alternaria sp. and Fusarium sp. producing mycotoxins in rice and maize grains. Saudi Journal of Biological Sciences. 2019;26(2):368–372. https://doi.org/10.1016/j.sjbs.2017.12.006.
  21. Al Khalifa AS, Ahmad D. Determination of key elements by ICP-OES in commercially available infant formulae and baby foods in Saudi Arabia. African Journal of Food Science. 2010;4(7):464–468.
  22. Alghuthaymi MA, Bahkali AH. Toxigenic profiles and trinucleotide repeat diversity of Fusarium species isolated from banana fruits. Biotechnology and biotechnological equipment. 2015;29(2):324–330. https://doi.org/10.1080/13102818.2014.995519.
  23. Al-Kahtarii DF. Isolation of fungi and their mycotoxin extract from stored wheat and other grains importer in Saudi Arabia. American Journal of Food Technology. 2014;9(7):370–376. https://doi.org/10.3923/ajft.2014.370.376.
  24. Farag RMMA, AlAyobi D, Kwon H-J, El-Ansary A. Relationship between aflatoxin b1 exposure and etiology of liver disease in Saudi Arabian patients. Journal of Pure and Applied Microbiology. 2018;12(3):1147–1153. https://doi.org/10.22207/JPAM.12.3.13.
  25. Eshelli M, Qader MM, Jambi EJ, Hursthouse AS, Rateb ME. Current status and future opportunities of omics tools in mycotoxin research. Toxins. 2018;10(11). https://doi.org/10.3390/toxins10110433.
  26. Almoammar H, Bahkali AH, Khiyami MA, Abd-Elsalam KA. Fungal infestation and mycotoxin contamination in camel feedstuffs. Fungal Genomics and Biology. 2014;4(1). https://doi.org/10.4172/2165-8056.1000115.
  27. Yassin MA, El-Samawaty AR, Bahkali A, Moslem M, Abd-Elsalam KA, Hyde KD. Mycotoxin-producing fungi occurring in sorghum grains from Saudi Arabia. Fungal Diversity. 2010;44(1):45–52. https://doi.org/10.1007/s13225-010-0058-9.
  28. Bokhari FM. Mycobiota associated with foodstuffs commodities spread in Jeddah, Saudi Arabia with special reference to Aspergillus flavus. Assiut Veterinary Medical Journal. 2001;45:94–108.
  29. Hashem M, Alamri S. Contamination of common spices in Saudi Arabia markets with potential mycotoxin-producing fungi. Saudi Journal of Biological Sciences. 2010;17(2):167–175. https://doi.org/10.1016/j.sjbs.2010.02.011.
  30. Bokhari FM, Aly MM. Unexpected hazard due to Fumonisins contaminating herbal teas used traditionally by Saudi people. African Journal of Microbiology Research. 2013;7(1):35–40. https://doi.org/10.5897/AJMR12.776.
  31. Al-Hazmi NA. Determination of patulin and ochratoxin A using HPLC in apple juice samples in Saudi Arabia. Saudi Journal of Biological Sciences. 2010;17(4):353–359. https://doi.org/10.1016/j.sjbs.2010.06.006.
  32. Gherbawy YA, Elhariry HM, Bahobial AAS. Mycobiota and mycotoxins (aflatoxins and ochratoxin) associated with some Saudi date palm fruits. Foodborne Pathogens and Disease. 2012;9(6):561–567. https://doi.org/10.1089/fpd.2011.1085.
  33. Ouf SA, Basher AH, Mohamed AAH. Inhibitory effect of double atmospheric pressure argon cold plasma on spores and mycotoxin production of Aspergillus niger contaminating date palm fruits. Journal of the Science of Food and Agriculture. 2015;95(15):3204–3210. https://doi.org/10.1002/jsfa.7060.
  34. AlFaris N, Wabaidur SM, Alothman ZA, Altamimi JZ, Aldayel TS. Fast and efficient immunoaffinity column cleanup and liquid chromatography–tandem mass spectrometry method for the quantitative analysis of aflatoxins in baby food and feeds. Journal of Separation Science. 2020;43(11):2079–2087. https://doi.org/10.1002/jssc.201901307.
  35. Rahmani J, Alipour S, Miri A, Fakhri Y, Riahi SM, Keramati H, et al. The prevalence of aflatoxin M1 in milk of Middle East region: a systematic review, meta-analysis and probabilistic health risk assessment. Food and Chemical Toxicology. 2018;118:653–666. https://doi.org/10.1016/j.fct.2018.06.016.
  36. Abdallah MIM, Bazalou MS, Al-Julaifi MZ. Determination of aflatoxin M1 concentrations in full-fat cow’s UHT milk sold for consumption in Najran-Saudi regarding its public health significance. Egyptian Journal of Applied Sciences. 2012;27(3):40–54.
  37. Contaminants and toxins in food and feed [Internet]. [cited 2020 Jun 22]. Available from: https://members.wto.org/crnattachments/2019/SPS/KWT/19_2112_00_e.pdf.
  38. Alhussaini MS. Mycobiota and mycotoxins of nuts and some dried fruits from Saudi Arabia. Journal of American Science. 2012;8(12):525–534.
  39. Mahmoud MA, Al-Othman MR, Abd El-Aziz ARM. Mycotoxigenic fungi contaminating corn and sorghum grains in Saudi Arabia. Pakistan Journal of Botany. 2013;45(5):1831–1839.
  40. Al-Julaifi MZ, Al-Falih AM. Detection of trichothecenes in animal feeds and foodstuffs during the years 1997 to 2000 in Saudi Arabia. Journal of Food Protection. 2001;64(10):1603–1606. https://doi.org/10.4315/0362-028X-64.10.1603.
  41. Abdel-Wahhab MA, Aly SE. Antioxidant property of Nigella sativa (black cumin) and Syzygium aromaticum (clove) in rats during aflatoxicosis. Journal of Applied Toxicology. 2005;25(3):218–223. https://doi.org/10.1002/jat.1057.
How to quote?
Althagafi AM, Alshegifi HM, Qussyier TS, Tobaiqy M, Mariod A. Mycotoxin-contaminated food and feed in Saudi Arabia: review of occurrence and toxicity. Foods and Raw Materials. 2021;9(1):174–183. https://doi. org/10.21603/2308-4057-2021-1-174-183
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